Ipriflavone-loaded mesoporous nanospheres with potential applications for periodontal treatment.

The incorporation and effects of hollow mesoporous nanospheres in the systemSiO2–CaO (nanoMBGs) containing ipriflavone (IP), a synthetic isoflavone that prevents osteoporosis, were evaluated. Due to their superior porosity and capability to host drugs, these nanoparticles are designed as a potential...

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Autores: Casarrubios Molina, Laura, Gómez Cerezo, María Natividad, Feito Castellano, María José, Vallet Regí, María Dulce Nombre, Arcos Navarrete, Daniel, Portolés Pérez, María Teresa
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/7650
Acceso en línea:https://hdl.handle.net/20.500.14352/7650
Access Level:acceso abierto
Palabra clave:endocytosis
ipriflavone
mesoporous nanospheres
nanoparticles
oxidative stress
pre-osteoblasts
Materiales
3312 Tecnología de Materiales
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spelling Ipriflavone-loaded mesoporous nanospheres with potential applications for periodontal treatment.Casarrubios Molina, LauraGómez Cerezo, María NatividadFeito Castellano, María JoséVallet Regí, María Dulce NombreArcos Navarrete, DanielPortolés Pérez, María Teresaendocytosisipriflavonemesoporous nanospheresnanoparticlesoxidative stresspre-osteoblastsMateriales3312 Tecnología de MaterialesThe incorporation and effects of hollow mesoporous nanospheres in the systemSiO2–CaO (nanoMBGs) containing ipriflavone (IP), a synthetic isoflavone that prevents osteoporosis, were evaluated. Due to their superior porosity and capability to host drugs, these nanoparticles are designed as a potential alternative to conventional bioactive glasses for the treatment of periodontal defects. To identify the endocytic mechanisms by which these nanospheres are incorporated within the MC3T3-E1 cells, five inhibitors (cytochalasin B, cytochalasin D, chlorpromazine, genistein and wortmannin) were used before the addition of these nanoparticles labeled with fluorescein isothiocyanate (FITC–nanoMBGs). The results indicate that nanoMBGs enter the pre-osteoblasts mainly through clathrin-dependent mechanisms and in a lower proportion by macropinocytosis. The present study evidences the active incorporation of nanoMBG–IPs by MC3T3-E1 osteoprogenitor cells that stimulate their differentiation into mature osteoblast phenotype with increased alkaline phosphatase activity. The final aim of this study is to demonstrate the biocompatibility and osteogenic behavior of IP-loaded bioactive nanoparticles to be used for periodontal augmentation purposes and to shed light on internalization mechanisms that determine the incorporation of these nanoparticles into the cells.MDPIUniversidad Complutense de Madrid20202020-12-2120202020-12-21journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/7650reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/76502026-06-02T12:44:21Z
dc.title.none.fl_str_mv Ipriflavone-loaded mesoporous nanospheres with potential applications for periodontal treatment.
title Ipriflavone-loaded mesoporous nanospheres with potential applications for periodontal treatment.
spellingShingle Ipriflavone-loaded mesoporous nanospheres with potential applications for periodontal treatment.
Casarrubios Molina, Laura
endocytosis
ipriflavone
mesoporous nanospheres
nanoparticles
oxidative stress
pre-osteoblasts
Materiales
3312 Tecnología de Materiales
title_short Ipriflavone-loaded mesoporous nanospheres with potential applications for periodontal treatment.
title_full Ipriflavone-loaded mesoporous nanospheres with potential applications for periodontal treatment.
title_fullStr Ipriflavone-loaded mesoporous nanospheres with potential applications for periodontal treatment.
title_full_unstemmed Ipriflavone-loaded mesoporous nanospheres with potential applications for periodontal treatment.
title_sort Ipriflavone-loaded mesoporous nanospheres with potential applications for periodontal treatment.
dc.creator.none.fl_str_mv Casarrubios Molina, Laura
Gómez Cerezo, María Natividad
Feito Castellano, María José
Vallet Regí, María Dulce Nombre
Arcos Navarrete, Daniel
Portolés Pérez, María Teresa
author Casarrubios Molina, Laura
author_facet Casarrubios Molina, Laura
Gómez Cerezo, María Natividad
Feito Castellano, María José
Vallet Regí, María Dulce Nombre
Arcos Navarrete, Daniel
Portolés Pérez, María Teresa
author_role author
author2 Gómez Cerezo, María Natividad
Feito Castellano, María José
Vallet Regí, María Dulce Nombre
Arcos Navarrete, Daniel
Portolés Pérez, María Teresa
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv endocytosis
ipriflavone
mesoporous nanospheres
nanoparticles
oxidative stress
pre-osteoblasts
Materiales
3312 Tecnología de Materiales
topic endocytosis
ipriflavone
mesoporous nanospheres
nanoparticles
oxidative stress
pre-osteoblasts
Materiales
3312 Tecnología de Materiales
description The incorporation and effects of hollow mesoporous nanospheres in the systemSiO2–CaO (nanoMBGs) containing ipriflavone (IP), a synthetic isoflavone that prevents osteoporosis, were evaluated. Due to their superior porosity and capability to host drugs, these nanoparticles are designed as a potential alternative to conventional bioactive glasses for the treatment of periodontal defects. To identify the endocytic mechanisms by which these nanospheres are incorporated within the MC3T3-E1 cells, five inhibitors (cytochalasin B, cytochalasin D, chlorpromazine, genistein and wortmannin) were used before the addition of these nanoparticles labeled with fluorescein isothiocyanate (FITC–nanoMBGs). The results indicate that nanoMBGs enter the pre-osteoblasts mainly through clathrin-dependent mechanisms and in a lower proportion by macropinocytosis. The present study evidences the active incorporation of nanoMBG–IPs by MC3T3-E1 osteoprogenitor cells that stimulate their differentiation into mature osteoblast phenotype with increased alkaline phosphatase activity. The final aim of this study is to demonstrate the biocompatibility and osteogenic behavior of IP-loaded bioactive nanoparticles to be used for periodontal augmentation purposes and to shed light on internalization mechanisms that determine the incorporation of these nanoparticles into the cells.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-12-21
2020
2020-12-21
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/7650
url https://hdl.handle.net/20.500.14352/7650
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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