A Color indicator based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and a biodegradable poly(ester amide) for detecting bacterial contamination

Bacterial contamination is a hazard in many industries, including food, pharmaceuticals, and healthcare. The availability of a rapid and simple method for detecting this type of contamination in sterile areas enables immediate intervention to avoid or reduce detrimental effects. Among these methods,...

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Autores: Lovato Vélez, María José, Lama Odría, María del Carmen Elizabeth de|||0000-0002-5076-8312, Puiggalí Bellalta, Jordi|||0000-0002-0640-4474, Valle Mendoza, Luis Javier del|||0000-0001-9916-1741, Franco García, María Lourdes|||0000-0001-5968-285X
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/411768
Acceso en línea:https://hdl.handle.net/2117/411768
https://dx.doi.org/10.3390/ijms25126671
Access Level:acceso abierto
Palabra clave:Polymers -- Biodegradation
Electrospinning
Color indicator
MTT
Bacteria
Biodegradable
Poly(ester amide)
Polímers -- Biodegradació
Àrees temàtiques de la UPC::Enginyeria química
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spelling A Color indicator based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and a biodegradable poly(ester amide) for detecting bacterial contaminationLovato Vélez, María JoséLama Odría, María del Carmen Elizabeth de|||0000-0002-5076-8312Puiggalí Bellalta, Jordi|||0000-0002-0640-4474Valle Mendoza, Luis Javier del|||0000-0001-9916-1741Franco García, María Lourdes|||0000-0001-5968-285XPolymers -- BiodegradationElectrospinningColor indicatorMTTBacteriaBiodegradablePoly(ester amide)ElectrospinningPolímers -- BiodegradacióÀrees temàtiques de la UPC::Enginyeria químicaBacterial contamination is a hazard in many industries, including food, pharmaceuticals, and healthcare. The availability of a rapid and simple method for detecting this type of contamination in sterile areas enables immediate intervention to avoid or reduce detrimental effects. Among these methods, colorimetric indicators are becoming increasingly popular due to their affordability, ease of use, and quick visual interpretation of the signal. In this article, a bacterial contamination indicator system was designed by incorporating MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) into an electrospun PADAS matrix, which is a biodegradable poly(ester amide) synthesized from L-alanine, 1,12-dodecanediol, and sebacic acid. Uniaxial stress testing, thermogravimetric analysis and scanning electron microscopy were used to examine the mechanical properties, thermal stability, and morphology of the mats, respectively. The capacity for bacterial detection was not only analyzed with agar and broth assays but also by replicating important environmental conditions. Among the MTT concentrations tested in this study (0.2%, 2%, and 5%), it was found that only with a 2% MTT content the designed system produced a color response visible to the naked eye with optimal intensity, a sensitivity limit of 104 CFU/mL, and 86% cell viability, which showed the great potential for its use to detect bacterial contamination. In summary, by means of the process described in this work, it was possible to obtain a simple, low-cost and fast-response bacterial contamination indicator that can be used in mask filters, air filters, or protective clothing.This research was funded by the Spanish Ministry of Science, Innovation and Universities through PID2022-140302OB-I00 and by the Generalitat de Catalunya under the project 2021-SGR-01042.Peer ReviewedMultidisciplinary Digital Publishing Institute (MDPI)20242024-06-1820242024-07-16journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/411768https://dx.doi.org/10.3390/ijms25126671reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)InglésengAgencia Estatal de Investigación http://doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2022-140302OB-I00 COPOLIMEROS DE 3-HIDROXIBUTIRATO Y 3-HIDROXIVALERATO A PARTIR DE RESIDUOS INDUSTRIALES: MODULACION, NANOTEXTURIZACION, MICROMOLDEADO POR ULTRASONIDOS Y APLICACIONESopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/4117682026-05-27T15:37:01Z
dc.title.none.fl_str_mv A Color indicator based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and a biodegradable poly(ester amide) for detecting bacterial contamination
title A Color indicator based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and a biodegradable poly(ester amide) for detecting bacterial contamination
