Correlations of telomere length, telomeraseactivity, and telomeric-repeat binding factor 1expression in colorectal carcinoma. Prognostic indications

BACKGROUND Telomere maintenance has been proposed as an essential step for tumor cell immortalization. The objectives of the current study were to investigate the mechanisms implicated in telomere length in colorectal carcinoma (CRC) and to evaluate the prognostic impact of telomere status. METHODS...

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Autores: Garcia‐Aranda, Cristina, Diaz‐Lopez, Antonio, Pilar Iniesta, Juan Chocano, María Del Carmen De, Sánchez Pernaute, Andrés, Torres García, Antonio José, Díaz-Rubio García, Eduardo, Balibrea Cantero, José Luis, Benito De Las Heras, Manuel R., Iniesta Serrano, María Pilar
Tipo de recurso: artículo
Fecha de publicación:2005
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/116206
Acceso en línea:https://hdl.handle.net/20.500.14352/116206
Access Level:acceso abierto
Palabra clave:616-006.04
Cáncer
Carcicoma
Cáncer Colorrectal
Ciencias Biomédicas
32 Ciencias Médicas
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repository_id_str
spelling Correlations of telomere length, telomeraseactivity, and telomeric-repeat binding factor 1expression in colorectal carcinoma. Prognostic indicationsGarcia‐Aranda, CristinaDiaz‐Lopez, AntonioPilar IniestaJuan Chocano, María Del Carmen DeSánchez Pernaute, AndrésTorres García, Antonio JoséDíaz-Rubio García, EduardoBalibrea Cantero, José LuisBenito De Las Heras, Manuel R.Iniesta Serrano, María Pilar616-006.04CáncerCarcicomaCáncer ColorrectalCiencias Biomédicas32 Ciencias MédicasBACKGROUND Telomere maintenance has been proposed as an essential step for tumor cell immortalization. The objectives of the current study were to investigate the mechanisms implicated in telomere length in colorectal carcinoma (CRC) and to evaluate the prognostic impact of telomere status. METHODS Ninety‐one colorectal carcinoma samples that were obtained from patients who underwent surgery were analyzed to investigate the factors related to telomere function. The authors studied telomerase activity, terminal restriction fragment (TRF) length, and telomeric‐repeat binding factor (TRF1) expression and analyzed the prognostic implications of those factors. RESULTS Most tumors (81.3%) displayed telomerase activity. Overall, telomeres in CRC specimens were significantly shorter compared with telomeres in normal, adjacent specimens (P = 0.02). Moreover, tumors that demonstrated shortened telomeres displayed higher TRF1 levels than tumors without telomere shortening. In relation to patient prognosis, a significantly poor clinical course was observed in the group of patients who had tumors with longer telomeres (P = 0.02), and this finding emerged as an independent prognostic factor in a Cox proportional hazards model (P = 0.04; relative risk, 6.48). Among patients with tumors classified as telomerase‐positive, telomere length ratios (the ratio of tumor tissues to normal tissues) ≤ 0.66 or TRF1 over‐expression conferred a favorable outcome (P = 0.03 and P = 0.05, respectively). CONCLUSIONS The majority of CRC specimens in the current study displayed telomerase reactivation. However, only those tumors that displayed telomere elongation conferred a poor prognosis. Conversely, colorectal tumors that over‐expressed TRF1 demonstrated telomere shortening, and patients with those tumors had a better clinical course. Cancer 2006. © 2005 American Cancer Society.WILEYUniversidad Complutense de Madrid20052005-12-3020052005-12-30journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/116206reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1162062026-06-02T12:44:21Z
dc.title.none.fl_str_mv Correlations of telomere length, telomeraseactivity, and telomeric-repeat binding factor 1expression in colorectal carcinoma. Prognostic indications
title Correlations of telomere length, telomeraseactivity, and telomeric-repeat binding factor 1expression in colorectal carcinoma. Prognostic indications
spellingShingle Correlations of telomere length, telomeraseactivity, and telomeric-repeat binding factor 1expression in colorectal carcinoma. Prognostic indications
Garcia‐Aranda, Cristina
616-006.04
Cáncer
Carcicoma
Cáncer Colorrectal
Ciencias Biomédicas
32 Ciencias Médicas
title_short Correlations of telomere length, telomeraseactivity, and telomeric-repeat binding factor 1expression in colorectal carcinoma. Prognostic indications
title_full Correlations of telomere length, telomeraseactivity, and telomeric-repeat binding factor 1expression in colorectal carcinoma. Prognostic indications
title_fullStr Correlations of telomere length, telomeraseactivity, and telomeric-repeat binding factor 1expression in colorectal carcinoma. Prognostic indications
title_full_unstemmed Correlations of telomere length, telomeraseactivity, and telomeric-repeat binding factor 1expression in colorectal carcinoma. Prognostic indications
title_sort Correlations of telomere length, telomeraseactivity, and telomeric-repeat binding factor 1expression in colorectal carcinoma. Prognostic indications
dc.creator.none.fl_str_mv Garcia‐Aranda, Cristina
Diaz‐Lopez, Antonio
Pilar Iniesta
Juan Chocano, María Del Carmen De
Sánchez Pernaute, Andrés
Torres García, Antonio José
Díaz-Rubio García, Eduardo
Balibrea Cantero, José Luis
Benito De Las Heras, Manuel R.
