PYL8 ABA receptors of Phoenix dactylifera play a crucial role in response to abiotic stress and are stabilized by ABA.

[EN] The identification of those prevalent abscisic acid (ABA) receptors and molecular mechanisms that trigger drought adaptation in crops well adapted to harsh conditions such as date palm (Phoenix dactylifera, Pd) sheds light on plant-environment interactions. We reveal that PdPYL8-like receptors...

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Detalles Bibliográficos
Autores: García-Maquilón, Irene, Coego, Alberto, Messerer, Maxim, de Ollas, Carlos, Julian, Jose, Pizzio, Gaston, Gómez-Cadenas, Aurelio, Mayer, Klaus F. X., Geiger, Dietmar, Alquraishi, Saleh A., Alrefaei, Abdulwahed F., Ache, Peter, Hedrich, Rainer, Lozano-Juste, Jorge|||0000-0001-7034-566X, Ruiz-Partida, Rafael, Belda Palazón, Borja, Rodríguez Egea, Pedro Luís|||0000-0002-5886-9425
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/190532
Acceso en línea:https://riunet.upv.es/handle/10251/190532
Access Level:acceso abierto
Palabra clave:Phoenix dactylifera
ABA receptor
AHG1
Abscisic acid
PP2C
PYL8
Date palm
Descripción
Sumario:[EN] The identification of those prevalent abscisic acid (ABA) receptors and molecular mechanisms that trigger drought adaptation in crops well adapted to harsh conditions such as date palm (Phoenix dactylifera, Pd) sheds light on plant-environment interactions. We reveal that PdPYL8-like receptors are predominantly expressed under abiotic stress, with Pd27 being the most expressed receptor in date palm. Therefore, subfamily I PdPYL8-like receptors have been selected for ABA signaling during abiotic stress response in this crop. Biochemical characterization of PdPYL8-like and PdPYL1-like receptors revealed receptor- and ABA-dependent inhibition of PP2Cs, which triggers activation of the pRD29B-LUC reporter in response to ABA. PdPYLs efficiently abolish PP2C-mediated repression of ABA signaling, but loss of the Trp lock in the seed-specific AHG1-like phosphatase PdPP2C79 markedly impairs its inhibition by ABA receptors. Characterization of Arabidopsis transgenic plants that express PdPYLs shows enhanced ABA signaling in seed, root, and guard cells. Specifically, Pd27-overexpressing plants showed lower ABA content and were more efficient than the wild type in lowering transpiration at negative soil water potential, leading to enhanced drought tolerance. Finally, PdPYL8-like receptors accumulate after ABA treatment, which suggests that ABA-induced stabilization of these receptors operates in date palm for efficient boosting of ABA signaling in response to abiotic stress.

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