spellingShingle A Color indicator based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and a biodegradable poly(ester amide) for detecting bacterial contamination
Lovato Vélez, María José
Polymers -- Biodegradation
Electrospinning
Color indicator
MTT
Bacteria
Biodegradable
Poly(ester amide)
Electrospinning
Polímers -- Biodegradació
Àrees temàtiques de la UPC::Enginyeria química
title_short A Color indicator based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and a biodegradable poly(ester amide) for detecting bacterial contamination
title_full A Color indicator based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and a biodegradable poly(ester amide) for detecting bacterial contamination
title_fullStr A Color indicator based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and a biodegradable poly(ester amide) for detecting bacterial contamination
title_full_unstemmed A Color indicator based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and a biodegradable poly(ester amide) for detecting bacterial contamination
title_sort A Color indicator based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and a biodegradable poly(ester amide) for detecting bacterial contamination
dc.creator.none.fl_str_mv Lovato Vélez, María José
Lama Odría, María del Carmen Elizabeth de|||0000-0002-5076-8312
Puiggalí Bellalta, Jordi|||0000-0002-0640-4474
Valle Mendoza, Luis Javier del|||0000-0001-9916-1741
Franco García, María Lourdes|||0000-0001-5968-285X
author Lovato Vélez, María José
author_facet Lovato Vélez, María José
Lama Odría, María del Carmen Elizabeth de|||0000-0002-5076-8312
Puiggalí Bellalta, Jordi|||0000-0002-0640-4474
Valle Mendoza, Luis Javier del|||0000-0001-9916-1741
Franco García, María Lourdes|||0000-0001-5968-285X
author_role author
author2 Lama Odría, María del Carmen Elizabeth de|||0000-0002-5076-8312
Puiggalí Bellalta, Jordi|||0000-0002-0640-4474
Valle Mendoza, Luis Javier del|||0000-0001-9916-1741
Franco García, María Lourdes|||0000-0001-5968-285X
author2_role author
author
author
author
dc.subject.none.fl_str_mv Polymers -- Biodegradation
Electrospinning
Color indicator
MTT
Bacteria
Biodegradable
Poly(ester amide)
Electrospinning
Polímers -- Biodegradació
Àrees temàtiques de la UPC::Enginyeria química
topic Polymers -- Biodegradation
Electrospinning
Color indicator
MTT
Bacteria
Biodegradable
Poly(ester amide)
Electrospinning
Polímers -- Biodegradació
Àrees temàtiques de la UPC::Enginyeria química
description Bacterial contamination is a hazard in many industries, including food, pharmaceuticals, and healthcare. The availability of a rapid and simple method for detecting this type of contamination in sterile areas enables immediate intervention to avoid or reduce detrimental effects. Among these methods, colorimetric indicators are becoming increasingly popular due to their affordability, ease of use, and quick visual interpretation of the signal. In this article, a bacterial contamination indicator system was designed by incorporating MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) into an electrospun PADAS matrix, which is a biodegradable poly(ester amide) synthesized from L-alanine, 1,12-dodecanediol, and sebacic acid. Uniaxial stress testing, thermogravimetric analysis and scanning electron microscopy were used to examine the mechanical properties, thermal stability, and morphology of the mats, respectively. The capacity for bacterial detection was not only analyzed with agar and broth assays but also by replicating important environmental conditions. Among the MTT concentrations tested in this study (0.2%, 2%, and 5%), it was found that only with a 2% MTT content the designed system produced a color response visible to the naked eye with optimal intensity, a sensitivity limit of 104 CFU/mL, and 86% cell viability, which showed the great potential for its use to detect bacterial contamination. In summary, by means of the process described in this work, it was possible to obtain a simple, low-cost and fast-response bacterial contamination indicator that can be used in mask filters, air filters, or protective clothing.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-06-18
2024
2024-07-16
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/411768
https://dx.doi.org/10.3390/ijms25126671
url https://hdl.handle.net/2117/411768
https://dx.doi.org/10.3390/ijms25126671
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2022-140302OB-I00 COPOLIMEROS DE 3-HIDROXIBUTIRATO Y 3-HIDROXIVALERATO A PARTIR DE RESIDUOS INDUSTRIALES: MODULACION, NANOTEXTURIZACION, MICROMOLDEADO POR ULTRASONIDOS Y APLICACIONES
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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