Detection of cannabinoid receptors CB1 and CB2 within basal ganglia output neurons in macaques: changes following experimental parkinsonism

Abstract Although type 1 cannabinoid receptors (CB1- Rs) are expressed abundantly throughout the brain, the presence of type 2 cannabinoid receptors (CB2Rs) in neurons is still somewhat controversial. Taking advantage of newly designed CB1R and CB2R mRNA riboprobes, we demonstrate by PCR and in situ...

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Autores: Sierra-Martínez, A.S. (Alejandro Salvador)|||/items/0df2ad7d-cc88-41c0-bb01-e7af2236a89a, Luquin, N. (Natasha)|||/items/d9c4b1c6-1975-4a56-aa89-6577a948d43c, Rico, A.J. (Alberto J.)|||/items/7a177958-067e-4900-943e-0271d63a5021, Gomez-Bautista, V. (V.)|||/items/83cbc8ae-c0bc-41de-9369-cbb23978dd7d, Roda, E. (Elvira)|||/items/7ea91585-d2d9-4d11-aabf-4daab728ca1c, Dopeso-Reyes, I.G. (Iria G.)|||/items/2840f4d4-58c6-4b47-a907-54f685e60355, Vazquez, A. (Alfonso)|||/items/7d5cb87e-5c97-4865-aea8-a4694ef0139f, Martinez-Pinilla, E. (Eva)|||/items/d5984b57-f20c-44bd-907b-365fdfe7b09f, Labandeira-Garcia, J.L. (José L.)|||/items/6ff85c09-f91d-4c80-bb94-2763191534eb, Franco, R. (Rafael)|||/items/11f971cd-f36c-4f89-b4d5-8269d33e93fc, Lanciego-Pérez, J.L. (José Luis)|||/items/d2d4dbfe-4daf-4103-89ec-2ebf41b65cd1
Tipo de documento: artigo
Data de publicação:2014
País:España
Recursos:Universidad de Navarra
Repositório:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglês
OAI Identifier:oai:dadun.unav.edu:10171/38272
Acesso em linha:https://hdl.handle.net/10171/38272
Access Level:Acceso aberto
Palavra-chave:GPCRs
Cannabis
Cannabinoid receptor heteromer
Dyskinesia
Globus pallidus
MPTP
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spelling Detection of cannabinoid receptors CB1 and CB2 within basal ganglia output neurons in macaques: changes following experimental parkinsonismSierra-Martínez, A.S. (Alejandro Salvador)|||/items/0df2ad7d-cc88-41c0-bb01-e7af2236a89aLuquin, N. (Natasha)|||/items/d9c4b1c6-1975-4a56-aa89-6577a948d43cRico, A.J. (Alberto J.)|||/items/7a177958-067e-4900-943e-0271d63a5021Gomez-Bautista, V. (V.)|||/items/83cbc8ae-c0bc-41de-9369-cbb23978dd7dRoda, E. (Elvira)|||/items/7ea91585-d2d9-4d11-aabf-4daab728ca1cDopeso-Reyes, I.G. (Iria G.)|||/items/2840f4d4-58c6-4b47-a907-54f685e60355Vazquez, A. (Alfonso)|||/items/7d5cb87e-5c97-4865-aea8-a4694ef0139fMartinez-Pinilla, E. (Eva)|||/items/d5984b57-f20c-44bd-907b-365fdfe7b09fLabandeira-Garcia, J.L. (José L.)|||/items/6ff85c09-f91d-4c80-bb94-2763191534ebFranco, R. (Rafael)|||/items/11f971cd-f36c-4f89-b4d5-8269d33e93fcLanciego-Pérez, J.L. (José Luis)|||/items/d2d4dbfe-4daf-4103-89ec-2ebf41b65cd1GPCRsCannabisCannabinoid receptor heteromerDyskinesiaGlobus pallidusMPTPAbstract Although type 1 cannabinoid receptors (CB1- Rs) are expressed abundantly throughout the brain, the presence of type 2 cannabinoid receptors (CB2Rs) in neurons is still somewhat controversial. Taking advantage of newly designed CB1R and CB2R mRNA riboprobes, we demonstrate by PCR and in situ hybridization that transcripts for both cannabinoid receptors are present within labeled pallidothalamic-projecting neurons of control and MPTP-treated macaques, whereas the expression is markedly reduced in dyskinetic animals. Moreover, an