Neuroprotective Effect of 3',4'-Dihydroxyphenylglycol in Type-1-like Diabetic Rats-Influence of the Hydroxytyrosol/3',4'-dihydroxyphenylglycol Ratio.

The aim of this study was to assess the possible neuroprotective effect of 3',4'-dihydroxyphenylglycol (DHPG), a polyphenol from extra virgin olive oil (EVOO), in an experimental model of diabetes and whether this effect is modified by the presence of another EVOO polyphenol, hydroxytyroso...

ver descrição completa

Detalhes bibliográficos
Autores: Rodríguez-Pérez, María Dolores, Pérez de Algaba, Inmaculada, Martín-Aurioles, Esther, Arrebola, María Monsalud, Ortega-Hombrados, Laura, Verdugo, Cristina, Fernández-Prior, María África, Bermúdez-Oria, Alejandra, De La Cruz, José Pedro, González-Correa, José Antonio
Formato: artículo
Fecha de publicación:2022
País:España
Recursos:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/18773
Acesso em linha:http://hdl.handle.net/20.500.12105/18773
Access Level:acceso abierto
Palavra-chave:3′,4′-dihydroxyphenylglycol
diabetes mellitus
diabetic neuropathy
extra virgin olive oil
hydroxytyrosol
Animals
Diabetes Mellitus, Experimental
Neuroprotective Agents
Phenylethyl Alcohol
Rats
Rats, Wistar
Descrição
Resumo:The aim of this study was to assess the possible neuroprotective effect of 3',4'-dihydroxyphenylglycol (DHPG), a polyphenol from extra virgin olive oil (EVOO), in an experimental model of diabetes and whether this effect is modified by the presence of another EVOO polyphenol, hydroxytyrosol (HT). The neuroprotective effect was assessed in a hypoxia-reoxygenation model in brain slices and by quantifying retinal nerve cells. The animals were distributed as follows: (1) normoglycemic rats (NDR), (2) diabetic rats (DR), (3) DR treated with HT (5 mg/kg/day p.o.), (4) DR treated with DHPG (0.5 mg/kg/day), or (5) with 1 mg/kg/day, (6) DR treated with HT plus DHPG 0.5 mg/kg/day, or (7) HT plus 1 mg/kg/day p.o. DHPG. Diabetic animals presented higher levels of oxidative stress variables and lower numbers of neuronal cells in retinal tissue. The administration of DHPG or HT reduced most of the oxidative stress variables and brain lactate dehydrogenase efflux (LDH) as an indirect index of cellular death and also reduced the loss of retinal cells. The association of DHPG+HT in the same proportions, as found in EVOO, improved the neuroprotective and antioxidant effects of both polyphenols.

Registos relacionados