COP1 destabilizes DELLA proteins in Arabidopsis

DELLA transcriptional regulators are central components in the control of plant growth responses to the environment. This control is considered to be mediated by changes in the metabolism of the hormones gibberellins (GAs), which promote the degradation of DELLAs. However, here we show that warm tem...

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Detalles Bibliográficos
Autores: Blanco-Touriñán, Noel, Legris, Martina, Minguet, Eugenio G., Costigliolo-Rojas, Cecilia, Nohales, María-Ángeles, Iniesto Sánchez, Elisa, García-León, Marta, Pacín, Manuel, Heucken, Nicole, Blomeier, Tim, Locascio, Antonella, Cerný, Martin, Esteve-Bruna, David, Díez-Díaz, Mónica, Brzobohatý, Břetislav, Frerigmann, Henning, Zurbriggen, Matías D., Kay, Steve A., Rubio, Vicente, Blázquez, Miguel Ángel, Casal, Jorge J., Alabadí, David
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/228844
Acceso en línea:http://hdl.handle.net/10261/228844
Access Level:acceso abierto
Palabra clave:Shade avoidance
Thermomorphogenesis
Environment
Gibberellin
Growth
Descripción
Sumario:DELLA transcriptional regulators are central components in the control of plant growth responses to the environment. This control is considered to be mediated by changes in the metabolism of the hormones gibberellins (GAs), which promote the degradation of DELLAs. However, here we show that warm temperature or shade reduced the stability of a GA-insensitive DELLA allele in Arabidopsis thaliana. Furthermore, the degradation of DELLA induced by the warmth preceded changes in GA levels and depended on the E3 ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1). COP1 enhanced the degradation of normal and GA-insensitive DELLA alleles when coexpressed in Nicotiana benthamiana. DELLA proteins physically interacted with COP1 in yeast, mammalian, and plant cells. This interaction was enhanced by the COP1 complex partner SUPRESSOR OF phyA-105 1 (SPA1). The level of ubiquitination of DELLA was enhanced by COP1 and COP1 ubiquitinated DELLA proteins in vitro. We propose that DELLAs are destabilized not only by the canonical GA-dependent pathway but also by COP1 and that this control is relevant for growth responses to shade and warm temperature.

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