Effect of RONS-Induced Intracellular Redox Homeostasis in 6-NBDG/Glucose Uptake in C2C12 Myotubes and Single Isolated Skeletal Muscle Fibres

[EN] The glucose uptake in skeletal muscle is essential to produce energy through ATP, which is needed by this organ to maintain vital functions. The impairment of glucose uptake compromises the metabolism and function of skeletal muscle and other organs and is a feature of diabetes, obesity, and ag...

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Autores: Martín-Prieto, Eva, Márquez, Carlos Manuel, Fernández-Puente, Escarlata, Palomero Labajos, Jesús
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/154065
Acceso en línea:http://hdl.handle.net/10366/154065
Access Level:acceso abierto
Palabra clave:Glucose uptake
6-NBDG
insulin resistance
C2C12 myotubes
Skeletal muscle fibres
ROS
Hydrogen peroxide
Nitric oxide
Redox homeostasis
Quantitative fluorescence microscopy
Muscle Fibers, Skeletal
Homeostasis
Glucose
Nitric Oxide
Insulin Resistance
Hydrogen Peroxide
3209 Farmacología
2411.10 Fisiología del Músculo
fibras musculares esqueléticas
peróxido de hidrógeno
óxido nítrico
homeostasis
resistencia a la insulina
glucosa
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oai_identifier_str oai:gredos.usal.es:10366/154065
network_acronym_str ES
network_name_str España
repository_id_str
spelling Effect of RONS-Induced Intracellular Redox Homeostasis in 6-NBDG/Glucose Uptake in C2C12 Myotubes and Single Isolated Skeletal Muscle FibresMartín-Prieto, EvaMárquez, Carlos ManuelFernández-Puente, EscarlataPalomero Labajos, JesúsGlucose uptake6-NBDGinsulin resistanceC2C12 myotubesSkeletal muscle fibresROSHydrogen peroxideNitric oxideRedox homeostasisQuantitative fluorescence microscopyMuscle Fibers, SkeletalHomeostasisGlucoseNitric OxideInsulin ResistanceHydrogen Peroxide3209 Farmacología2411.10 Fisiología del Músculofibras musculares esqueléticasperóxido de hidrógenoóxido nítricohomeostasisresistencia a la insulinaglucosa[EN] The glucose uptake in skeletal muscle is essential to produce energy through ATP, which is needed by this organ to maintain vital functions. The impairment of glucose uptake compromises the metabolism and function of skeletal muscle and other organs and is a feature of diabetes, obesity, and ageing. There is a need for research to uncover the mechanisms involved in the impairment of glucose uptake in skeletal muscle. In this study, we adapted, developed, optimised, and validated a methodology based on the fluorescence glucose analogue 6-NBDG, combined with a quantitative fluorescence microscopy image analysis, to determine the glucose uptake in two models of skeletal muscle cells: C2C12 myotubes and single fibres isolated from muscle. It was proposed that reactive oxygen and nitrogen species (RONS) and redox homeostasis play an important role in the modulation of intracellular redox signalling pathways associated with glucose uptake. In this study, we prove that the prooxidative intracellular redox environment under oxidative eustress produced by RONS such as hydrogen peroxide and nitric oxide improves glucose uptake in skeletal muscle cells. However, when oxidation is excessive, oxidative distress occurs, and cellular viability is compromised, although there might be an increase in the glucose uptake. Based on the results of this study, the determination of 6-NBDG/glucose uptake in myotubes and skeletal muscle cells is feasible, validated, and will contribute to improve future research.MDPI202420242023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10366/154065reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1540652026-06-07T06:28:51Z
dc.title.none.fl_str_mv Effect of RONS-Induced Intracellular Redox Homeostasis in 6-NBDG/Glucose Uptake in C2C12 Myotubes and Single Isolated Skeletal Muscle Fibres
title Effect of RONS-Induced Intracellular Redox Homeostasis in 6-NBDG/Glucose Uptake in C2C12 Myotubes and Single Isolated Skeletal Muscle Fibres
spellingShingle Effect of RONS-Induced Intracellular Redox Homeostasis in 6-NBDG/Glucose Uptake in C2C12 Myotubes and Single Isolated Skeletal Muscle Fibres
Martín-Prieto, Eva
Glucose uptake
6-NBDG
