Cell-Driven Encapsulation of Chlorophyllin-Based Carbon Dots within Exosomes for Enhanced Photodynamic Therapy: miRNA Profiling Reveals Mechanistic Insights

Exosomes, nanoscale extracellular vesicles, have emerged as promising carriers in drug delivery due to their ability to bypass biological barriers, low toxicity, high stability, and intrinsic targeting capabilities. Mesenchymal stem-cell-derived exosomes (EXOMSC), with their natural tropism toward t...

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Autores: Besbinar, Omur, Uyar, Recep, Kirbas Cilingir, Emel, Martín-Pardillos, Ana, Hueso, Jose L., Ceylan, Ahmet, Ozgenc, Ozge, Ekim, Okan, Unal, Mehmet Altay, Ari, Fikret, Leblanc, Roger M., Santamaria, Jesus, Yilmazer, Acelya
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Universidad de Zaragoza
Repositorio:Zaguán. Repositorio Digital de la Universidad de Zaragoza
OAI Identifier:oai:zaguan.unizar.es:165154
Acceso en línea:http://zaguan.unizar.es/record/165154
Access Level:acceso abierto
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spelling Cell-Driven Encapsulation of Chlorophyllin-Based Carbon Dots within Exosomes for Enhanced Photodynamic Therapy: miRNA Profiling Reveals Mechanistic InsightsBesbinar, OmurUyar, RecepKirbas Cilingir, EmelMartín-Pardillos, AnaHueso, Jose L.Ceylan, AhmetOzgenc, OzgeEkim, OkanUnal, Mehmet AltayAri, FikretLeblanc, Roger M.Santamaria, JesusYilmazer, AcelyaExosomes, nanoscale extracellular vesicles, have emerged as promising carriers in drug delivery due to their ability to bypass biological barriers, low toxicity, high stability, and intrinsic targeting capabilities. Mesenchymal stem-cell-derived exosomes (EXOMSC), with their natural tropism toward the tumor microenvironment, offer an ideal platform for enhancing therapeutic cargo delivery. In this study, we demonstrate an approach where red-emission chlorophyll-based carbon dots (Chl-CDs) were encapsulated within EXOMSC through a cell-driven uptake mechanism, creating CD@EXOMSC. These exosomes achieved superior photodynamic therapy (PDT) efficacy, requiring 40 times less nanomaterial compared to freestanding Chl-CDs. Mechanistic insights from glioblastoma cell miRNA profiling revealed that the enhanced efficacy was mediated by the regulation of efflux transporter genes, oxidative stress responses, and endocytosis pathways. This work highlights the synergistic potential of combining photosensitizers and miRNA-rich exosomes to achieve targeted and sustained therapeutic delivery, paving the way for a multifaceted approach in cancer therapy.2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://zaguan.unizar.es/record/165154reponame:Zaguán. Repositorio Digital de la Universidad de Zaragozainstname:Universidad de ZaragozaInglésinfo:eu-repo/grantAgreement/ES/AEI/PID2023-148732NB-I00info:eu-repo/grantAgreement/ES/UZ-DGA/T57-23Rinfo:eu-repo/grantAgreement/ES/MICINN/CNS2022-135911info:eu-repo/grantAgreement/ES/MICIU/CEX2023-001286-Sinfo:eu-repo/semantics/openAccessoai:zaguan.unizar.es:1651542026-05-29T13:59:51Z
dc.title.none.fl_str_mv Cell-Driven Encapsulation of Chlorophyllin-Based Carbon Dots within Exosomes for Enhanced Photodynamic Therapy: miRNA Profiling Reveals Mechanistic Insights
title Cell-Driven Encapsulation of Chlorophyllin-Based Carbon Dots within Exosomes for Enhanced Photodynamic Therapy: miRNA Profiling Reveals Mechanistic Insights
spellingShingle Cell-Driven Encapsulation of Chlorophyllin-Based Carbon Dots within Exosomes for Enhanced Photodynamic Therapy: miRNA Profiling Reveals Mechanistic Insights
Besbinar, Omur
title_short Cell-Driven Encapsulation of Chlorophyllin-Based Carbon Dots within Exosomes for Enhanced Photodynamic Therapy: miRNA Profiling Reveals Mechanistic Insights
title_full Cell-Driven Encapsulation of Chlorophyllin-Based Carbon Dots within Exosomes for Enhanced Photodynamic Therapy: miRNA Profiling Reveals Mechanistic Insights
title_fullStr Cell-Driven Encapsulation of Chlorophyllin-Based Carbon Dots within Exosomes for Enhanced Photodynamic Therapy: miRNA Profiling Reveals Mechanistic Insights
title_full_unstemmed Cell-Driven Encapsulation of Chlorophyllin-Based Carbon Dots within Exosomes for Enhanced Photodynamic Therapy: miRNA Profiling Reveals Mechanistic Insights
title_sort Cell-Driven Encapsulation of Chlorophyllin-Based Carbon Dots within Exosomes for Enhanced Photodynamic Therapy: miRNA Profiling Reveals Mechanistic Insights
dc.creator.none.fl_str_mv Besbinar, Omur
Uyar, Recep
Kirbas Cilingir, Emel
Martín-Pardillos, Ana
Hueso, Jose L.
