Spatial organization of the nim1-wee1-cdc2 network in Schizosaccharomyces pombe.

In Schizosaccharomyces pombe the onset of mitosis is regulated by a network of protein kinases and phosphatases. The M-phase inducing Cdc2-Cdc13 cyclin-dependent kinase is inhibited by Weel tyrosine kinase and activated by Cdc25 phosphatase. Weel is negatively regulated by Niml protein kinase. Here,...

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Detalles Bibliográficos
Autores: Wu, Lin, Shiozaki, Kazuhiro, Aligué i Alemany, Rosa Maria, Russell, Paul
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:1996
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/33752
Acceso en línea:https://hdl.handle.net/2445/33752
Access Level:acceso abierto
Palabra clave:Citologia
Llevats
Cèl·lules eucariotes
Proteïnes quinases
Cytology
Yeast
Eukaryotic cells
Protein kinases
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spelling Spatial organization of the nim1-wee1-cdc2 network in Schizosaccharomyces pombe.Wu, LinShiozaki, KazuhiroAligué i Alemany, Rosa MariaRussell, PaulCitologiaLlevatsCèl·lules eucariotesProteïnes quinasesCytologyYeastEukaryotic cellsProtein kinasesIn Schizosaccharomyces pombe the onset of mitosis is regulated by a network of protein kinases and phosphatases. The M-phase inducing Cdc2-Cdc13 cyclin-dependent kinase is inhibited by Weel tyrosine kinase and activated by Cdc25 phosphatase. Weel is negatively regulated by Niml protein kinase. Here, we describe investigations aimed at better understanding the role of Niml in the mitotic control. The most important finding to emerge from these studies is that Weel and Niml have different patterns of intracellular localization. Immunofluorescence confocal microscopy has revealed that Niml is localized in the cytoplasm, whereas its substrate Weel is predominantly localized in the nucleus. Previous studies showed that the Cdc2-Cdc13 complex is located in the nucleus. Diversion of Niml to the nucleus, accomplished by addition of the SV40 nuclear localization signal, caused the advancement of M, confirming that Niml has restricted access to Weel in vivo. We propose that the intracellular distribution of Niml and Weel may serve to coordinate the regulation of nuclear Cdc2-Cdc13 with cytoplasmic growth.American Society for Cell Biology1996info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/33752Articles publicats en revistes (Biomedicina)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC276023/pdf/mbc00018-0095.pdfMolecular Biology of the Cell, 1996, vol. 7, num. 11, p. 1749-1758cc-by-nc-sa (c) Wu L. et al., 1996http://creativecommons.org/licenses/by-nc-sa/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/337522026-05-27T06:46:51Z
dc.title.none.fl_str_mv Spatial organization of the nim1-wee1-cdc2 network in Schizosaccharomyces pombe.
title Spatial organization of the nim1-wee1-cdc2 network in Schizosaccharomyces pombe.
spellingShingle Spatial organization of the nim1-wee1-cdc2 network in Schizosaccharomyces pombe.
Wu, Lin
Citologia
Llevats
Cèl·lules eucariotes
Proteïnes quinases
Cytology
Yeast
Eukaryotic cells
Protein kinases
title_short Spatial organization of the nim1-wee1-cdc2 network in Schizosaccharomyces pombe.
title_full Spatial organization of the nim1-wee1-cdc2 network in Schizosaccharomyces pombe.
title_fullStr Spatial organization of the nim1-wee1-cdc2 network in Schizosaccharomyces pombe.
title_full_unstemmed Spatial organization of the nim1-wee1-cdc2 network in Schizosaccharomyces pombe.
title_sort Spatial organization of the nim1-wee1-cdc2 network in Schizosaccharomyces pombe.
dc.creator.none.fl_str_mv Wu, Lin
Shiozaki, Kazuhiro
Aligué i Alemany, Rosa Maria
Russell, Paul
author Wu, Lin
author_facet Wu, Lin
Shiozaki, Kazuhiro
Aligué i Alemany, Rosa Maria
Russell, Paul
author_role author
author2 Shiozaki, Kazuhiro
Aligué i Alemany, Rosa Maria
Russell, Paul
author2_role author
author
author
dc.subject.none.fl_str_mv Citologia
Llevats
Cèl·lules eucariotes
Proteïnes quinases
Cytology
Yeast
Eukaryotic cells
Protein kinases
topic Citologia
Llevats
Cèl·lules eucariotes
Proteïnes quinases
Cytology
Yeast
Eukaryotic cells
Protein kinases
description In Schizosaccharomyces pombe the onset of mitosis is regulated by a network of protein kinases and phosphatases. The M-phase inducing Cdc2-Cdc13 cyclin-dependent kinase is inhibited by Weel tyrosine kinase and activated by Cdc25 phosphatase. Weel is negatively regulated by Niml protein kinase. Here, we describe investigations aimed at better understanding the role of Niml in the mitotic control. The most important finding to emerge from these studies is that Weel and Niml have different patterns of intracellular localization. Immunofluorescence confocal microscopy has revealed that Niml is localized in the cytoplasm, whereas its substrate Weel is predominantly localized in the nucleus. Previous studies showed that the Cdc2-Cdc13 complex is located in the nucleus. Diversion of Niml to the nucleus, accomplished by addition of the SV40 nuclear localization signal, caused the advancement of M, confirming that Niml has restricted access to Weel in vivo. We propose that the intracellular distribution of Niml and Weel may serve to coordinate the regulation of nuclear Cdc2-Cdc13 with cytoplasmic growth.
publishDate 1996
dc.date.none.fl_str_mv 1996
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/33752
url https://hdl.handle.net/2445/33752
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC276023/pdf/mbc00018-0095.pdf
Molecular Biology of the Cell, 1996, vol. 7, num. 11, p. 1749-1758
dc.rights.none.fl_str_mv cc-by-nc-sa (c) Wu L. et al., 1996
http://creativecommons.org/licenses/by-nc-sa/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-sa (c) Wu L. et al., 1996
http://creativecommons.org/licenses/by-nc-sa/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Cell Biology
publisher.none.fl_str_mv American Society for Cell Biology
dc.source.none.fl_str_mv Articles publicats en revistes (Biomedicina)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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