Resequencing theVrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spike

Six-rowed spike 1 (Vrs1) is a gene of major importance for barley breeding and germplasm management as it is the main gene determining spike row-type (2-rowed vs. 6-rowed). This is a widely used DUS trait, and has been often associated to phenotypic traits beyond spike type. Comprehensive re-sequenc...

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Autores: Casas, Ana M., Contreras-Moreira, Bruno, Cantalapiedra, Carlos P., Sakuma, Shun, Gracia, María Pilar, Moralejo, Marian, Molina-Cano, José Luis, Komatsuda, Takao, Igartua, Ernesto
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
Repositorio:IRTA Pubpro. Open Digital Archive
OAI Identifier:oai:repositori.irta.cat:20.500.12327/412
Acceso en línea:http://hdl.handle.net/20.500.12327/412
https://doi.org/10.1007/s11032-018-0816-z
Access Level:acceso abierto
Palabra clave:633
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spelling Resequencing theVrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spikeCasas, Ana M.Contreras-Moreira, BrunoCantalapiedra, Carlos P.Sakuma, ShunGracia, María PilarMoralejo, MarianMolina-Cano, José LuisKomatsuda, TakaoIgartua, Ernesto633Six-rowed spike 1 (Vrs1) is a gene of major importance for barley breeding and germplasm management as it is the main gene determining spike row-type (2-rowed vs. 6-rowed). This is a widely used DUS trait, and has been often associated to phenotypic traits beyond spike type. Comprehensive re-sequencing Vrs1 revealed three two-rowed alleles (Vrs1.b2; Vrs1.b3; Vrs1.t1) and four six-rowed (vrs1.a1; vrs1.a2; vrs1.a3; vrs1.a4) in the natural population. However, the current knowledge about Vrs1 alleles and its distribution among Spanish barley subpopulations is still underexploited. We analyzed the gene in a panel of 215 genotypes, made of Spanish landraces and European cultivars. Among 143 six-rowed accessions, 57 had the vrs1.a1 allele, 83 were vrs1.a2, and three showed the vrs1.a3 allele. Vrs1.b3 was found in most two-rowed accessions, and a new allele was observed in 7 out of 50 two-rowed Spanish landraces. This allele, named Vrs1.b5, contains a ‘T’ insertion in exon 2, originally proposed as the causal mutation giving rise to the six-row vrs1.a2 allele, but has an additional upstream deletion that results in the change of 15 amino acids and a potentially functional protein. We conclude that eight Vrs1 alleles (Vrs1.b2, Vrs1.b3, Vrs1.b5, Vrs1.t1, vrs1.a1, vrs1.a2, vrs1.a3, vrs1.a4) discriminate two and six-rowed barleys. The markers described will be useful for DUS identification, plant breeders, and other crop scientists.info:eu-repo/semantics/acceptedVersionSpringerProducció VegetalCultius Extensius Sostenibles201920192018info:eu-repo/semantics/article25application/pdfhttp://hdl.handle.net/20.500.12327/412https://doi.org/10.1007/s11032-018-0816-zreponame:IRTA Pubpro. Open Digital Archiveinstname:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)InglésMolecular BreedingMICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2010-21929/ES/DESCUBRIMIENTO DE NUEVA VARIABILIDAD PARA LA MEJORA DE LA CEBADA EN ESPAÑA/MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2013-48756-R/ES/DESCUBRIMIENTO Y UTILIZACION DE LA VARIABILIDAD GENETICA QUE DETERMINA LA ADAPTACION DE LA CEBADA MEDIANTE HERRAMIENTAS GENETICAS, GENOMICAS/INIA/Programa Nacional de Proyectos de Investigación Fundamental/RFP2012-00015-00-00/ES/Mantenimiento de las colecciones de germoplasma en semilla de la Estación Experimental de Aula Dei (CSIC)/INIA/Programa Nacional de Proyectos de Investigación Fundamental/RTA2012-00033-C03-02/ES/Obtención de variedades de cebada de calidad con amplia adaptación en España/MICINN/Programa Nacional de Internacionalización de la I+D/EUI2009-04075/ES/EXPLOITING GENETIC VARIATION FOR RESISTANCE TO IMPORTANT PATHOGENS IN BARLEY/MICINN/Programa Nacional de Formación de Recursos Humanos/BES-2011-045905/ES/ /Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:repositori.irta.cat:20.500.12327/4122026-06-16T08:51:17Z
dc.title.none.fl_str_mv Resequencing theVrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spike
title Resequencing theVrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spike
spellingShingle Resequencing theVrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spike
Casas, Ana M.
