Filtered potential unreal huge proteins [Dataset]

An often-overlooked aspect of biology is formed by the outliers of the protein length distribution, specifically those proteins with more than 5000 amino acids, which we refer to as huge proteins (HPs). By examining UniprotKB, we discovered more than 41 000 HPs throughout the tree of life, with the...

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Detalles Bibliográficos
Autores: Amaral, Anibal S., Devos, Damien P.
Tipo de recurso: conjunto de datos
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/385326
Acceso en línea:http://hdl.handle.net/10261/385326
Access Level:acceso abierto
Palabra clave:Protein length distribution
5000 amino acids
Ribosomomal peptide synthesis
div >< p
Recognition surface proteins
Key cellular processes
Proteomes
Polyketide synthesis
Planctomycetota </
Elusimicrobiota </
Ubiquitin ligases
Potential impact
Overlooked aspect
Neglected giants
Majority found
Huge proteins
Host attachment
Homologous sequences
Highest propensity
Higher tendency
Functions related
Functional groups
Findings underscore
Examining uniprotkb
Ecological roles
Cytoskeleton organization
Average eukaryote
id ES_6014d53bb21a366e7ea1ba83184a2a8a
oai_identifier_str oai:digital.csic.es:10261/385326
network_acronym_str ES
network_name_str España
repository_id_str
spelling Filtered potential unreal huge proteins [Dataset]Amaral, Anibal S.Devos, Damien P.Protein length distribution5000 amino acidsRibosomomal peptide synthesisdiv >< pRecognition surface proteinsKey cellular processesProteomesPolyketide synthesisPlanctomycetota </Elusimicrobiota </Ubiquitin ligasesPotential impactOverlooked aspectNeglected giantsMajority foundHuge proteinsHost attachmentHomologous sequencesHighest propensityHigher tendencyFunctions relatedFunctional groupsFindings underscoreExamining uniprotkbEcological rolesCytoskeleton organizationAverage eukaryoteAn often-overlooked aspect of biology is formed by the outliers of the protein length distribution, specifically those proteins with more than 5000 amino acids, which we refer to as huge proteins (HPs). By examining UniprotKB, we discovered more than 41 000 HPs throughout the tree of life, with the majority found in eukaryotes. Notably, the phyla with the highest propensity for HPs are Apicomplexa and Fornicata. Moreover, we observed that certain bacteria, such as Elusimicrobiota or Planctomycetota, have a higher tendency for encoding HPs, even more than the average eukaryote. To investigate if these macro-polypeptides represent “real” proteins, we explored several indirect metrics. Additionally, orthology analyses reveals thousands of clusters of homologous sequences of HPs, revealing functional groups related to key cellular processes such as cytoskeleton organization and functioning as chaperones or as E3-ubiquitin ligases in eukaryotes. In the case of bacteria, the major clusters have functions related to non-ribosomomal peptide synthesis/polyketide synthesis, followed by pathogen-host attachment or recognition surface proteins. Further exploration of the annotations for each HPs supported the previously identified functional groups. These findings underscore the need for further investigation of the cellular and ecological roles of these HPs and their potential impact on biology and biotechnology.Peer reviewedPublic Library of ScienceConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252024info:eu-repo/semantics/datasethttp://purl.org/coar/resource_type/c_ddb1Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/385326reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésAmaral, Anibal S.; Devos, Damien P. The neglected giants: Uncovering the prevalence and functional groups of huge proteins in proteomes. https://doi.org/10.1371/journal.pcbi.1012459 . http://hdl.handle.net/10261/379969https://doi.org/10.1371/journal.pcbi.1012459.s007Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3853262026-05-22T06:33:51Z
dc.title.none.fl_str_mv Filtered potential unreal huge proteins [Dataset]
title Filtered potential unreal huge proteins [Dataset]
spellingShingle Filtered potential unreal huge proteins [Dataset]
Amaral, Anibal S.
Protein length distribution
5000 amino acids
Ribosomomal peptide synthesis
