Antimicrobial Resistance Profiles and Characterization of Escherichia coli Strains from Cases of Neonatal Diarrhea in Spanish Pig Farms

Escherichia coli is considered one of the most common agents associated with neonatal diarrhea in piglets. The aim of this work was to characterize the pathogenic and antimicrobial resistance (AMR) profiles of 122 E. coli strains isolated from pigs suffering diarrhea (n = 94) and pigs without diarrh...

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Autores: Vidal, Anna, Aguirre Molins, Laia|||0000-0003-3257-3965, Seminati, Chiara, Tello, Montse, Redondo, Noelia, Martín Castillo, Margarita|||0000-0003-4370-6700, Darwich Soliva, Laila|||0000-0001-9027-9452
Formato: artículo
Fecha de publicación:2020
País:España
Recursos:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:230728
Acesso em linha:https://ddd.uab.cat/record/230728
https://dx.doi.org/urn:doi:10.3390/vetsci7020048
Access Level:acceso abierto
Palavra-chave:Antimicrobial resistance
ESBL genes
Colistin resistance
Escherichia coli
Neonatal diarrhea
Pig
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spelling Antimicrobial Resistance Profiles and Characterization of Escherichia coli Strains from Cases of Neonatal Diarrhea in Spanish Pig FarmsVidal, AnnaAguirre Molins, Laia|||0000-0003-3257-3965Seminati, ChiaraTello, MontseRedondo, NoeliaMartín Castillo, Margarita|||0000-0003-4370-6700Darwich Soliva, Laila|||0000-0001-9027-9452Antimicrobial resistanceESBL genesColistin resistanceEscherichia coliNeonatal diarrheaPigEscherichia coli is considered one of the most common agents associated with neonatal diarrhea in piglets. The aim of this work was to characterize the pathogenic and antimicrobial resistance (AMR) profiles of 122 E. coli strains isolated from pigs suffering diarrhea (n = 94) and pigs without diarrhea (n = 28) of 24 farms in Spain. Virulence factors, toxins and AMR (ESBL and colistin) genes and AMR phenotypes of E. coli isolates were analyzed. Low prevalence of pathogenic E. coli strains (26%) was found in both groups. However, ETEC and VTEC strains were more frequently isolated from diarrheic piglets. Irrespectively of diarrhea occurrence, 97.5% of the strains showed a multidrug-resistance (MDR) profile to aminopenicillins, sulfonamides and tetracyclines. It was found that 22% of E. coli was CTX-M+, with CTX-M-14 being the principal allelic variant. Remarkably, 81.5% of CTX-M+ strains were isolated from diarrheic animals and presented an extended MDR profile to aminopenicillins, quinolones and aminoglycosides. Finally, low frequencies of colistin resistance genes mcr -1 (4/122) and mcr -4 (1/122) were found. MDR E. coli strains are circulating in pig farms of Spain, representing a serious threat to animal and public health. More appropriate diagnostic approaches (genetic and AMR phenotypic analysis) should be implemented in animal health to optimize antibiotic treatments. 22020-01-0120202020-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/230728https://dx.doi.org/urn:doi:10.3390/vetsci7020048reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2307282026-06-06T12:50:31Z
dc.title.none.fl_str_mv Antimicrobial Resistance Profiles and Characterization of Escherichia coli Strains from Cases of Neonatal Diarrhea in Spanish Pig Farms
title Antimicrobial Resistance Profiles and Characterization of Escherichia coli Strains from Cases of Neonatal Diarrhea in Spanish Pig Farms
spellingShingle Antimicrobial Resistance Profiles and Characterization of Escherichia coli Strains from Cases of Neonatal Diarrhea in Spanish Pig Farms
Vidal, Anna
Antimicrobial resistance
ESBL genes
Colistin resistance
Escherichia coli
Neonatal diarrhea
Pig
title_short Antimicrobial Resistance Profiles and Characterization of Escherichia coli Strains from Cases of Neonatal Diarrhea in Spanish Pig Farms
title_full Antimicrobial Resistance Profiles and Characterization of Escherichia coli Strains from Cases of Neonatal Diarrhea in Spanish Pig Farms
title_fullStr Antimicrobial Resistance Profiles and Characterization of Escherichia coli Strains from Cases of Neonatal Diarrhea in Spanish Pig Farms
title_full_unstemmed Antimicrobial Resistance Profiles and Characterization of Escherichia coli Strains from Cases of Neonatal Diarrhea in Spanish Pig Farms
title_sort Antimicrobial Resistance Profiles and Characterization of Escherichia coli Strains from Cases of Neonatal Diarrhea in Spanish Pig Farms
dc.creator.none.fl_str_mv Vidal, Anna
Aguirre Molins, Laia|||0000-0003-3257-3965
Seminati, Chiara
Tello, Montse
Redondo, Noelia
Martín Castillo, Margarita|||0000-0003-4370-6700
Darwich Soliva, Laila|||0000-0001-9027-9452
author Vidal, Anna
author_facet Vidal, Anna
Aguirre Molins, Laia|||0000-0003-3257-3965
Seminati, Chiara
Tello, Montse
Redondo, Noelia
Martín Castillo, Margarita|||0000-0003-4370-6700
Darwich Soliva, Laila|||0000-0001-9027-9452
author_role author
author2 Aguirre Molins, Laia|||0000-0003-3257-3965
Seminati, Chiara
Tello, Montse
Redondo, Noelia
Martín Castillo, Margarita|||0000-0003-4370-6700
Darwich Soliva, Laila|||0000-0001-9027-9452
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Antimicrobial resistance
ESBL genes
Colistin resistance
Escherichia coli
Neonatal diarrhea
Pig
topic Antimicrobial resistance
ESBL genes
Colistin resistance
Escherichia coli
Neonatal diarrhea
Pig
description Escherichia coli is considered one of the most common agents associated with neonatal diarrhea in piglets. The aim of this work was to characterize the pathogenic and antimicrobial resistance (AMR) profiles of 122 E. coli strains isolated from pigs suffering diarrhea (n = 94) and pigs without diarrhea (n = 28) of 24 farms in Spain. Virulence factors, toxins and AMR (ESBL and colistin) genes and AMR phenotypes of E. coli isolates were analyzed. Low prevalence of pathogenic E. coli strains (26%) was found in both groups. However, ETEC and VTEC strains were more frequently isolated from diarrheic piglets. Irrespectively of diarrhea occurrence, 97.5% of the strains showed a multidrug-resistance (MDR) profile to aminopenicillins, sulfonamides and tetracyclines. It was found that 22% of E. coli was CTX-M+, with CTX-M-14 being the principal allelic variant. Remarkably, 81.5% of CTX-M+ strains were isolated from diarrheic animals and presented an extended MDR profile to aminopenicillins, quinolones and aminoglycosides. Finally, low frequencies of colistin resistance genes mcr -1 (4/122) and mcr -4 (1/122) were found. MDR E. coli strains are circulating in pig farms of Spain, representing a serious threat to animal and public health. More appropriate diagnostic approaches (genetic and AMR phenotypic analysis) should be implemented in animal health to optimize antibiotic treatments.
publishDate 2020
dc.date.none.fl_str_mv 2
2020-01-01
2020
2020-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/230728
https://dx.doi.org/urn:doi:10.3390/vetsci7020048
url https://ddd.uab.cat/record/230728
https://dx.doi.org/urn:doi:10.3390/vetsci7020048
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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