Molecular identification and characterization of haptoglobin in teleosts revealed an important role on fish viral infections

Haptoglobin (Hp) molecule has been cloned and characterized in two marine teleosts (gilthead seabream and European sea bass), obtaining putative proteins of 319 residues encoded by an ORF of 960 bp in both species. However, the matrix of similarity revealed low identities among bony fish species 78....

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Detalles Bibliográficos
Autores: Cordero, Héctor, Hong Li, Chang, Chaves-Pozo, Elena, Esteban, María Ángeles, Cuesta, Alberto
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/313454
Acceso en línea:http://hdl.handle.net/10261/313454
Access Level:acceso abierto
Palabra clave:Haptoglobin
Acuicultura
Centro Oceanográfico de Murcia
nodavirus
LCDV
teleost
Gilthead seabream
European sea bass
fish
cytology
marine sciences
histology
molecular biology
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spelling Molecular identification and characterization of haptoglobin in teleosts revealed an important role on fish viral infectionsCordero, HéctorHong Li, ChangChaves-Pozo, ElenaEsteban, María ÁngelesCuesta, AlbertoHaptoglobinAcuiculturaCentro Oceanográfico de MurcianodavirusLCDVteleostGilthead seabreamEuropean sea bassfishcytologymarine scienceshistologymolecular biologyHaptoglobin (Hp) molecule has been cloned and characterized in two marine teleosts (gilthead seabream and European sea bass), obtaining putative proteins of 319 residues encoded by an ORF of 960 bp in both species. However, the matrix of similarity revealed low identities among bony fish species 78.9% (seabream-sea bass), 43% (seabream/seabass-zebrafish) and lower than 20% with sharks and human. The protein sequences showed a signal peptide from the position 1 to 23, a trypsin domain from 47 to 297, and several predicted disulfide bridges and glycosylation sites. The expression of hp transcript levels during ontogeny showed a progressive increase of expression in seabream whilst remained almost unaltered in sea bass. By tissues, this gene was found constitutively expressed with the highest levels on liver in both species. The main results on hp transcript levels showed the up-regulation in gilthead seabream suffering from naturally occurring lymphocystis disease; and the down-regulation and up-regulation after nodavirus infection in the resistant gilthead seabream and the susceptible European sea bass, respectively. These findings demonstrate for the first time an important role of haptoglobin against viral infections, operating differently in two of the most important marine farmed fish species.Dr. H. Cordero is grateful to the Spanish Ministry of Economy and Competitiveness (MINECO) for a grant (BES-2012-052742). This work was co-funded by several national projects of the MINECO and MINECO-FEDER (grant numbers AGL2011-30381-C03-01, AGL2013-43588-P and AGL2014-51839-C5-1-R) and Fundación Séneca de la Región de Murcia (Grupo de Excelencia grant number 19883/GERM/15).Peer reviewedMinisterio de Economía y Competitividad (España)Fundación SénecaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/313454reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//BES-2012-052742info:eu-repo/grantAgreement/MICINN//AGL2011-30381-C03-01info:eu-repo/grantAgreement/MINECO//AGL2013-43588-PCentro Oceanográfico de Murciahttps://doi.org/10.1016/j.dci.2017.06.006https://doi.org/10.1016/j.dci.2017.06.006Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3134542026-05-22T06:33:51Z
dc.title.none.fl_str_mv Molecular identification and characterization of haptoglobin in teleosts revealed an important role on fish viral infections
title Molecular identification and characterization of haptoglobin in teleosts revealed an important role on fish viral infections
spellingShingle Molecular identification and characterization of haptoglobin in teleosts revealed an important role on fish viral infections
Cordero, Héctor
Haptoglobin
Acuicultura
Centro Oceanográfico de Murcia
nodavirus
LCDV
teleost
Gilthead seabream
European sea bass
fish
cytology
marine sciences
histology
molecular biology
title_short Molecular identification and characterization of haptoglobin in teleosts revealed an important role on fish viral infections
title_full Molecular identification and characterization of haptoglobin in teleosts revealed an important role on fish viral infections
title_fullStr Molecular identification and characterization of haptoglobin in teleosts revealed an important role on fish viral infections
title_full_unstemmed Molecular identification and characterization of haptoglobin in teleosts revealed an important role on fish viral infections
title_sort Molecular identification and characterization of haptoglobin in teleosts revealed an important role on fish viral infections
dc.creator.none.fl_str_mv Cordero, Héctor
Hong Li, Chang
Chaves-Pozo, Elena
Esteban, María Ángeles
Cuesta, Alberto
author Cordero, Héctor
author_facet Cordero, Héctor
Hong Li, Chang
Chaves-Pozo, Elena
Esteban, María Ángeles
Cuesta, Alberto
author_role author
author2 Hong Li, Chang
Chaves-Pozo, Elena
Esteban, María Ángeles
Cuesta, Alberto
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Fundación Séneca
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Haptoglobin
Acuicultura
Centro Oceanográfico de Murcia
nodavirus
LCDV
teleost
Gilthead seabream
European sea bass
fish
cytology
marine sciences
histology
molecular biology
topic Haptoglobin
Acuicultura
Centro Oceanográfico de Murcia
nodavirus
LCDV
teleost
Gilthead seabream
European sea bass
fish
cytology
marine sciences
histology
molecular biology
description Haptoglobin (Hp) molecule has been cloned and characterized in two marine teleosts (gilthead seabream and European sea bass), obtaining putative proteins of 319 residues encoded by an ORF of 960 bp in both species. However, the matrix of similarity revealed low identities among bony fish species 78.9% (seabream-sea bass), 43% (seabream/seabass-zebrafish) and lower than 20% with sharks and human. The protein sequences showed a signal peptide from the position 1 to 23, a trypsin domain from 47 to 297, and several predicted disulfide bridges and glycosylation sites. The expression of hp transcript levels during ontogeny showed a progressive increase of expression in seabream whilst remained almost unaltered in sea bass. By tissues, this gene was found constitutively expressed with the highest levels on liver in both species. The main results on hp transcript levels showed the up-regulation in gilthead seabream suffering from naturally occurring lymphocystis disease; and the down-regulation and up-regulation after nodavirus infection in the resistant gilthead seabream and the susceptible European sea bass, respectively. These findings demonstrate for the first time an important role of haptoglobin against viral infections, operating differently in two of the most important marine farmed fish species.
publishDate 2017
dc.date.none.fl_str_mv 2017
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/313454
url http://hdl.handle.net/10261/313454
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO//BES-2012-052742
info:eu-repo/grantAgreement/MICINN//AGL2011-30381-C03-01
info:eu-repo/grantAgreement/MINECO//AGL2013-43588-P
Centro Oceanográfico de Murcia
https://doi.org/10.1016/j.dci.2017.06.006
https://doi.org/10.1016/j.dci.2017.06.006
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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