The pH signaling transcription factor PacC is required for full virulence in Penicillium digitatum

Penicillium digitatum is the most important postharvest pathogen of citrus fruits. Along disease progression, the infected citrus peel tissue is acidified due to the accumulation of organic acids. So far, relatively little is known about the environmental factors that regulate pathogenicity in this...

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Autores: Zhang, T., Sun, X., Xu, Q., González-Candelas, Luis, Li, H.
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2013
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/127732
Acesso em linha:http://hdl.handle.net/10261/127732
Access Level:acceso abierto
Palavra-chave:Virulence
Transcription factor
pH signaling
Penicillium digitatum
Cell wall degradation enzyme genes
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spelling The pH signaling transcription factor PacC is required for full virulence in Penicillium digitatumZhang, T.Sun, X.Xu, Q.González-Candelas, LuisLi, H.VirulenceTranscription factorpH signalingPenicillium digitatumCell wall degradation enzyme genesPenicillium digitatum is the most important postharvest pathogen of citrus fruits. Along disease progression, the infected citrus peel tissue is acidified due to the accumulation of organic acids. So far, relatively little is known about the environmental factors that regulate pathogenicity in this fungus. In this study, the role of the pH signaling transcription factor PacC in the pathogenesis of P. digitatum was investigated. We identified the pacC ortholog (PdpacC) in P. digitatum and found that its transcript levels were elevated under alkaline conditions (pH ≥ 7) in vitro, as well as during the infection of citrus fruits in spite of the low pH (about 3.0 to 3.5) of the macerated tissue. Na+ and pectin also induced the expression of PdpacC. Disruption of PdpacC resulted in impaired mycelial growth under neutral or alkaline pH conditions and on synthetic medium supplemented with pectin as the sole carbon source, and attenuated virulence towards citrus fruits. Introducing the full length of PdpacC into the ΔPdpacC mutant restored all these phenotypes. The expression of the polygalacturonase gene Pdpg2 and pectin lyase gene Pdpnl1 in P. digitatum was upregulated in the wild type strain but not or weakly upregulated in the ΔPdpacC mutant during infection. Disruption of Pdpg2 also resulted in attenuated virulence of P. digitatum towards citrus fruits. Collectively, we conclude that PdPacC plays an important role in pathogenesis of P. digitatum via regulation of the expression of cell wall degradation enzyme genes, such as Pdpg2 and Pdpnl1.This work was supported by the National Foundation of Natural Science of China (31071649), China Agriculture Research System (CARS-27) and the Special Fund for Agro-scientific Research in the Public Interest (201203034).Peer ReviewedSpringer NatureNational Natural Science Foundation of ChinaAgro-scientific Research in the Public Interest (China)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2016201620132016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/127732reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1277322026-05-22T06:33:51Z
dc.title.none.fl_str_mv The pH signaling transcription factor PacC is required for full virulence in Penicillium digitatum
title The pH signaling transcription factor PacC is required for full virulence in Penicillium digitatum
spellingShingle The pH signaling transcription factor PacC is required for full virulence in Penicillium digitatum
Zhang, T.
Virulence
Transcription factor
pH signaling
Penicillium digitatum
Cell wall degradation enzyme genes
title_short The pH signaling transcription factor PacC is required for full virulence in Penicillium digitatum
title_full The pH signaling transcription factor PacC is required for full virulence in Penicillium digitatum
title_fullStr The pH signaling transcription factor PacC is required for full virulence in Penicillium digitatum
title_full_unstemmed The pH signaling transcription factor PacC is required for full virulence in Penicillium digitatum
title_sort The pH signaling transcription factor PacC is required for full virulence in Penicillium digitatum
dc.creator.none.fl_str_mv Zhang, T.
Sun, X.
Xu, Q.
González-Candelas, Luis
Li, H.
author Zhang, T.
author_facet Zhang, T.
Sun, X.
Xu, Q.
González-Candelas, Luis
Li, H.
author_role author
author2 Sun, X.
Xu, Q.
González-Candelas, Luis
Li, H.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv National Natural Science Foundation of China
Agro-scientific Research in the Public Interest (China)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Virulence
Transcription factor
pH signaling
Penicillium digitatum
Cell wall degradation enzyme genes
topic Virulence
Transcription factor
pH signaling
Penicillium digitatum
Cell wall degradation enzyme genes
description Penicillium digitatum is the most important postharvest pathogen of citrus fruits. Along disease progression, the infected citrus peel tissue is acidified due to the accumulation of organic acids. So far, relatively little is known about the environmental factors that regulate pathogenicity in this fungus. In this study, the role of the pH signaling transcription factor PacC in the pathogenesis of P. digitatum was investigated. We identified the pacC ortholog (PdpacC) in P. digitatum and found that its transcript levels were elevated under alkaline conditions (pH ≥ 7) in vitro, as well as during the infection of citrus fruits in spite of the low pH (about 3.0 to 3.5) of the macerated tissue. Na+ and pectin also induced the expression of PdpacC. Disruption of PdpacC resulted in impaired mycelial growth under neutral or alkaline pH conditions and on synthetic medium supplemented with pectin as the sole carbon source, and attenuated virulence towards citrus fruits. Introducing the full length of PdpacC into the ΔPdpacC mutant restored all these phenotypes. The expression of the polygalacturonase gene Pdpg2 and pectin lyase gene Pdpnl1 in P. digitatum was upregulated in the wild type strain but not or weakly upregulated in the ΔPdpacC mutant during infection. Disruption of Pdpg2 also resulted in attenuated virulence of P. digitatum towards citrus fruits. Collectively, we conclude that PdPacC plays an important role in pathogenesis of P. digitatum via regulation of the expression of cell wall degradation enzyme genes, such as Pdpg2 and Pdpnl1.
publishDate 2013
dc.date.none.fl_str_mv 2013
2016
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/127732
url http://hdl.handle.net/10261/127732
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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