Mitotic biology of primary neurons

Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 22-11-2018

Bibliographic Details
Author: Walton Enríquez, Chaska Carlos
Format: doctoral thesis
Publication Date:2018
Country:España
Institution:Universidad Autónoma de Madrid
Repository:Biblos-e Archivo. Repositorio Institucional de la UAM
Language:English
OAI Identifier:oai:repositorio.uam.es:10486/686236
Online Access:http://hdl.handle.net/10486/686236
Access Level:Open access
Keyword:Neuronas - Tesis doctorales
Biología y Biomedicina / Biología
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spelling Mitotic biology of primary neuronsWalton Enríquez, Chaska CarlosNeuronas - Tesis doctoralesBiología y Biomedicina / BiologíaTesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 22-11-2018Cell cycle regulators are expressed in neurons but they carry out non-cell cycle functions involving maturation, migration and synapse regulation. Neurons are nevertheless reported to re-enter the cell cycle in neurodegenerative diseases such as Alzheimer’s disease (AD) and Parkinson’s disease (PD). Neuronal cell cycle re-entry is also well established in in vivo and in vitro rodent models. However, cell cycle re-entry is usually associated to cell death instead of cell division. Thus, it is largely believed the cell cycle machinery of neurons is not fully functional, which is generally considered to be a consequence of their postmitotic status. Notwithstanding, we lack proof that the cell cycle machinery of neurons is dysfunctional nor whether its regulation is different from that of mitotic cells. The objective of the present work is to test the extent to which neurons can progress through the cell cycle and whether neurons and mitotic cells share the regulation of the cell cycle. To do so, cell cycle re-entry in mature primary hippocampal neurons was induced by Cyclin E-Cdk2 fusion products. The fusion products induce cell cycle re-entry, cell cycle deregulation and, as in mitotic cells, this results in apoptosis instead of cell division. Howbeit, abrogation of checkpoint signaling in S, G2 or M-phases enables primary neurons to progress through the cell cycle. Neurons can readily enter M-phase and a subset of neurons undergoes cell division. Moreover, neurons adapt their differentiated biology to the cell cycle. In M-phase they lose the integrity of the axon initial segment (AIS), which is necessary to integrate synaptic input and generate action potentials as well as for neuronal viability. After prematurely exiting M-phase without undergoing cell division neurons recover the AIS. In conclusion, neurons and mitotic cells share G1, S, G2 and M-phase regulation and are capable of undergoing cell divisionFrade López, José MaríaPorlán Alonso, EvaDepartamento de Biología MolecularFacultad de CienciasCSIC. Instituto Cajal (IC)20182018-11-22doctoral thesishttp://purl.org/coar/resource_type/c_db06NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/doctoralThesisapplication/pdfhttp://hdl.handle.net/10486/686236reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/6862362026-06-23T12:46:27Z
dc.title.none.fl_str_mv Mitotic biology of primary neurons
title Mitotic biology of primary neurons
spellingShingle Mitotic biology of primary neurons
Walton Enríquez, Chaska Carlos
Neuronas - Tesis doctorales
Biología y Biomedicina / Biología
title_short Mitotic biology of primary neurons
title_full Mitotic biology of primary neurons
title_fullStr Mitotic biology of primary neurons
title_full_unstemmed Mitotic biology of primary neurons
title_sort Mitotic biology of primary neurons
dc.creator.none.fl_str_mv Walton Enríquez, Chaska Carlos
author Walton Enríquez, Chaska Carlos
author_facet Walton Enríquez, Chaska Carlos
author_role author
dc.contributor.none.fl_str_mv Frade López, José María
Porlán Alonso, Eva
Departamento de Biología Molecular
Facultad de Ciencias
CSIC. Instituto Cajal (IC)
dc.subject.none.fl_str_mv Neuronas - Tesis doctorales
Biología y Biomedicina / Biología
topic Neuronas - Tesis doctorales
Biología y Biomedicina / Biología
description Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 22-11-2018
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-11-22
dc.type.none.fl_str_mv doctoral thesis
http://purl.org/coar/resource_type/c_db06
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/686236
url http://hdl.handle.net/10486/686236
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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