Trap-TRAP, a versatile tool for tissue-specific translatomics in zebrafish

Developmental and physiological processes depend on the transcriptional and translational activity of heterogeneous cell populations. A main challenge in gene expression studies is dealing with this intrinsic complexity while keeping sequencing efficiency. Translating ribosome affinity purification...

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Detalles Bibliográficos
Autores: Corbacho Sánchez, Jorge, Sanabria-Reinoso, Estefanía, Buono, Lorena, Fernández-Miñán, Ana, Martínez-Morales, Juan Ramón
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/281501
Acceso en línea:http://hdl.handle.net/10261/281501
Access Level:acceso abierto
Palabra clave:Enhancer-trap
Gal4/UAS
Zebrafish
TRAP
Translatome
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spelling Trap-TRAP, a versatile tool for tissue-specific translatomics in zebrafishCorbacho Sánchez, JorgeSanabria-Reinoso, EstefaníaBuono, LorenaFernández-Miñán, AnaMartínez-Morales, Juan RamónEnhancer-trapGal4/UASZebrafishTRAPTranslatomeDevelopmental and physiological processes depend on the transcriptional and translational activity of heterogeneous cell populations. A main challenge in gene expression studies is dealing with this intrinsic complexity while keeping sequencing efficiency. Translating ribosome affinity purification (TRAP) methods have allowed cell-specific recovery of polyribosome-associated RNAs by genetic tagging of ribosomes in selected cell populations. Here we combined the TRAP approach with adapted enhancer trap methods (trap-TRAP) to systematically generate zebrafish transgenic lines suitable for tissue-specific translatome interrogation. Through the random integration of a GFP-tagged version of the large subunit ribosomal protein L10a (EGFP-Rpl10a), we have generated stable lines driving expression in a variety of tissues, including the retina, skeletal muscle, lateral line primordia, rhombomeres, or jaws. To increase the range of applications, a UAS:TRAP transgenic line compatible with available Gal4 lines was also generated and tested. The resulting collection of lines and applications constitutes a resource for the zebrafish community in developmental genetics, organ physiology and disease modelling.This work is supported by grants awarded to JM-M from the Fundacio´n Ramón Areces (program-2016); PY20_00006 from Junta de Andalucía; as well as Spanish Ministry of Science, Innovation and Universities (MICINN, AEI/FEDER) BFU2017-91324-EXP, BFU2017-86339P, RED2018-102553-T, PID2020-112566GB-I00, and MDM-2016-0687.Frontiers MediaFundación Ramón ArecesJunta de AndalucíaMinisterio de Economía y Competitividad (España)Ministerio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/281501reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement///info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2017-86339-Pinfo:eu-repo/grantAgreement///info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-112566GB-I00info:eu-repo/grantAgreement/MINECO//MDM-2016-0687http://dx.doi.org/10.3389/fcell.2021.817191Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2815012026-05-22T06:33:51Z
dc.title.none.fl_str_mv Trap-TRAP, a versatile tool for tissue-specific translatomics in zebrafish
title Trap-TRAP, a versatile tool for tissue-specific translatomics in zebrafish
spellingShingle Trap-TRAP, a versatile tool for tissue-specific translatomics in zebrafish
Corbacho Sánchez, Jorge
Enhancer-trap
Gal4/UAS
Zebrafish
TRAP
Translatome
title_short Trap-TRAP, a versatile tool for tissue-specific translatomics in zebrafish
title_full Trap-TRAP, a versatile tool for tissue-specific translatomics in zebrafish
title_fullStr Trap-TRAP, a versatile tool for tissue-specific translatomics in zebrafish
title_full_unstemmed Trap-TRAP, a versatile tool for tissue-specific translatomics in zebrafish
title_sort Trap-TRAP, a versatile tool for tissue-specific translatomics in zebrafish
dc.creator.none.fl_str_mv Corbacho Sánchez, Jorge
Sanabria-Reinoso, Estefanía
Buono, Lorena
Fernández-Miñán, Ana
Martínez-Morales, Juan Ramón
author Corbacho Sánchez, Jorge
author_facet Corbacho Sánchez, Jorge
Sanabria-Reinoso, Estefanía
Buono, Lorena
Fernández-Miñán, Ana
Martínez-Morales, Juan Ramón
author_role author
author2 Sanabria-Reinoso, Estefanía
Buono, Lorena
Fernández-Miñán, Ana
Martínez-Morales, Juan Ramón
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Fundación Ramón Areces
Junta de Andalucía
Ministerio de Economía y Competitividad (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Enhancer-trap
Gal4/UAS
Zebrafish
TRAP
Translatome
topic Enhancer-trap
Gal4/UAS
Zebrafish
TRAP
Translatome
description Developmental and physiological processes depend on the transcriptional and translational activity of heterogeneous cell populations. A main challenge in gene expression studies is dealing with this intrinsic complexity while keeping sequencing efficiency. Translating ribosome affinity purification (TRAP) methods have allowed cell-specific recovery of polyribosome-associated RNAs by genetic tagging of ribosomes in selected cell populations. Here we combined the TRAP approach with adapted enhancer trap methods (trap-TRAP) to systematically generate zebrafish transgenic lines suitable for tissue-specific translatome interrogation. Through the random integration of a GFP-tagged version of the large subunit ribosomal protein L10a (EGFP-Rpl10a), we have generated stable lines driving expression in a variety of tissues, including the retina, skeletal muscle, lateral line primordia, rhombomeres, or jaws. To increase the range of applications, a UAS:TRAP transgenic line compatible with available Gal4 lines was also generated and tested. The resulting collection of lines and applications constitutes a resource for the zebrafish community in developmental genetics, organ physiology and disease modelling.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/281501
url http://hdl.handle.net/10261/281501
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement///
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2017-86339-P
info:eu-repo/grantAgreement///
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-112566GB-I00
info:eu-repo/grantAgreement/MINECO//MDM-2016-0687
http://dx.doi.org/10.3389/fcell.2021.817191
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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