in situ proximity ligation assay was used to qualitatively assess the presence of CB1Rs and CB2Rs, as well as CB1R–CB2R heteromers within basal ganglia output neurons in all animal groups (control, parkinsonian and dyskinetic macaques). A marked reduction in the number of CB1Rs, CB2Rs and CB1R–CB2R heteromers was found in dyskinetic animals, mimicking the observed reduction in CB1R and CB2R mRNA expression levels. The fact that chronic levodopa treatment disrupted CB1R–CB2R heteromeric complexes should be taken into consideration when designing new drugs acting on cannabinoid receptor heteromers.SpringerDadun. Depósito Académico Digital Universidad de Navarra20152015-05-1120142014-01-0120142014-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/38272reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/382722026-06-21T12:47:57Z
dc.title.none.fl_str_mv Detection of cannabinoid receptors CB1 and CB2 within basal ganglia output neurons in macaques: changes following experimental parkinsonism
title Detection of cannabinoid receptors CB1 and CB2 within basal ganglia output neurons in macaques: changes following experimental parkinsonism
spellingShingle Detection of cannabinoid receptors CB1 and CB2 within basal ganglia output neurons in macaques: changes following experimental parkinsonism
Sierra-Martínez, A.S. (Alejandro Salvador)|||/items/0df2ad7d-cc88-41c0-bb01-e7af2236a89a
GPCRs
Cannabis
Cannabinoid receptor heteromer
Dyskinesia
Globus pallidus
MPTP
title_short Detection of cannabinoid receptors CB1 and CB2 within basal ganglia output neurons in macaques: changes following experimental parkinsonism
title_full Detection of cannabinoid receptors CB1 and CB2 within basal ganglia output neurons in macaques: changes following experimental parkinsonism
title_fullStr Detection of cannabinoid receptors CB1 and CB2 within basal ganglia output neurons in macaques: changes following experimental parkinsonism
title_full_unstemmed Detection of cannabinoid receptors CB1 and CB2 within basal ganglia output neurons in macaques: changes following experimental parkinsonism
title_sort Detection of cannabinoid receptors CB1 and CB2 within basal ganglia output neurons in macaques: changes following experimental parkinsonism
dc.creator.none.fl_str_mv Sierra-Martínez, A.S. (Alejandro Salvador)|||/items/0df2ad7d-cc88-41c0-bb01-e7af2236a89a
Luquin, N. (Natasha)|||/items/d9c4b1c6-1975-4a56-aa89-6577a948d43c
Rico, A.J. (Alberto J.)|||/items/7a177958-067e-4900-943e-0271d63a5021
Gomez-Bautista, V. (V.)|||/items/83cbc8ae-c0bc-41de-9369-cbb23978dd7d
Roda, E. (Elvira)|||/items/7ea91585-d2d9-4d11-aabf-4daab728ca1c
Dopeso-Reyes, I.G. (Iria G.)|||/items/2840f4d4-58c6-4b47-a907-54f685e60355
Vazquez, A. (Alfonso)|||/items/7d5cb87e-5c97-4865-aea8-a4694ef0139f
Martinez-Pinilla, E. (Eva)|||/items/d5984b57-f20c-44bd-907b-365fdfe7b09f
Labandeira-Garcia, J.L. (José L.)|||/items/6ff85c09-f91d-4c80-bb94-2763191534eb
Franco, R. (Rafael)|||/items/11f971cd-f36c-4f89-b4d5-8269d33e93fc
Lanciego-Pérez, J.L. (José Luis)|||/items/d2d4dbfe-4daf-4103-89ec-2ebf41b65cd1
author Sierra-Martínez, A.S. (Alejandro Salvador)|||/items/0df2ad7d-cc88-41c0-bb01-e7af2236a89a
author_facet Sierra-Martínez, A.S. (Alejandro Salvador)|||/items/0df2ad7d-cc88-41c0-bb01-e7af2236a89a