insulin resistance
C2C12 myotubes
Skeletal muscle fibres
ROS
Hydrogen peroxide
Nitric oxide
Redox homeostasis
Quantitative fluorescence microscopy
Muscle Fibers, Skeletal
Homeostasis
Glucose
Nitric Oxide
Insulin Resistance
Hydrogen Peroxide
3209 Farmacología
2411.10 Fisiología del Músculo
fibras musculares esqueléticas
peróxido de hidrógeno
óxido nítrico
homeostasis
resistencia a la insulina
glucosa
title_short Effect of RONS-Induced Intracellular Redox Homeostasis in 6-NBDG/Glucose Uptake in C2C12 Myotubes and Single Isolated Skeletal Muscle Fibres
title_full Effect of RONS-Induced Intracellular Redox Homeostasis in 6-NBDG/Glucose Uptake in C2C12 Myotubes and Single Isolated Skeletal Muscle Fibres
title_fullStr Effect of RONS-Induced Intracellular Redox Homeostasis in 6-NBDG/Glucose Uptake in C2C12 Myotubes and Single Isolated Skeletal Muscle Fibres
title_full_unstemmed Effect of RONS-Induced Intracellular Redox Homeostasis in 6-NBDG/Glucose Uptake in C2C12 Myotubes and Single Isolated Skeletal Muscle Fibres
title_sort Effect of RONS-Induced Intracellular Redox Homeostasis in 6-NBDG/Glucose Uptake in C2C12 Myotubes and Single Isolated Skeletal Muscle Fibres
dc.creator.none.fl_str_mv Martín-Prieto, Eva
Márquez, Carlos Manuel
Fernández-Puente, Escarlata
Palomero Labajos, Jesús
author Martín-Prieto, Eva
author_facet Martín-Prieto, Eva
Márquez, Carlos Manuel
Fernández-Puente, Escarlata
Palomero Labajos, Jesús
author_role author
author2 Márquez, Carlos Manuel
Fernández-Puente, Escarlata
Palomero Labajos, Jesús
author2_role author
author
author
dc.subject.none.fl_str_mv Glucose uptake
6-NBDG
insulin resistance
C2C12 myotubes
Skeletal muscle fibres
ROS
Hydrogen peroxide
Nitric oxide
Redox homeostasis
Quantitative fluorescence microscopy
Muscle Fibers, Skeletal
Homeostasis
Glucose
Nitric Oxide
Insulin Resistance
Hydrogen Peroxide
3209 Farmacología
2411.10 Fisiología del Músculo
fibras musculares esqueléticas
peróxido de hidrógeno
óxido nítrico
homeostasis
resistencia a la insulina
glucosa
topic Glucose uptake
6-NBDG
insulin resistance
C2C12 myotubes
Skeletal muscle fibres
ROS
Hydrogen peroxide
Nitric oxide
Redox homeostasis
Quantitative fluorescence microscopy
Muscle Fibers, Skeletal
Homeostasis
Glucose
Nitric Oxide
Insulin Resistance
Hydrogen Peroxide
3209 Farmacología
2411.10 Fisiología del Músculo
fibras musculares esqueléticas
peróxido de hidrógeno
óxido nítrico
homeostasis
resistencia a la insulina
glucosa
description [EN] The glucose uptake in skeletal muscle is essential to produce energy through ATP, which is needed by this organ to maintain vital functions. The impairment of glucose uptake compromises the metabolism and function of skeletal muscle and other organs and is a feature of diabetes, obesity, and ageing. There is a need for research to uncover the mechanisms involved in the impairment of glucose uptake in skeletal muscle. In this study, we adapted, developed, optimised, and validated a methodology based on the fluorescence glucose analogue 6-NBDG, combined with a quantitative fluorescence microscopy image analysis, to determine the glucose uptake in two models of skeletal muscle cells: C2C12 myotubes and single fibres isolated from muscle. It was proposed that reactive oxygen and nitrogen species (RONS) and redox homeostasis play an important role in the modulation of intracellular redox signalling pathways associated with glucose uptake. In this study, we prove that the prooxidative intracellular redox environment under oxidative eustress produced by RONS such as hydrogen peroxide and nitric oxide improves glucose uptake in skeletal muscle cells. However, when oxidation is excessive, oxidative distress occurs, and cellular viability is compromised, although there might be an increase in the glucose uptake. Based on the results of this study, the determination of 6-NBDG/glucose uptake in myotubes and skeletal muscle cells is feasible, validated, and will contribute to improve future research.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/154065
url http://hdl.handle.net/10366/154065
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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