633
title_short Resequencing theVrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spike
title_full Resequencing theVrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spike
title_fullStr Resequencing theVrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spike
title_full_unstemmed Resequencing theVrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spike
title_sort Resequencing theVrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spike
dc.creator.none.fl_str_mv Casas, Ana M.
Contreras-Moreira, Bruno
Cantalapiedra, Carlos P.
Sakuma, Shun
Gracia, María Pilar
Moralejo, Marian
Molina-Cano, José Luis
Komatsuda, Takao
Igartua, Ernesto
author Casas, Ana M.
author_facet Casas, Ana M.
Contreras-Moreira, Bruno
Cantalapiedra, Carlos P.
Sakuma, Shun
Gracia, María Pilar
Moralejo, Marian
Molina-Cano, José Luis
Komatsuda, Takao
Igartua, Ernesto
author_role author
author2 Contreras-Moreira, Bruno
Cantalapiedra, Carlos P.
Sakuma, Shun
Gracia, María Pilar
Moralejo, Marian
Molina-Cano, José Luis
Komatsuda, Takao
Igartua, Ernesto
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Producció Vegetal
Cultius Extensius Sostenibles
dc.subject.none.fl_str_mv 633
topic 633
description Six-rowed spike 1 (Vrs1) is a gene of major importance for barley breeding and germplasm management as it is the main gene determining spike row-type (2-rowed vs. 6-rowed). This is a widely used DUS trait, and has been often associated to phenotypic traits beyond spike type. Comprehensive re-sequencing Vrs1 revealed three two-rowed alleles (Vrs1.b2; Vrs1.b3; Vrs1.t1) and four six-rowed (vrs1.a1; vrs1.a2; vrs1.a3; vrs1.a4) in the natural population. However, the current knowledge about Vrs1 alleles and its distribution among Spanish barley subpopulations is still underexploited. We analyzed the gene in a panel of 215 genotypes, made of Spanish landraces and European cultivars. Among 143 six-rowed accessions, 57 had the vrs1.a1 allele, 83 were vrs1.a2, and three showed the vrs1.a3 allele. Vrs1.b3 was found in most two-rowed accessions, and a new allele was observed in 7 out of 50 two-rowed Spanish landraces. This allele, named Vrs1.b5, contains a ‘T’ insertion in exon 2, originally proposed as the causal mutation giving rise to the six-row vrs1.a2 allele, but has an additional upstream deletion that results in the change of 15 amino acids and a potentially functional protein. We conclude that eight Vrs1 alleles (Vrs1.b2, Vrs1.b3, Vrs1.b5, Vrs1.t1, vrs1.a1, vrs1.a2, vrs1.a3, vrs1.a4) discriminate two and six-rowed barleys. The markers described will be useful for DUS identification, plant breeders, and other crop scientists.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/412
https://doi.org/10.1007/s11032-018-0816-z
url http://hdl.handle.net/20.500.12327/412
https://doi.org/10.1007/s11032-018-0816-z
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Molecular Breeding
MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2010-21929/ES/DESCUBRIMIENTO DE NUEVA VARIABILIDAD PARA LA MEJORA DE LA CEBADA EN ESPAÑA/
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2013-48756-R/ES/DESCUBRIMIENTO Y UTILIZACION DE LA VARIABILIDAD GENETICA QUE DETERMINA LA ADAPTACION DE LA CEBADA MEDIANTE HERRAMIENTAS GENETICAS, GENOMICAS/
INIA/Programa Nacional de Proyectos de Investigación Fundamental/RFP2012-00015-00-00/ES/Mantenimiento de las colecciones de germoplasma en semilla de la Estación Experimental de Aula Dei (CSIC)/
INIA/Programa Nacional de Proyectos de Investigación Fundamental/RTA2012-00033-C03-02/ES/Obtención de variedades de cebada de calidad con amplia adaptación en España/
MICINN/Programa Nacional de Internacionalización de la I+D/EUI2009-04075/ES/EXPLOITING GENETIC VARIATION FOR RESISTANCE TO IMPORTANT PATHOGENS IN BARLEY/
MICINN/Programa Nacional de Formación de Recursos Humanos/BES-2011-045905/ES/ /
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 25
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:IRTA Pubpro. Open Digital Archive
instname:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
instname_str Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
reponame_str IRTA Pubpro. Open Digital Archive
collection IRTA Pubpro. Open Digital Archive
repository.name.fl_str_mv
repository.mail.fl_str_mv
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