Luquin, N. (Natasha)|||/items/d9c4b1c6-1975-4a56-aa89-6577a948d43c
Rico, A.J. (Alberto J.)|||/items/7a177958-067e-4900-943e-0271d63a5021
Gomez-Bautista, V. (V.)|||/items/83cbc8ae-c0bc-41de-9369-cbb23978dd7d
Roda, E. (Elvira)|||/items/7ea91585-d2d9-4d11-aabf-4daab728ca1c
Dopeso-Reyes, I.G. (Iria G.)|||/items/2840f4d4-58c6-4b47-a907-54f685e60355
Vazquez, A. (Alfonso)|||/items/7d5cb87e-5c97-4865-aea8-a4694ef0139f
Martinez-Pinilla, E. (Eva)|||/items/d5984b57-f20c-44bd-907b-365fdfe7b09f
Labandeira-Garcia, J.L. (José L.)|||/items/6ff85c09-f91d-4c80-bb94-2763191534eb
Franco, R. (Rafael)|||/items/11f971cd-f36c-4f89-b4d5-8269d33e93fc
Lanciego-Pérez, J.L. (José Luis)|||/items/d2d4dbfe-4daf-4103-89ec-2ebf41b65cd1
author_role author
author2 Luquin, N. (Natasha)|||/items/d9c4b1c6-1975-4a56-aa89-6577a948d43c
Rico, A.J. (Alberto J.)|||/items/7a177958-067e-4900-943e-0271d63a5021
Gomez-Bautista, V. (V.)|||/items/83cbc8ae-c0bc-41de-9369-cbb23978dd7d
Roda, E. (Elvira)|||/items/7ea91585-d2d9-4d11-aabf-4daab728ca1c
Dopeso-Reyes, I.G. (Iria G.)|||/items/2840f4d4-58c6-4b47-a907-54f685e60355
Vazquez, A. (Alfonso)|||/items/7d5cb87e-5c97-4865-aea8-a4694ef0139f
Martinez-Pinilla, E. (Eva)|||/items/d5984b57-f20c-44bd-907b-365fdfe7b09f
Labandeira-Garcia, J.L. (José L.)|||/items/6ff85c09-f91d-4c80-bb94-2763191534eb
Franco, R. (Rafael)|||/items/11f971cd-f36c-4f89-b4d5-8269d33e93fc
Lanciego-Pérez, J.L. (José Luis)|||/items/d2d4dbfe-4daf-4103-89ec-2ebf41b65cd1
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv GPCRs
Cannabis
Cannabinoid receptor heteromer
Dyskinesia
Globus pallidus
MPTP
topic GPCRs
Cannabis
Cannabinoid receptor heteromer
Dyskinesia
Globus pallidus
MPTP
description Abstract Although type 1 cannabinoid receptors (CB1- Rs) are expressed abundantly throughout the brain, the presence of type 2 cannabinoid receptors (CB2Rs) in neurons is still somewhat controversial. Taking advantage of newly designed CB1R and CB2R mRNA riboprobes, we demonstrate by PCR and in situ hybridization that transcripts for both cannabinoid receptors are present within labeled pallidothalamic-projecting neurons of control and MPTP-treated macaques, whereas the expression is markedly reduced in dyskinetic animals. Moreover, an in situ proximity ligation assay was used to qualitatively assess the presence of CB1Rs and CB2Rs, as well as CB1R–CB2R heteromers within basal ganglia output neurons in all animal groups (control, parkinsonian and dyskinetic macaques). A marked reduction in the number of CB1Rs, CB2Rs and CB1R–CB2R heteromers was found in dyskinetic animals, mimicking the observed reduction in CB1R and CB2R mRNA expression levels. The fact that chronic levodopa treatment disrupted CB1R–CB2R heteromeric complexes should be taken into consideration when designing new drugs acting on cannabinoid receptor heteromers.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01
2014
2014-01-01
2015
2015-05-11
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10171/38272
url https://hdl.handle.net/10171/38272
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra
instname:Universidad de Navarra
instname_str Universidad de Navarra
reponame_str Dadun. Depósito Académico Digital de la Universidad de Navarra
collection Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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