Chemotherapy with phage lysins reduces pneumococcal colonization of the respiratory tract

35 p.-7 fig.-1 tab.

Detalles Bibliográficos
Autores: Corsini, B., Díez-Martínez, Roberto, Aguinagalde, Leire, González-Camacho, Fernando, García-Fernández, Esther, Letrado, Patricia, García González, Pedro, Yuste, José
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/163051
Acceso en línea:http://hdl.handle.net/10261/163051
Access Level:acceso abierto
Palabra clave:Pneumococcus
Phage lysins
Nasopharyngeal colonization
Mouse model
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spelling Chemotherapy with phage lysins reduces pneumococcal colonization of the respiratory tractPhage lysins against pneumococcal colonizationCorsini, B.Díez-Martínez, RobertoAguinagalde, LeireGonzález-Camacho, FernandoGarcía-Fernández, EstherLetrado, PatriciaGarcía González, PedroYuste, JoséPneumococcusPhage lysinsNasopharyngeal colonizationMouse model35 p.-7 fig.-1 tab.Bacteriophage-encoded lytic enzymes, also named lysins, or enzybiotics, are efficient agents to kill bacterial pathogens. Colonization of the respiratory tract by Streptococcus pneumoniae is a prerequisite for the establishment of the infection process. Hence, we have evaluated the antibacterial activity of three different lysins against pneumococcal colonization using human nasopharyngeal and lung epithelial cells as well as a mouse model of nasopharyngeal colonization. The lysins tested were the wild type Cpl-1, the engineered Cpl-7S, and the chimera Cpl-711. Moreover, we have included amoxicillin as a comparator antibiotic. Human epithelial cells were infected with three different multidrug-resistant clinical isolates of S. pneumoniae followed by a single dose of the corresponding lysin. The antimicrobial activity of these lysins was also evaluated using a mouse nasopharyngeal carriage model. Exposure of infected epithelial cells to Cpl-7S did not result in the killing of any of the pneumococcal strains investigated. However, treatment with Cpl-1 or Cpl-711 increased the killing of S. pneumoniae adhered to both types of human epithelial cells with Cpl-711 being more effective than Cpl-1, at sub-inhibitory concentrations. In addition, treatment with amoxicillin had no effect reducing the carrier state whereas mice treated by the intranasal route with Cpl-711 showed significantly reduced nasopharyngeal colonization with no detection of bacterial load in 20-40% of the mice. This study indicates that Cpl-1 and Cpl-711 lysins might be promising antimicrobial candidates for therapy against pneumococcal colonization.This work was supported by grants SAF2017-83388 and SAF2017-88664 from Ministerio de Economía y Competitividad (MINECO)Peer reviewedAmerican Society for MicrobiologyMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201820182018info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/163051reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1128/AAC.02212-17Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1630512026-05-22T06:33:51Z
dc.title.none.fl_str_mv Chemotherapy with phage lysins reduces pneumococcal colonization of the respiratory tract
Phage lysins against pneumococcal colonization
title Chemotherapy with phage lysins reduces pneumococcal colonization of the respiratory tract
spellingShingle Chemotherapy with phage lysins reduces pneumococcal colonization of the respiratory tract
Corsini, B.
Pneumococcus
Phage lysins
Nasopharyngeal colonization
Mouse model
title_short Chemotherapy with phage lysins reduces pneumococcal colonization of the respiratory tract
title_full Chemotherapy with phage lysins reduces pneumococcal colonization of the respiratory tract
title_fullStr Chemotherapy with phage lysins reduces pneumococcal colonization of the respiratory tract
title_full_unstemmed Chemotherapy with phage lysins reduces pneumococcal colonization of the respiratory tract
title_sort Chemotherapy with phage lysins reduces pneumococcal colonization of the respiratory tract
dc.creator.none.fl_str_mv Corsini, B.
Díez-Martínez, Roberto
Aguinagalde, Leire
González-Camacho, Fernando
García-Fernández, Esther
Letrado, Patricia
García González, Pedro
Yuste, José
author Corsini, B.
author_facet Corsini, B.
Díez-Martínez, Roberto
Aguinagalde, Leire
González-Camacho, Fernando
García-Fernández, Esther
Letrado, Patricia
García González, Pedro
Yuste, José
author_role author
author2 Díez-Martínez, Roberto
Aguinagalde, Leire
González-Camacho, Fernando
García-Fernández, Esther
Letrado, Patricia
García González, Pedro
Yuste, José
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Pneumococcus
Phage lysins
Nasopharyngeal colonization
Mouse model
topic Pneumococcus
Phage lysins
Nasopharyngeal colonization
Mouse model
description 35 p.-7 fig.-1 tab.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/163051
url http://hdl.handle.net/10261/163051
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1128/AAC.02212-17
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869409042979356672
score 15,812429

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