Small RNA-based antiviral defense in the phytopathogenic fungus Colletotrichum higginsianum

Even though the fungal kingdom contains more than 3 million species, little is known about the biological roles of RNA silencing in fungi. The Colletotrichum genus comprises fungal species that are pathogenic for a wide range of crop species worldwide. To investigate the role of RNA silencing in the...

Descripción completa

Detalles Bibliográficos
Autores: Campo Sánchez, Sonia|||0000-0001-5477-3162, Gilbert, Kerrigan B., Carrington, James C.
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/447027
Acceso en línea:https://hdl.handle.net/2117/447027
https://dx.doi.org/10.1371/journal.ppat.1005640
Access Level:acceso abierto
Palabra clave:Àrees temàtiques de la UPC::Enginyeria agroalimentària::Agricultura::Fitopatologia
Àrees temàtiques de la UPC::Enginyeria agroalimentària::Agricultura::Biotecnologia i millora genètica vegetal
id ES_600abbac751aa6d2cbc1ab5d7e3f762f
oai_identifier_str oai:upcommons.upc.edu:2117/447027
network_acronym_str ES
network_name_str España
repository_id_str
spelling Small RNA-based antiviral defense in the phytopathogenic fungus Colletotrichum higginsianumCampo Sánchez, Sonia|||0000-0001-5477-3162Gilbert, Kerrigan B.Carrington, James C.Àrees temàtiques de la UPC::Enginyeria agroalimentària::Agricultura::FitopatologiaÀrees temàtiques de la UPC::Enginyeria agroalimentària::Agricultura::Biotecnologia i millora genètica vegetalEven though the fungal kingdom contains more than 3 million species, little is known about the biological roles of RNA silencing in fungi. The Colletotrichum genus comprises fungal species that are pathogenic for a wide range of crop species worldwide. To investigate the role of RNA silencing in the ascomycete fungus Colletotrichum higginsianum, knock-out mutants affecting genes for three RNA-dependent RNA polymerase (RDR), two Dicer-like (DCL), and two Argonaute (AGO) proteins were generated by targeted gene replacement. No effects were observed on vegetative growth for any mutant strain when grown on complex or minimal media. However, ¿dcl1, ¿dcl1¿dcl2 double mutant, and ¿ago1 strains showed severe defects in conidiation and conidia morphology. Total RNA transcripts and small RNA populations were analyzed in parental and mutant strains. The greatest effects on both RNA populations was observed in the ¿dcl1, ¿dcl1¿dcl2, and ¿ago1 strains, in which a previously uncharacterized dsRNA mycovirus [termed Colletotrichum higginsianum non-segmented dsRNA virus 1 (ChNRV1)] was derepressed. Phylogenetic analyses clearly showed a close relationship between ChNRV1 and members of the segmented Partitiviridae family, despite the non-segmented nature of the genome. Immunoprecipitation of small RNAs associated with AGO1 showed abundant loading of 5’U-containing viral siRNA. C. higginsianum parental and ¿dcl1 mutant strains cured of ChNRV1 revealed that the conidiation and spore morphology defects were primarily caused by ChNRV1. Based on these results, RNA silencing involving ChDCL1 and ChAGO1 in C. higginsianum is proposed to function as an antiviral mechanism.20162016-06-0220252025-11-25journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/447027https://dx.doi.org/10.1371/journal.ppat.1005640reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/4470272026-05-27T15:37:01Z
dc.title.none.fl_str_mv Small RNA-based antiviral defense in the phytopathogenic fungus Colletotrichum higginsianum
title Small RNA-based antiviral defense in the phytopathogenic fungus Colletotrichum higginsianum
spellingShingle Small RNA-based antiviral defense in the phytopathogenic fungus Colletotrichum higginsianum
Campo Sánchez, Sonia|||0000-0001-5477-3162
Àrees temàtiques de la UPC::Enginyeria agroalimentària::Agricultura::Fitopatologia
Àrees temàtiques de la UPC::Enginyeria agroalimentària::Agricultura::Biotecnologia i millora genètica vegetal
title_short Small RNA-based antiviral defense in the phytopathogenic fungus Colletotrichum higginsianum
title_full Small RNA-based antiviral defense in the phytopathogenic fungus Colletotrichum higginsianum
title_fullStr Small RNA-based antiviral defense in the phytopathogenic fungus Colletotrichum higginsianum
title_full_unstemmed Small RNA-based antiviral defense in the phytopathogenic fungus Colletotrichum higginsianum
title_sort Small RNA-based antiviral defense in the phytopathogenic fungus Colletotrichum higginsianum
dc.creator.none.fl_str_mv Campo Sánchez, Sonia|||0000-0001-5477-3162
Gilbert, Kerrigan B.
Carrington, James C.
author Campo Sánchez, Sonia|||0000-0001-5477-3162
author_facet Campo Sánchez, Sonia|||0000-0001-5477-3162
Gilbert, Kerrigan B.
Carrington, James C.
author_role author
author2 Gilbert, Kerrigan B.
Carrington, James C.
author2_role author
author
dc.subject.none.fl_str_mv Àrees temàtiques de la UPC::Enginyeria agroalimentària::Agricultura::Fitopatologia
Àrees temàtiques de la UPC::Enginyeria agroalimentària::Agricultura::Biotecnologia i millora genètica vegetal
topic Àrees temàtiques de la UPC::Enginyeria agroalimentària::Agricultura::Fitopatologia
Àrees temàtiques de la UPC::Enginyeria agroalimentària::Agricultura::Biotecnologia i millora genètica vegetal
description Even though the fungal kingdom contains more than 3 million species, little is known about the biological roles of RNA silencing in fungi. The Colletotrichum genus comprises fungal species that are pathogenic for a wide range of crop species worldwide. To investigate the role of RNA silencing in the ascomycete fungus Colletotrichum higginsianum, knock-out mutants affecting genes for three RNA-dependent RNA polymerase (RDR), two Dicer-like (DCL), and two Argonaute (AGO) proteins were generated by targeted gene replacement. No effects were observed on vegetative growth for any mutant strain when grown on complex or minimal media. However, ¿dcl1, ¿dcl1¿dcl2 double mutant, and ¿ago1 strains showed severe defects in conidiation and conidia morphology. Total RNA transcripts and small RNA populations were analyzed in parental and mutant strains. The greatest effects on both RNA populations was observed in the ¿dcl1, ¿dcl1¿dcl2, and ¿ago1 strains, in which a previously uncharacterized dsRNA mycovirus [termed Colletotrichum higginsianum non-segmented dsRNA virus 1 (ChNRV1)] was derepressed. Phylogenetic analyses clearly showed a close relationship between ChNRV1 and members of the segmented Partitiviridae family, despite the non-segmented nature of the genome. Immunoprecipitation of small RNAs associated with AGO1 showed abundant loading of 5’U-containing viral siRNA. C. higginsianum parental and ¿dcl1 mutant strains cured of ChNRV1 revealed that the conidiation and spore morphology defects were primarily caused by ChNRV1. Based on these results, RNA silencing involving ChDCL1 and ChAGO1 in C. higginsianum is proposed to function as an antiviral mechanism.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-06-02
2025
2025-11-25
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/447027
https://dx.doi.org/10.1371/journal.ppat.1005640
url https://hdl.handle.net/2117/447027
https://dx.doi.org/10.1371/journal.ppat.1005640
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869409263552561152
score 15.81155

Similares en LA Referencia