A complex regulatory network governs the expression of symbiotic genes in Sinorhizobium fredii HH103

[Introduction]: The establishment of the rhizobium-legume nitrogen-fixing symbiosis relies on the interchange of molecular signals between the two symbionts. We have previously studied by RNA-seq the effect of the symbiotic regulators NodD1, SyrM, and TtsI on the expression of the symbiotic genes (t...

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Bibliographic Details
Authors: Navarro-Gómez, Pilar, Fuentes-Romero, Francisco, Pérez-Montaño, Francisco, Jiménez-Guerrero, Irene, Alias-Villegas, C., Ayala-García, P., Almozara, Andrés, Medina, Carlos, Ollero, Francisco Javier, Rodríguez-Carvajal, Miguel Ángel, Ruiz-Sainz, José-Enrique, López-Baena, Francisco Javier, Vinardell, José-María, Acosta-Jurado, Sebastián
Format: article
Status:Published version
Publication Date:2023
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/351772
Online Access:http://hdl.handle.net/10261/351772
Access Level:Open access
Keyword:Rhizobium-legume symbiosis
Nod factors
NodD1
NodD2
NolR
SyrM
TtsI
Symbiotic genes regulatory network
Description
Summary:[Introduction]: The establishment of the rhizobium-legume nitrogen-fixing symbiosis relies on the interchange of molecular signals between the two symbionts. We have previously studied by RNA-seq the effect of the symbiotic regulators NodD1, SyrM, and TtsI on the expression of the symbiotic genes (the nod regulon) of Sinorhizobium fredii HH103 upon treatment with the isoflavone genistein. In this work we have further investigated this regulatory network by incorporating new RNA-seq data of HH103 mutants in two other regulatory genes, nodD2 and nolR. Both genes code for global regulators with a predominant repressor effect on the nod regulon, although NodD2 acts as an activator of a small number of HH103 symbiotic genes.

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