div >< p
Recognition surface proteins
Key cellular processes
Proteomes
Polyketide synthesis
Planctomycetota </
Elusimicrobiota </
Ubiquitin ligases
Potential impact
Overlooked aspect
Neglected giants
Majority found
Huge proteins
Host attachment
Homologous sequences
Highest propensity
Higher tendency
Functions related
Functional groups
Findings underscore
Examining uniprotkb
Ecological roles
Cytoskeleton organization
Average eukaryote
title_short Filtered potential unreal huge proteins [Dataset]
title_full Filtered potential unreal huge proteins [Dataset]
title_fullStr Filtered potential unreal huge proteins [Dataset]
title_full_unstemmed Filtered potential unreal huge proteins [Dataset]
title_sort Filtered potential unreal huge proteins [Dataset]
dc.creator.none.fl_str_mv Amaral, Anibal S.
Devos, Damien P.
author Amaral, Anibal S.
author_facet Amaral, Anibal S.
Devos, Damien P.
author_role author
author2 Devos, Damien P.
author2_role author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Protein length distribution
5000 amino acids
Ribosomomal peptide synthesis
div >< p
Recognition surface proteins
Key cellular processes
Proteomes
Polyketide synthesis
Planctomycetota </
Elusimicrobiota </
Ubiquitin ligases
Potential impact
Overlooked aspect
Neglected giants
Majority found
Huge proteins
Host attachment
Homologous sequences
Highest propensity
Higher tendency
Functions related
Functional groups
Findings underscore
Examining uniprotkb
Ecological roles
Cytoskeleton organization
Average eukaryote
topic Protein length distribution
5000 amino acids
Ribosomomal peptide synthesis
div >< p
Recognition surface proteins
Key cellular processes
Proteomes
Polyketide synthesis
Planctomycetota </
Elusimicrobiota </
Ubiquitin ligases
Potential impact
Overlooked aspect
Neglected giants
Majority found
Huge proteins
Host attachment
Homologous sequences
Highest propensity
Higher tendency
Functions related
Functional groups
Findings underscore
Examining uniprotkb
Ecological roles
Cytoskeleton organization
Average eukaryote
description An often-overlooked aspect of biology is formed by the outliers of the protein length distribution, specifically those proteins with more than 5000 amino acids, which we refer to as huge proteins (HPs). By examining UniprotKB, we discovered more than 41 000 HPs throughout the tree of life, with the majority found in eukaryotes. Notably, the phyla with the highest propensity for HPs are Apicomplexa and Fornicata. Moreover, we observed that certain bacteria, such as Elusimicrobiota or Planctomycetota, have a higher tendency for encoding HPs, even more than the average eukaryote. To investigate if these macro-polypeptides represent “real” proteins, we explored several indirect metrics. Additionally, orthology analyses reveals thousands of clusters of homologous sequences of HPs, revealing functional groups related to key cellular processes such as cytoskeleton organization and functioning as chaperones or as E3-ubiquitin ligases in eukaryotes. In the case of bacteria, the major clusters have functions related to non-ribosomomal peptide synthesis/polyketide synthesis, followed by pathogen-host attachment or recognition surface proteins. Further exploration of the annotations for each HPs supported the previously identified functional groups. These findings underscore the need for further investigation of the cellular and ecological roles of these HPs and their potential impact on biology and biotechnology.
publishDate 2024
dc.date.none.fl_str_mv 2024
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/dataset
http://purl.org/coar/resource_type/c_ddb1
Publisher's version
info:eu-repo/semantics/publishedVersion
format dataset
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/385326
url http://hdl.handle.net/10261/385326
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Amaral, Anibal S.; Devos, Damien P. The neglected giants: Uncovering the prevalence and functional groups of huge proteins in proteomes. https://doi.org/10.1371/journal.pcbi.1012459 . http://hdl.handle.net/10261/379969
https://doi.org/10.1371/journal.pcbi.1012459.s007
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869409267034882048
score 15,812455

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