Somatic embryogenesis in Alnus glutinosa (L.) Gaertn

Induction of somatic embryos and plant regeneration was demonstrated for the first time in Alnus glutinosa. Somatic embryos were initiated from zygotic embryos collected 1–3 weeks post-anthesis (WPA), i.e., when they were at globular or early cotyledonary stage and were 0.5–1 mm in length. Induction...

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Detalhes bibliográficos
Autores: Corredoira, Elena, Valladares, Silvia, Martínez-Santiago, María Teresa, Viéitez Martín, Ana María, San José, M. Carmen
Formato: artículo
Estado:Versión enviada para evaluación y publicación
Fecha de publicación:2013
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/196911
Acesso em linha:http://hdl.handle.net/10261/196911
Access Level:acceso abierto
Palavra-chave:Black alder
Cones
Histology
Secondary embryogenesis
Somatic embryos
Zygotic embryos
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spelling Somatic embryogenesis in Alnus glutinosa (L.) GaertnCorredoira, ElenaValladares, SilviaMartínez-Santiago, María TeresaViéitez Martín, Ana MaríaSan José, M. CarmenBlack alderConesHistologySecondary embryogenesisSomatic embryosZygotic embryosInduction of somatic embryos and plant regeneration was demonstrated for the first time in Alnus glutinosa. Somatic embryos were initiated from zygotic embryos collected 1–3 weeks post-anthesis (WPA), i.e., when they were at globular or early cotyledonary stage and were 0.5–1 mm in length. Induction frequency (16.6 %) and the mean number of somatic embryos (4.5 embryos/explant) were highest after culture of zygotic embryos, collected at 3 WPA, on Murashige and Skoog medium (MS) supplemented with 0.9-μM 2,4-dichlorophenoxyacetic acid and 2.22-μM benzyladenine (BA). No embryogenic induction was observed on medium with BA alone. Initial somatic embryos differentiated indirectly from callus tissue formed at the surface of the zygotic embryos. Embryogenic competence was maintained by secondary embryogenesis, which was affected by explant type, plant growth regulators and genotype. Secondary embryogenesis was induced by culture of small groups of whole somatic embryos or isolated cotyledon explants on medium consisting of MS medium (half-strength macronutrients) supplemented with 0.44-μM BA. Histological study of isolated cotyledon explants revealed that secondary embryos developed directly from differentiated embryogenic tissue on the surface of cotyledons. Somatic embryos at successive stages of development, including cotyledonary-stage embryos with shoot and root meristems, were evident. For plantlet conversion, somatic embryos were transferred to maturation medium supplemented with 3 % maltose, followed by 6 weeks of culture in Woody Plant Medium supplemented with 0.44-μM BA and 0.46-μM Zeatin (Z). This novel protocol appears promising for mass propagation, conservation and genetic transformation of black alder.This research was partially funded by INLUDES (Diputacion Provincial de Lugo) and Xunta de Galicia (Spain) through Project 09MRU002400PR.Peer reviewedSpringer NatureDiputación Provincial de LugoXunta de GaliciaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192013info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Preprintinfo:eu-repo/semantics/submittedVersionhttp://hdl.handle.net/10261/196911reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1007/s00468-013-0907-8Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1969112026-05-22T06:33:51Z
dc.title.none.fl_str_mv Somatic embryogenesis in Alnus glutinosa (L.) Gaertn
title Somatic embryogenesis in Alnus glutinosa (L.) Gaertn
spellingShingle Somatic embryogenesis in Alnus glutinosa (L.) Gaertn
Corredoira, Elena
Black alder
Cones
Histology
Secondary embryogenesis
Somatic embryos
Zygotic embryos
title_short Somatic embryogenesis in Alnus glutinosa (L.) Gaertn
title_full Somatic embryogenesis in Alnus glutinosa (L.) Gaertn
title_fullStr Somatic embryogenesis in Alnus glutinosa (L.) Gaertn
title_full_unstemmed Somatic embryogenesis in Alnus glutinosa (L.) Gaertn
title_sort Somatic embryogenesis in Alnus glutinosa (L.) Gaertn
dc.creator.none.fl_str_mv Corredoira, Elena
Valladares, Silvia
Martínez-Santiago, María Teresa
Viéitez Martín, Ana María
San José, M. Carmen
author Corredoira, Elena
author_facet Corredoira, Elena
Valladares, Silvia
Martínez-Santiago, María Teresa
Viéitez Martín, Ana María
San José, M. Carmen
author_role author
author2 Valladares, Silvia
Martínez-Santiago, María Teresa
Viéitez Martín, Ana María
San José, M. Carmen
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Diputación Provincial de Lugo
Xunta de Galicia
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Black alder
Cones
Histology
Secondary embryogenesis
Somatic embryos
Zygotic embryos
topic Black alder
Cones
Histology
Secondary embryogenesis
Somatic embryos
Zygotic embryos
description Induction of somatic embryos and plant regeneration was demonstrated for the first time in Alnus glutinosa. Somatic embryos were initiated from zygotic embryos collected 1–3 weeks post-anthesis (WPA), i.e., when they were at globular or early cotyledonary stage and were 0.5–1 mm in length. Induction frequency (16.6 %) and the mean number of somatic embryos (4.5 embryos/explant) were highest after culture of zygotic embryos, collected at 3 WPA, on Murashige and Skoog medium (MS) supplemented with 0.9-μM 2,4-dichlorophenoxyacetic acid and 2.22-μM benzyladenine (BA). No embryogenic induction was observed on medium with BA alone. Initial somatic embryos differentiated indirectly from callus tissue formed at the surface of the zygotic embryos. Embryogenic competence was maintained by secondary embryogenesis, which was affected by explant type, plant growth regulators and genotype. Secondary embryogenesis was induced by culture of small groups of whole somatic embryos or isolated cotyledon explants on medium consisting of MS medium (half-strength macronutrients) supplemented with 0.44-μM BA. Histological study of isolated cotyledon explants revealed that secondary embryos developed directly from differentiated embryogenic tissue on the surface of cotyledons. Somatic embryos at successive stages of development, including cotyledonary-stage embryos with shoot and root meristems, were evident. For plantlet conversion, somatic embryos were transferred to maturation medium supplemented with 3 % maltose, followed by 6 weeks of culture in Woody Plant Medium supplemented with 0.44-μM BA and 0.46-μM Zeatin (Z). This novel protocol appears promising for mass propagation, conservation and genetic transformation of black alder.
publishDate 2013
dc.date.none.fl_str_mv 2013
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Preprint
info:eu-repo/semantics/submittedVersion
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/196911
url http://hdl.handle.net/10261/196911
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1007/s00468-013-0907-8
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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