Pharmacological evaluation of the anxiolytic-like effects of an aqueous extract of the Raphanus sativus L. sprouts in mice

Raphanus sativus L. (Brassicaceae), commonly known as radish, is consumed worldwide as a vegetable. However, its benefits on mental health are unknown. The aim of this study was to evaluate its anxiolytic-like effects and safety using different experimental models. An aqueous extract of R. sativus s...

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Autores: Hernández-Sánchez, Laura Yunuen, González-Trujano, María Eva, Moreno, Diego A., Vibrans, Heike, Castillo-Juárez, Israel, Dorazco-González, Alejandro, Soto-Hernández, Marcos
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/339431
Acceso en línea:http://hdl.handle.net/10261/339431
Access Level:acceso abierto
Palabra clave:Anxiety
Brassicaceae
Glucosinolates
Mexican traditional medicine
Sulforaphane
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spelling Pharmacological evaluation of the anxiolytic-like effects of an aqueous extract of the Raphanus sativus L. sprouts in miceHernández-Sánchez, Laura YunuenGonzález-Trujano, María EvaMoreno, Diego A.Vibrans, HeikeCastillo-Juárez, IsraelDorazco-González, AlejandroSoto-Hernández, MarcosAnxietyBrassicaceaeGlucosinolatesMexican traditional medicineSulforaphaneRaphanus sativus L. (Brassicaceae), commonly known as radish, is consumed worldwide as a vegetable. However, its benefits on mental health are unknown. The aim of this study was to evaluate its anxiolytic-like effects and safety using different experimental models. An aqueous extract of R. sativus sprouts (AERSS) was pharmacologically evaluated by intraperitoneal route (i.p.) at 10, 30, and 100 mg/kg and orally (p.o.) at 500 mg/kg on behavior by using open-field and plus-maze tests. In addition, its acute toxicity (LD50) was determined by the Lorke's method. Diazepam (1 mg/kg, i.p.) and buspirone (4 mg/kg, i.p.) were the reference drugs. A significant and anxiolytic-like dosage of AERSS (30 mg/kg, i.p.) resembling the effects of reference drugs was chosen to explore the involvement of GABAA/BDZs site (flumazenil, 5 mg/kg, i.p.) and serotonin 5-HT1A receptors (WAY100635, 1 mg/kg, i.p.) as a possible mechanism of action. A 500 mg/kg, p.o. dosage of AERSS produced an anxiolytic-like response equivalent to 100 mg/kg, i.p. No acute toxicity was observed since a LD50 > 2000 mg/kg, i.p. The phytochemical analysis allowed the identification and quantification of major presence of sulforaphene (2500 µM), sulforaphane (15 µM), iberin (0.75 µM), and indol-3-carbinol (0.75 µM), as major constituents. Both the GABAA/BDZs site and serotonin 5-HT1A receptors were involved in the anxiolytic-like activity of AERSS, depending on the pharmacological parameter or the experimental assay tested. Our results demonstrate that the anxiolytic activity of R. sativus sprouts involves GABAA/BDZs site and serotonin 5-HT1A receptors supporting its health benefits in the treatment of anxiety beyond the satisfaction of basic nutritional needsThis work was partially supported by CONACyT (Grant number 256448/226454), INPRFM- NC123280.0 (M.E.G.-T.), and the Fundación Séneca – Murcia Regional Agency for Science and Technology (Comunidad Autónoma de la Región de Murcia, CARM, grant number 20855/PI/18, D.A.M.)Peer reviewedElsevierConsejo Nacional de Humanidades, Ciencias y Tecnologías (México)Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz (México)Fundación SénecaMoreno, Diego A. [0000-0002-6547-8764]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/339431reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésThe underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.1016/j.biopha.2023.114579https://doi.org/10.1016/j.biopha.2023.114579Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3394312026-05-22T06:33:51Z
dc.title.none.fl_str_mv Pharmacological evaluation of the anxiolytic-like effects of an aqueous extract of the Raphanus sativus L. sprouts in mice
title Pharmacological evaluation of the anxiolytic-like effects of an aqueous extract of the Raphanus sativus L. sprouts in mice
spellingShingle Pharmacological evaluation of the anxiolytic-like effects of an aqueous extract of the Raphanus sativus L. sprouts in mice
Hernández-Sánchez, Laura Yunuen
Anxiety
Brassicaceae
Glucosinolates
Mexican traditional medicine
Sulforaphane
title_short Pharmacological evaluation of the anxiolytic-like effects of an aqueous extract of the Raphanus sativus L. sprouts in mice
title_full Pharmacological evaluation of the anxiolytic-like effects of an aqueous extract of the Raphanus sativus L. sprouts in mice
title_fullStr Pharmacological evaluation of the anxiolytic-like effects of an aqueous extract of the Raphanus sativus L. sprouts in mice
title_full_unstemmed Pharmacological evaluation of the anxiolytic-like effects of an aqueous extract of the Raphanus sativus L. sprouts in mice
title_sort Pharmacological evaluation of the anxiolytic-like effects of an aqueous extract of the Raphanus sativus L. sprouts in mice
dc.creator.none.fl_str_mv Hernández-Sánchez, Laura Yunuen
González-Trujano, María Eva
Moreno, Diego A.
