c-Raf regulates cell survival and retinal ganglion cell morphogenesis during neurogenesis

9 pages, 6 figures.

Detalles Bibliográficos
Autores: Pimentel, Belén, Sanz, Carmen, Varela-Nieto, Isabel, Rapp, Ulf R., Pablo, Flora de, De la Rosa, Enrique J.
Tipo de recurso: artículo
Fecha de publicación:2000
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/24261
Acceso en línea:http://hdl.handle.net/10261/24261
Access Level:acceso abierto
Palabra clave:Apoptosis
Cell death
Cell survival
Neural development
Neurogenesis
Retinal ganglion cell
Signaling
Chick embryos
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spelling c-Raf regulates cell survival and retinal ganglion cell morphogenesis during neurogenesisPimentel, BelénSanz, CarmenVarela-Nieto, IsabelRapp, Ulf R.Pablo, Flora deDe la Rosa, Enrique J.ApoptosisCell deathCell survivalNeural developmentNeurogenesisRetinal ganglion cellSignalingChick embryos9 pages, 6 figures.The signaling cascade Ras/Raf/mitogen-activated protein kinases modulates cell proliferation, differentiation, and survival, all key cellular processes during neural development. To better define the in vivo role of Raf during chick retinal neurogenesis, we interfered with Raf-dependent signaling during days 4.5 to 7.5 of embryonic development by expressing a dominant negative mutant of c-Raf (DeltaRaf), which blocks Ras-dependent Raf activation, and by overexpressing wild-type c-Raf. DeltaRaf expression induced an increase in cell death by apoptosis, whereas it did not affect overall cell proliferation and differentiation. In parallel, the number of Islet-1/2-positive and TUJ1-positive retinal ganglion cells were diminished in their definitive layer, whereas there was an increase in the number of mislocated Islet-1/2-positive cells. This disturbed morphogenesis correlated with a disruption of the optic fiber layer. Conversely, c-Raf overexpression caused moderate opposite effects on apoptosis. These results frame in vivo early neurogenesis processes in which c-Raf is essential.This study was supported by Dirección General de Investigación y Desarrollo (Spain) Grants P.M.96–0003 (to E.J.d.l.R.), PB97–0143 (to F.d.P.), and P.M.96–0075 to (I.V.-N.). The fellowships to B.P. and C.S. were awarded by the Ministerio de Educación y Cultura (Spain).Peer reviewedSociety for Neuroscience2000info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/24261reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://www.jneurosci.org/cgi/content/full/20/9/3254info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/242612026-05-22T06:33:51Z
dc.title.none.fl_str_mv c-Raf regulates cell survival and retinal ganglion cell morphogenesis during neurogenesis
title c-Raf regulates cell survival and retinal ganglion cell morphogenesis during neurogenesis
spellingShingle c-Raf regulates cell survival and retinal ganglion cell morphogenesis during neurogenesis
Pimentel, Belén
Apoptosis
Cell death
Cell survival
Neural development
Neurogenesis
Retinal ganglion cell
Signaling
Chick embryos
title_short c-Raf regulates cell survival and retinal ganglion cell morphogenesis during neurogenesis
title_full c-Raf regulates cell survival and retinal ganglion cell morphogenesis during neurogenesis
title_fullStr c-Raf regulates cell survival and retinal ganglion cell morphogenesis during neurogenesis
title_full_unstemmed c-Raf regulates cell survival and retinal ganglion cell morphogenesis during neurogenesis
title_sort c-Raf regulates cell survival and retinal ganglion cell morphogenesis during neurogenesis
dc.creator.none.fl_str_mv Pimentel, Belén
Sanz, Carmen
Varela-Nieto, Isabel
Rapp, Ulf R.
Pablo, Flora de
De la Rosa, Enrique J.
author Pimentel, Belén
author_facet Pimentel, Belén
Sanz, Carmen
Varela-Nieto, Isabel
Rapp, Ulf R.
Pablo, Flora de
De la Rosa, Enrique J.
author_role author
author2 Sanz, Carmen
Varela-Nieto, Isabel
Rapp, Ulf R.
Pablo, Flora de
De la Rosa, Enrique J.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Apoptosis
Cell death
Cell survival
Neural development
Neurogenesis
Retinal ganglion cell
Signaling
Chick embryos
topic Apoptosis
Cell death
Cell survival
Neural development
Neurogenesis
Retinal ganglion cell
Signaling
Chick embryos
description 9 pages, 6 figures.
publishDate 2000
dc.date.none.fl_str_mv 2000
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/24261
url http://hdl.handle.net/10261/24261
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://www.jneurosci.org/cgi/content/full/20/9/3254
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Society for Neuroscience
publisher.none.fl_str_mv Society for Neuroscience
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869409191454572544
score 15.812429

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