Ceylan, Ahmet
Ozgenc, Ozge
Ekim, Okan
Unal, Mehmet Altay
Ari, Fikret
Leblanc, Roger M.
Santamaria, Jesus
Yilmazer, Acelya
author Besbinar, Omur
author_facet Besbinar, Omur
Uyar, Recep
Kirbas Cilingir, Emel
Martín-Pardillos, Ana
Hueso, Jose L.
Ceylan, Ahmet
Ozgenc, Ozge
Ekim, Okan
Unal, Mehmet Altay
Ari, Fikret
Leblanc, Roger M.
Santamaria, Jesus
Yilmazer, Acelya
author_role author
author2 Uyar, Recep
Kirbas Cilingir, Emel
Martín-Pardillos, Ana
Hueso, Jose L.
Ceylan, Ahmet
Ozgenc, Ozge
Ekim, Okan
Unal, Mehmet Altay
Ari, Fikret
Leblanc, Roger M.
Santamaria, Jesus
Yilmazer, Acelya
author2_role author
author
author
author
author
author
author
author
author
author
author
author
description Exosomes, nanoscale extracellular vesicles, have emerged as promising carriers in drug delivery due to their ability to bypass biological barriers, low toxicity, high stability, and intrinsic targeting capabilities. Mesenchymal stem-cell-derived exosomes (EXOMSC), with their natural tropism toward the tumor microenvironment, offer an ideal platform for enhancing therapeutic cargo delivery. In this study, we demonstrate an approach where red-emission chlorophyll-based carbon dots (Chl-CDs) were encapsulated within EXOMSC through a cell-driven uptake mechanism, creating CD@EXOMSC. These exosomes achieved superior photodynamic therapy (PDT) efficacy, requiring 40 times less nanomaterial compared to freestanding Chl-CDs. Mechanistic insights from glioblastoma cell miRNA profiling revealed that the enhanced efficacy was mediated by the regulation of efflux transporter genes, oxidative stress responses, and endocytosis pathways. This work highlights the synergistic potential of combining photosensitizers and miRNA-rich exosomes to achieve targeted and sustained therapeutic delivery, paving the way for a multifaceted approach in cancer therapy.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://zaguan.unizar.es/record/165154
url http://zaguan.unizar.es/record/165154
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/ES/AEI/PID2023-148732NB-I00
info:eu-repo/grantAgreement/ES/UZ-DGA/T57-23R
info:eu-repo/grantAgreement/ES/MICINN/CNS2022-135911
info:eu-repo/grantAgreement/ES/MICIU/CEX2023-001286-S
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv
publisher.none.fl_str_mv
dc.source.none.fl_str_mv reponame:Zaguán. Repositorio Digital de la Universidad de Zaragoza
instname:Universidad de Zaragoza
instname_str Universidad de Zaragoza
reponame_str Zaguán. Repositorio Digital de la Universidad de Zaragoza
collection Zaguán. Repositorio Digital de la Universidad de Zaragoza
repository.name.fl_str_mv
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