Vibrans, Heike
Castillo-Juárez, Israel
Dorazco-González, Alejandro
Soto-Hernández, Marcos
author Hernández-Sánchez, Laura Yunuen
author_facet Hernández-Sánchez, Laura Yunuen
González-Trujano, María Eva
Moreno, Diego A.
Vibrans, Heike
Castillo-Juárez, Israel
Dorazco-González, Alejandro
Soto-Hernández, Marcos
author_role author
author2 González-Trujano, María Eva
Moreno, Diego A.
Vibrans, Heike
Castillo-Juárez, Israel
Dorazco-González, Alejandro
Soto-Hernández, Marcos
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Nacional de Humanidades, Ciencias y Tecnologías (México)
Instituto Nacional de Psiquiatría Ramón de la Fuente Muñiz (México)
Fundación Séneca
Moreno, Diego A. [0000-0002-6547-8764]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Anxiety
Brassicaceae
Glucosinolates
Mexican traditional medicine
Sulforaphane
topic Anxiety
Brassicaceae
Glucosinolates
Mexican traditional medicine
Sulforaphane
description Raphanus sativus L. (Brassicaceae), commonly known as radish, is consumed worldwide as a vegetable. However, its benefits on mental health are unknown. The aim of this study was to evaluate its anxiolytic-like effects and safety using different experimental models. An aqueous extract of R. sativus sprouts (AERSS) was pharmacologically evaluated by intraperitoneal route (i.p.) at 10, 30, and 100 mg/kg and orally (p.o.) at 500 mg/kg on behavior by using open-field and plus-maze tests. In addition, its acute toxicity (LD50) was determined by the Lorke's method. Diazepam (1 mg/kg, i.p.) and buspirone (4 mg/kg, i.p.) were the reference drugs. A significant and anxiolytic-like dosage of AERSS (30 mg/kg, i.p.) resembling the effects of reference drugs was chosen to explore the involvement of GABAA/BDZs site (flumazenil, 5 mg/kg, i.p.) and serotonin 5-HT1A receptors (WAY100635, 1 mg/kg, i.p.) as a possible mechanism of action. A 500 mg/kg, p.o. dosage of AERSS produced an anxiolytic-like response equivalent to 100 mg/kg, i.p. No acute toxicity was observed since a LD50 > 2000 mg/kg, i.p. The phytochemical analysis allowed the identification and quantification of major presence of sulforaphene (2500 µM), sulforaphane (15 µM), iberin (0.75 µM), and indol-3-carbinol (0.75 µM), as major constituents. Both the GABAA/BDZs site and serotonin 5-HT1A receptors were involved in the anxiolytic-like activity of AERSS, depending on the pharmacological parameter or the experimental assay tested. Our results demonstrate that the anxiolytic activity of R. sativus sprouts involves GABAA/BDZs site and serotonin 5-HT1A receptors supporting its health benefits in the treatment of anxiety beyond the satisfaction of basic nutritional needs
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/339431
url http://hdl.handle.net/10261/339431
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv The underlying dataset has been published as supplementary material of the article in the publisher platform at https://doi.org/10.1016/j.biopha.2023.114579
https://doi.org/10.1016/j.biopha.2023.114579
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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