Comparative gene expression of gonadotropins (FSH and LH) and peptide levels of gonadotropin-releasing hormones (GnRHs) in the pituitary of wild and cultured Senegalese sole (Solea senegalensis) broodstocks

12 páginas, 8 figuras, 2 tablas.

Detalhes bibliográficos
Autores: Guzmán, José M., Rubio, M., Ortiz-Delgado, Juan B., Klenke, U., Kight, Katherine, Cross, Ismael, Sánchez-Ramos, I., Riaza, Ana, Rebordinos, Laureana, Sarasquete, Carmen, Mañanós, Evaristo L.
Formato: artículo
Fecha de publicación:2009
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/43064
Acesso em linha:http://hdl.handle.net/10261/43064
Access Level:acceso abierto
Palavra-chave:FSH
Gonadotropin
GnRH
LH
Reproductive dysfunction
Senegalese sole
Solea senegalensis
Spawning season
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spelling Comparative gene expression of gonadotropins (FSH and LH) and peptide levels of gonadotropin-releasing hormones (GnRHs) in the pituitary of wild and cultured Senegalese sole (Solea senegalensis) broodstocksGuzmán, José M.Rubio, M.Ortiz-Delgado, Juan B.Klenke, U.Kight, KatherineCross, IsmaelSánchez-Ramos, I.Riaza, AnaRebordinos, LaureanaSarasquete, CarmenMañanós, Evaristo L.FSHGonadotropinGnRHLHReproductive dysfunctionSenegalese soleSolea senegalensisSpawning season12 páginas, 8 figuras, 2 tablas.The Senegalese sole (Solea senegalensis) is a valuable flatfish for aquaculture, but it presents important reproductive problems in captivity. Spawning is achieved by wild-caught breeders but cultured broodstocks fail to spawn spontaneously and, when they do, eggs are unfertilized. To gain knowledge on the physiological basis underlying this reproductive dysfunction, this study aimed at analyzing comparative hormone levels between wild and cultured broodstocks at the spawning season. The Senegalese sole gonadotropin (GTH) subunits, FSHβ, LHβ and GPα, were cloned and qualitative (in situ hybridization) and quantitative (real-time PCR) assays developed to analyze pituitary GTH gene expression. In females, FSHβ and GPα mRNA levels were higher in wild than in cultured broodstocks, whereas in males all three subunits were highest in cultured. By ELISA, three GnRH forms were detected in the pituitary, displaying a relative abundance of GnRH2 > GnRH1 > GnRH3. All GnRHs were slightly more abundant in wild than cultured females, whereas no differences were observed in males. Plasma levels of vitellogenin and sex steroids were also analyzed. Results showed endocrine differences between wild and cultured broodstocks at the spawning period, which could be related to the endocrine failure of the reproductive axis in cultured breeders.This research was funded by the Spanish Ministry of Education and Science (MEC) (AGL2006-13777-C01), the Ministry of Agriculture, Fisheries and Food (MAPA) (JACUMAR, II National Plan for the Cultivation of Sole) and the Regional Government of Galicia (PGIDIT06RMA004E). J.M. Guzmán received a FPI fellowship from the MEC. J.B. Ortiz-Delgado was supported by the “Ramón y Cajal” program (MEC, Spain).Peer reviewedElsevier201120112009info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/43064reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.cbpa.2009.02.032info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/430642026-05-22T06:33:51Z
dc.title.none.fl_str_mv Comparative gene expression of gonadotropins (FSH and LH) and peptide levels of gonadotropin-releasing hormones (GnRHs) in the pituitary of wild and cultured Senegalese sole (Solea senegalensis) broodstocks
title Comparative gene expression of gonadotropins (FSH and LH) and peptide levels of gonadotropin-releasing hormones (GnRHs) in the pituitary of wild and cultured Senegalese sole (Solea senegalensis) broodstocks
spellingShingle Comparative gene expression of gonadotropins (FSH and LH) and peptide levels of gonadotropin-releasing hormones (GnRHs) in the pituitary of wild and cultured Senegalese sole (Solea senegalensis) broodstocks
Guzmán, José M.
FSH
Gonadotropin
GnRH
LH
Reproductive dysfunction
Senegalese sole
Solea senegalensis
Spawning season
title_short Comparative gene expression of gonadotropins (FSH and LH) and peptide levels of gonadotropin-releasing hormones (GnRHs) in the pituitary of wild and cultured Senegalese sole (Solea senegalensis) broodstocks
title_full Comparative gene expression of gonadotropins (FSH and LH) and peptide levels of gonadotropin-releasing hormones (GnRHs) in the pituitary of wild and cultured Senegalese sole (Solea senegalensis) broodstocks
title_fullStr Comparative gene expression of gonadotropins (FSH and LH) and peptide levels of gonadotropin-releasing hormones (GnRHs) in the pituitary of wild and cultured Senegalese sole (Solea senegalensis) broodstocks
title_full_unstemmed Comparative gene expression of gonadotropins (FSH and LH) and peptide levels of gonadotropin-releasing hormones (GnRHs) in the pituitary of wild and cultured Senegalese sole (Solea senegalensis) broodstocks
title_sort Comparative gene expression of gonadotropins (FSH and LH) and peptide levels of gonadotropin-releasing hormones (GnRHs) in the pituitary of wild and cultured Senegalese sole (Solea senegalensis) broodstocks
dc.creator.none.fl_str_mv Guzmán, José M.
Rubio, M.
Ortiz-Delgado, Juan B.
Klenke, U.
Kight, Katherine
Cross, Ismael
Sánchez-Ramos, I.
Riaza, Ana
Rebordinos, Laureana
Sarasquete, Carmen
Mañanós, Evaristo L.
author Guzmán, José M.
author_facet Guzmán, José M.
Rubio, M.
Ortiz-Delgado, Juan B.
Klenke, U.
Kight, Katherine
Cross, Ismael
Sánchez-Ramos, I.
Riaza, Ana
Rebordinos, Laureana
Sarasquete, Carmen
Mañanós, Evaristo L.
author_role author
author2 Rubio, M.
Ortiz-Delgado, Juan B.
Klenke, U.
Kight, Katherine
Cross, Ismael
Sánchez-Ramos, I.
Riaza, Ana
Rebordinos, Laureana
Sarasquete, Carmen
Mañanós, Evaristo L.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FSH
Gonadotropin
GnRH
LH
Reproductive dysfunction
Senegalese sole
Solea senegalensis
Spawning season
topic FSH
Gonadotropin
GnRH
LH
Reproductive dysfunction
Senegalese sole
Solea senegalensis
Spawning season
description 12 páginas, 8 figuras, 2 tablas.
publishDate 2009
dc.date.none.fl_str_mv 2009
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/43064
url http://hdl.handle.net/10261/43064
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.cbpa.2009.02.032
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869409082632306688
score 15.81155

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