Protein-imprinted polymers: how far have “plastic antibodies” come?

Antibodies are highly selective and sensitive, making them the gold standard for recognition affinity tools. However, their production cost is high and their downstream processing is time-consuming. Molecularly imprinted polymers (MIPs) are tailor-made by incorporating specific molecular recognition...

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Detalles Bibliográficos
Autores: Resina, Maria Leonor Matos|||0000-0003-4216-8349, Alemán Llansó, Carlos|||0000-0003-4462-6075, Castelo Ferreira, Frederico, Esteves, Teresa
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/393239
Acceso en línea:https://hdl.handle.net/2117/393239
https://dx.doi.org/10.1016/j.biotechadv.2023.108220
Access Level:acceso abierto
Palabra clave:Polymers
Molecularly imprinted polymers
Nanoparticles
Artificial antibodies
Biomimetics
Biosensors
Biomolecules
Diagnostics
Selective targeting
Drug delivery
Polímers
Àrees temàtiques de la UPC::Enginyeria química
Descripción
Sumario:Antibodies are highly selective and sensitive, making them the gold standard for recognition affinity tools. However, their production cost is high and their downstream processing is time-consuming. Molecularly imprinted polymers (MIPs) are tailor-made by incorporating specific molecular recognition sites in their structure, thus translating into receptor-like activity mode of action. The interest in molecular imprinting technology, applied to biomacromolecules, has increased in the past decade. MIPs, produced using biomolecules as templates, commonly referred to as “plastic antibodies” or “artificial receptors”, have been considered as suitable cheaper and easy to produce alternatives to antibodies. Research on MIPs, designed to recognize proteins or peptides is particularly important, with potential contributions towards biomedical applications, namely biosensors and targeted drug delivery systems. This mini review will cover recent advances on (bio)molecular imprinting technology, where proteins or peptides are targeted or mimicked for sensing and therapeutic applications. Polymerization methods are reviewed elsewhere, being out of the scope of this review. Template selection and immobilization approaches, monomers and applications will be discussed, highlighting possible drawbacks and gaps in research.