Polyphenols and Triterpenes Combination in an In Vitro Model of Cardiac Damage: Protective Effects

Olive products contain high levels of monounsaturated fatty acids as well as other minor components such as triterpenic alcohols and other pentacyclic triterpenes, which together form the main triterpenes of virgin olive oil. Olive fruits and leaves contain significant amounts of hydrophilic and lip...

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Detalles Bibliográficos
Autores: de las Heras, Natalia, Galiana, Adrián, Ballesteros, Sandra, Quintela, José Carlos, Ileana, Bonilauri, Vicente, Lahera, Martín-Fernández, Beatriz
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad a Distancia de Madrid (UDIMA)
Repositorio:udiMundus. Repositorio Institucional de la Universidad a Distancia de Madrid
OAI Identifier:oai:udimundus.udima.es:20.500.12226/1526
Acceso en línea:http://hdl.handle.net/20.500.12226/1526
Access Level:acceso abierto
Palabra clave:polyphenols
triterpenes
cardiac cell viability
oxidative stress
inflammation
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spelling Polyphenols and Triterpenes Combination in an In Vitro Model of Cardiac Damage: Protective Effectsde las Heras, NataliaGaliana, AdriánBallesteros, SandraQuintela, José CarlosIleana, BonilauriVicente, LaheraMartín-Fernández, Beatrizpolyphenolstriterpenescardiac cell viabilityoxidative stressinflammationOlive products contain high levels of monounsaturated fatty acids as well as other minor components such as triterpenic alcohols and other pentacyclic triterpenes, which together form the main triterpenes of virgin olive oil. Olive fruits and leaves contain significant amounts of hydrophilic and lipophilic bioactives including flavones, phenolic acids and phenolic alcohols, amongst others. Several studies have shown the benefits of these substances on the cardiovascular system. Regardless, little is known about the specific combination of bioactive compounds in cardiovascular health. Thus, we aimed to test the combination of a triterpenes (TT70) and a polyphenols (HT60) olive oil bioactive extract in H9c2 cells under stress conditions: LPS and H2O2 stimulation. To evaluate the effectiveness of the combination, we measured cell viability, superoxide production and protein expression of caspase 3, eNOS, peNOS, TNF-α and Il-6. Overall, cells stimulated with LPS or H2O2 and co-incubated with the combination of triterpenes and polyphenols had increased cell survival, lower levels of superoxide anion, lower protein expression of eNOS and higher expression of peNOS, increased protein expression of SOD-1 and lower protein expression of TNF-α and Il-6. The specific combination of HT60+TT70 is of great interest for further study as a possible treatment for cardiovascular damage.2022-23Departamento de Psicología y SaludFacultad de Ciencias de la Salud y de la Educación2023info:eu-repo/semantics/articlehttp://hdl.handle.net/20.500.12226/1526reponame:udiMundus. Repositorio Institucional de la Universidad a Distancia de Madridinstname:Universidad a Distancia de Madrid (UDIMA)Inglésinfo:eu-repo/semantics/openAccessoai:udimundus.udima.es:20.500.12226/15262026-06-02T12:44:31Z
dc.title.none.fl_str_mv Polyphenols and Triterpenes Combination in an In Vitro Model of Cardiac Damage: Protective Effects
title Polyphenols and Triterpenes Combination in an In Vitro Model of Cardiac Damage: Protective Effects
spellingShingle Polyphenols and Triterpenes Combination in an In Vitro Model of Cardiac Damage: Protective Effects
de las Heras, Natalia
polyphenols
triterpenes
cardiac cell viability
oxidative stress
inflammation
title_short Polyphenols and Triterpenes Combination in an In Vitro Model of Cardiac Damage: Protective Effects
title_full Polyphenols and Triterpenes Combination in an In Vitro Model of Cardiac Damage: Protective Effects
title_fullStr Polyphenols and Triterpenes Combination in an In Vitro Model of Cardiac Damage: Protective Effects
title_full_unstemmed Polyphenols and Triterpenes Combination in an In Vitro Model of Cardiac Damage: Protective Effects
title_sort Polyphenols and Triterpenes Combination in an In Vitro Model of Cardiac Damage: Protective Effects
dc.creator.none.fl_str_mv de las Heras, Natalia
Galiana, Adrián
Ballesteros, Sandra
Quintela, José Carlos
Ileana, Bonilauri
Vicente, Lahera
Martín-Fernández, Beatriz
author de las Heras, Natalia
author_facet de las Heras, Natalia
Galiana, Adrián
Ballesteros, Sandra
Quintela, José Carlos
Ileana, Bonilauri
Vicente, Lahera
Martín-Fernández, Beatriz
author_role author
author2 Galiana, Adrián
Ballesteros, Sandra
Quintela, José Carlos
Ileana, Bonilauri
Vicente, Lahera
Martín-Fernández, Beatriz
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv polyphenols
triterpenes
cardiac cell viability
oxidative stress
inflammation
topic polyphenols
triterpenes
cardiac cell viability
oxidative stress
inflammation
description Olive products contain high levels of monounsaturated fatty acids as well as other minor components such as triterpenic alcohols and other pentacyclic triterpenes, which together form the main triterpenes of virgin olive oil. Olive fruits and leaves contain significant amounts of hydrophilic and lipophilic bioactives including flavones, phenolic acids and phenolic alcohols, amongst others. Several studies have shown the benefits of these substances on the cardiovascular system. Regardless, little is known about the specific combination of bioactive compounds in cardiovascular health. Thus, we aimed to test the combination of a triterpenes (TT70) and a polyphenols (HT60) olive oil bioactive extract in H9c2 cells under stress conditions: LPS and H2O2 stimulation. To evaluate the effectiveness of the combination, we measured cell viability, superoxide production and protein expression of caspase 3, eNOS, peNOS, TNF-α and Il-6. Overall, cells stimulated with LPS or H2O2 and co-incubated with the combination of triterpenes and polyphenols had increased cell survival, lower levels of superoxide anion, lower protein expression of eNOS and higher expression of peNOS, increased protein expression of SOD-1 and lower protein expression of TNF-α and Il-6. The specific combination of HT60+TT70 is of great interest for further study as a possible treatment for cardiovascular damage.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12226/1526
url http://hdl.handle.net/20.500.12226/1526
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Departamento de Psicología y Salud
Facultad de Ciencias de la Salud y de la Educación
publisher.none.fl_str_mv Departamento de Psicología y Salud
Facultad de Ciencias de la Salud y de la Educación
dc.source.none.fl_str_mv reponame:udiMundus. Repositorio Institucional de la Universidad a Distancia de Madrid
instname:Universidad a Distancia de Madrid (UDIMA)
instname_str Universidad a Distancia de Madrid (UDIMA)
reponame_str udiMundus. Repositorio Institucional de la Universidad a Distancia de Madrid
collection udiMundus. Repositorio Institucional de la Universidad a Distancia de Madrid
repository.name.fl_str_mv
repository.mail.fl_str_mv
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