Functional Impact of the H2A.Z Histone Variant During Meiosis in Saccharomyces cerevisiae

[EN]Among the collection of chromatin modifications that influence its function and structure, the substitution of canonical histones by the so-called histone variants is one of the most prominent actions. Since crucial meiotic transactions are modulated by chromatin, here we investigate the functio...

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Autores: González-Arranz, Sara, Cavero, Santiago, Morillo-Huesca, Macarena, Andújar, Eloisa, Pérez-Alegre, Mónica, Prado, Félix, San-Segundo, Pedro
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/146666
Acceso en línea:http://hdl.handle.net/10366/146666
Access Level:acceso abierto
Palabra clave:Gametogenesis
H2A.Z histone variant
Meiosis
Meiotic recombination checkpoint
Saccharomyces cerevisiae
2415 Biología Molecular
meiosis
gametogénesis
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spelling Functional Impact of the H2A.Z Histone Variant During Meiosis in Saccharomyces cerevisiaeGonzález-Arranz, SaraCavero, SantiagoMorillo-Huesca, MacarenaAndújar, EloisaPérez-Alegre, MónicaPrado, FélixSan-Segundo, PedroGametogenesisH2A.Z histone variantMeiosisMeiotic recombination checkpointSaccharomyces cerevisiaeGametogenesisMeiosisSaccharomyces cerevisiae2415 Biología MolecularmeiosisgametogénesisSaccharomyces cerevisiae[EN]Among the collection of chromatin modifications that influence its function and structure, the substitution of canonical histones by the so-called histone variants is one of the most prominent actions. Since crucial meiotic transactions are modulated by chromatin, here we investigate the functional contribution of the H2A.Z histone variant during both unperturbed meiosis and upon challenging conditions where the meiotic recombination checkpoint is triggered in budding yeast by the absence of the synaptonemal complex component Zip1. We have found that H2A.Z localizes to meiotic chromosomes in an SWR1-dependent manner. Although meiotic recombination is not substantially altered, the htz1 mutant (lacking H2A.Z) shows inefficient meiotic progression, impaired sporulation, and reduced spore viability. These phenotypes are likely accounted for by the misregulation of meiotic gene expression landscape observed in htz1. In the zip1 mutant, the absence of H2A.Z results in a tighter meiotic arrest imposed by the meiotic recombination checkpoint. We have found that Mec1-dependent Hop1-T318 phosphorylation and the ensuing Mek1 activation are not significantly altered in zip1 htz1; however, downstream checkpoint targets, such as the meiosis I-promoting factors Ndt80, Cdc5, and Clb1, are drastically downregulated. The study of the checkpoint response in zip1 htz1 has also allowed us to reveal the existence of an additional function of the Swe1 kinase, independent of CDK inhibitory phosphorylation, which is relevant to restrain meiotic cell cycle progression. In summary, our study shows that the H2A.Z histone variant impacts various aspects of meiotic development adding further insight into the relevance of chromatin dynamics for accurate gametogenesis.Genetics Society of America202120212018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/146666reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1466662026-06-07T06:28:51Z
dc.title.none.fl_str_mv Functional Impact of the H2A.Z Histone Variant During Meiosis in Saccharomyces cerevisiae
title Functional Impact of the H2A.Z Histone Variant During Meiosis in Saccharomyces cerevisiae
spellingShingle Functional Impact of the H2A.Z Histone Variant During Meiosis in Saccharomyces cerevisiae
González-Arranz, Sara
Gametogenesis
H2A.Z histone variant
Meiosis
Meiotic recombination checkpoint
Saccharomyces cerevisiae
Gametogenesis
Meiosis
Saccharomyces cerevisiae
2415 Biología Molecular
meiosis
gametogénesis
Saccharomyces cerevisiae
title_short Functional Impact of the H2A.Z Histone Variant During Meiosis in Saccharomyces cerevisiae
title_full Functional Impact of the H2A.Z Histone Variant During Meiosis in Saccharomyces cerevisiae
title_fullStr Functional Impact of the H2A.Z Histone Variant During Meiosis in Saccharomyces cerevisiae
title_full_unstemmed Functional Impact of the H2A.Z Histone Variant During Meiosis in Saccharomyces cerevisiae
title_sort Functional Impact of the H2A.Z Histone Variant During Meiosis in Saccharomyces cerevisiae
dc.creator.none.fl_str_mv González-Arranz, Sara
Cavero, Santiago
Morillo-Huesca, Macarena
Andújar, Eloisa
Pérez-Alegre, Mónica
Prado, Félix
San-Segundo, Pedro
author González-Arranz, Sara
author_facet González-Arranz, Sara
Cavero, Santiago
Morillo-Huesca, Macarena
Andújar, Eloisa
Pérez-Alegre, Mónica
Prado, Félix
San-Segundo, Pedro
author_role author
author2 Cavero, Santiago
Morillo-Huesca, Macarena
Andújar, Eloisa
Pérez-Alegre, Mónica
Prado, Félix
San-Segundo, Pedro
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Gametogenesis
H2A.Z histone variant
Meiosis
Meiotic recombination checkpoint
Saccharomyces cerevisiae
Gametogenesis
Meiosis
Saccharomyces cerevisiae
2415 Biología Molecular
meiosis
gametogénesis
Saccharomyces cerevisiae
topic Gametogenesis
H2A.Z histone variant
Meiosis
Meiotic recombination checkpoint
Saccharomyces cerevisiae
Gametogenesis
Meiosis
Saccharomyces cerevisiae
2415 Biología Molecular
meiosis
gametogénesis
Saccharomyces cerevisiae
description [EN]Among the collection of chromatin modifications that influence its function and structure, the substitution of canonical histones by the so-called histone variants is one of the most prominent actions. Since crucial meiotic transactions are modulated by chromatin, here we investigate the functional contribution of the H2A.Z histone variant during both unperturbed meiosis and upon challenging conditions where the meiotic recombination checkpoint is triggered in budding yeast by the absence of the synaptonemal complex component Zip1. We have found that H2A.Z localizes to meiotic chromosomes in an SWR1-dependent manner. Although meiotic recombination is not substantially altered, the htz1 mutant (lacking H2A.Z) shows inefficient meiotic progression, impaired sporulation, and reduced spore viability. These phenotypes are likely accounted for by the misregulation of meiotic gene expression landscape observed in htz1. In the zip1 mutant, the absence of H2A.Z results in a tighter meiotic arrest imposed by the meiotic recombination checkpoint. We have found that Mec1-dependent Hop1-T318 phosphorylation and the ensuing Mek1 activation are not significantly altered in zip1 htz1; however, downstream checkpoint targets, such as the meiosis I-promoting factors Ndt80, Cdc5, and Clb1, are drastically downregulated. The study of the checkpoint response in zip1 htz1 has also allowed us to reveal the existence of an additional function of the Swe1 kinase, independent of CDK inhibitory phosphorylation, which is relevant to restrain meiotic cell cycle progression. In summary, our study shows that the H2A.Z histone variant impacts various aspects of meiotic development adding further insight into the relevance of chromatin dynamics for accurate gametogenesis.
publishDate 2018
dc.date.none.fl_str_mv 2018
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/146666
url http://hdl.handle.net/10366/146666
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Genetics Society of America
publisher.none.fl_str_mv Genetics Society of America
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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