Iniesta Serrano, María Pilar
author Garcia‐Aranda, Cristina
author_facet Garcia‐Aranda, Cristina
Diaz‐Lopez, Antonio
Pilar Iniesta
Juan Chocano, María Del Carmen De
Sánchez Pernaute, Andrés
Torres García, Antonio José
Díaz-Rubio García, Eduardo
Balibrea Cantero, José Luis
Benito De Las Heras, Manuel R.
Iniesta Serrano, María Pilar
author_role author
author2 Diaz‐Lopez, Antonio
Pilar Iniesta
Juan Chocano, María Del Carmen De
Sánchez Pernaute, Andrés
Torres García, Antonio José
Díaz-Rubio García, Eduardo
Balibrea Cantero, José Luis
Benito De Las Heras, Manuel R.
Iniesta Serrano, María Pilar
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 616-006.04
Cáncer
Carcicoma
Cáncer Colorrectal
Ciencias Biomédicas
32 Ciencias Médicas
topic 616-006.04
Cáncer
Carcicoma
Cáncer Colorrectal
Ciencias Biomédicas
32 Ciencias Médicas
description BACKGROUND Telomere maintenance has been proposed as an essential step for tumor cell immortalization. The objectives of the current study were to investigate the mechanisms implicated in telomere length in colorectal carcinoma (CRC) and to evaluate the prognostic impact of telomere status. METHODS Ninety‐one colorectal carcinoma samples that were obtained from patients who underwent surgery were analyzed to investigate the factors related to telomere function. The authors studied telomerase activity, terminal restriction fragment (TRF) length, and telomeric‐repeat binding factor (TRF1) expression and analyzed the prognostic implications of those factors. RESULTS Most tumors (81.3%) displayed telomerase activity. Overall, telomeres in CRC specimens were significantly shorter compared with telomeres in normal, adjacent specimens (P = 0.02). Moreover, tumors that demonstrated shortened telomeres displayed higher TRF1 levels than tumors without telomere shortening. In relation to patient prognosis, a significantly poor clinical course was observed in the group of patients who had tumors with longer telomeres (P = 0.02), and this finding emerged as an independent prognostic factor in a Cox proportional hazards model (P = 0.04; relative risk, 6.48). Among patients with tumors classified as telomerase‐positive, telomere length ratios (the ratio of tumor tissues to normal tissues) ≤ 0.66 or TRF1 over‐expression conferred a favorable outcome (P = 0.03 and P = 0.05, respectively). CONCLUSIONS The majority of CRC specimens in the current study displayed telomerase reactivation. However, only those tumors that displayed telomere elongation conferred a poor prognosis. Conversely, colorectal tumors that over‐expressed TRF1 demonstrated telomere shortening, and patients with those tumors had a better clinical course. Cancer 2006. © 2005 American Cancer Society.
publishDate 2005
dc.date.none.fl_str_mv 2005
2005-12-30
2005
2005-12-30
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/116206
url https://hdl.handle.net/20.500.14352/116206
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv WILEY
publisher.none.fl_str_mv WILEY
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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