Cutaneous excreta of the earthworm Eisenia fetida (Haplotaxida: Lumbricidae) might hinder the biological control performance of entomopathogenic nematodes

Earthworms enhance soil structure, the decomposition of organic matter and the dissemination of beneficial soil organisms such as the entomopathogenic nematodes (EPNs). Nevertheless, the effects of earthworm feeding behavior or cutaneous excreta (CEx) on the performance of EPNs as biological control...

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Autores: Chelkha, Maryam, Blanco-Pérez, Rubén, Bueno-Pallero, Francisco Ángel, Amghar, Souad, El Harti, Abdellatif, Campos-Herrera, Raquel
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/223496
Acceso en línea:http://hdl.handle.net/10261/223496
Access Level:acceso abierto
Palabra clave:Cutaneous mucus
Eisenia fetida
Heterorhabditis spp.
Steinernema spp.
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spelling Cutaneous excreta of the earthworm Eisenia fetida (Haplotaxida: Lumbricidae) might hinder the biological control performance of entomopathogenic nematodesChelkha, MaryamBlanco-Pérez, RubénBueno-Pallero, Francisco ÁngelAmghar, SouadEl Harti, AbdellatifCampos-Herrera, RaquelCutaneous mucusEisenia fetidaHeterorhabditis spp.Steinernema spp.Earthworms enhance soil structure, the decomposition of organic matter and the dissemination of beneficial soil organisms such as the entomopathogenic nematodes (EPNs). Nevertheless, the effects of earthworm feeding behavior or cutaneous excreta (CEx) on the performance of EPNs as biological control agents is poorly understood. We hypothesized that the presence of earthworms or their excreta reduces EPN fitness, measured in terms of pathogenicity and reproductive success. In laboratory experiments we first evaluated the killing ability of EPNs against Galleria mellonella (Lepidoptera: Pyralidae) larvae when inoculated in autoclaved soil alone or in combination with the earthworm Eisenia fetida (Haplotaxida: Lumbricidae) or their excreta. We also evaluated EPN efficacy and reproduction when exposed to CEx derived from E. fetida at two nematode concentrations (1.5 and 10 IJs/cm2). For both experiments, we tested four steinernematids (Steinernema carpocapsae, S. feltiae, S. glaseri, and S. khuongi) and two heterorhabditids (Heterorhabditis bacteriophora and H. zealandica). The presence of earthworms or their excreta resulted in significant reduction of the larval mortality caused by some of the steinernematids at certain timings depending of the species (P < 0.015), while heterorhabditids were mainly not affected. Both S. feltiae and H. zealandica progeny production was significantly reduced (P < 0.01) when exposed to CEx. Hence, we showed that the presence of CEx might alter the biocontrol performance of certain EPN species, especially steinernematids bigger than 600 μm in size (S. feltiae, S. glaseri, and S. khoungi), by affecting their pathogenicity and reproductive success.The Government of Portugal (FCT), thanks to the “Starting Grant” associate funds (grant number IF/00552/2014/CP1234/CT0007), supported this work. RCH was awarded an Investigator Programme contract (grant number IF/00552/2014). Similarly, FABP and RBP were financed by the scientific assistantship fellowships associated with this grant (BI, UAlg-2016/004 and UAlg-2016/003, respectively, Universidade do Algarve, Portugal). MC was supported as visitor student at the Universidade do Algarve (Portugal) thanks to the international mobility program “Mare-Nostrum”. Currently, MC is supported by a Moroccan scholarship for the Ministry of National Education, Vocational Training, Higher Education and Scientific Research, RBP is financed by the Department of Economic Development and Innovation, Government of La Rioja (Spain) with a predoctoral contract (CAR-2018), and RCH is awarded by Ramon y Cajal contract award (RYC-2016-19939) from the Government of Spain. This study is linked to the CSIC i-coop+ 2018 grant (COOPA20231).Peer reviewedElsevierFundação para a Ciência e a Tecnologia (Portugal)Universidade do AlgarveMinistère de l’Education Nationale, de la Formation Professionnelle, de l'Enseignement Supérieur et de la Recherche Scientifique (Maroc)Gobierno de La RiojaMinisterio de Economía y Competitividad (España)Campos-Herrera, R. [0000-0003-0852-5269]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/223496reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RYC-2016-19939http://dx.doi.org/10.1016/j.soilbio.2019.107691Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2234962026-05-22T06:33:51Z
dc.title.none.fl_str_mv Cutaneous excreta of the earthworm Eisenia fetida (Haplotaxida: Lumbricidae) might hinder the biological control performance of entomopathogenic nematodes
title Cutaneous excreta of the earthworm Eisenia fetida (Haplotaxida: Lumbricidae) might hinder the biological control performance of entomopathogenic nematodes
spellingShingle Cutaneous excreta of the earthworm Eisenia fetida (Haplotaxida: Lumbricidae) might hinder the biological control performance of entomopathogenic nematodes
Chelkha, Maryam
Cutaneous mucus
Eisenia fetida
Heterorhabditis spp.
Steinernema spp.
title_short Cutaneous excreta of the earthworm Eisenia fetida (Haplotaxida: Lumbricidae) might hinder the biological control performance of entomopathogenic nematodes
title_full Cutaneous excreta of the earthworm Eisenia fetida (Haplotaxida: Lumbricidae) might hinder the biological control performance of entomopathogenic nematodes
title_fullStr Cutaneous excreta of the earthworm Eisenia fetida (Haplotaxida: Lumbricidae) might hinder the biological control performance of entomopathogenic nematodes
title_full_unstemmed Cutaneous excreta of the earthworm Eisenia fetida (Haplotaxida: Lumbricidae) might hinder the biological control performance of entomopathogenic nematodes
title_sort Cutaneous excreta of the earthworm Eisenia fetida (Haplotaxida: Lumbricidae) might hinder the biological control performance of entomopathogenic nematodes
dc.creator.none.fl_str_mv Chelkha, Maryam
Blanco-Pérez, Rubén
Bueno-Pallero, Francisco Ángel
Amghar, Souad
El Harti, Abdellatif
Campos-Herrera, Raquel
author Chelkha, Maryam
author_facet Chelkha, Maryam
Blanco-Pérez, Rubén
Bueno-Pallero, Francisco Ángel
Amghar, Souad
El Harti, Abdellatif
Campos-Herrera, Raquel
author_role author
author2 Blanco-Pérez, Rubén
Bueno-Pallero, Francisco Ángel
Amghar, Souad
El Harti, Abdellatif
Campos-Herrera, Raquel
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Fundação para a Ciência e a Tecnologia (Portugal)
Universidade do Algarve
Ministère de l’Education Nationale, de la Formation Professionnelle, de l'Enseignement Supérieur et de la Recherche Scientifique (Maroc)
Gobierno de La Rioja
Ministerio de Economía y Competitividad (España)
Campos-Herrera, R. [0000-0003-0852-5269]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Cutaneous mucus
Eisenia fetida
Heterorhabditis spp.
Steinernema spp.
topic Cutaneous mucus
Eisenia fetida
Heterorhabditis spp.
Steinernema spp.
description Earthworms enhance soil structure, the decomposition of organic matter and the dissemination of beneficial soil organisms such as the entomopathogenic nematodes (EPNs). Nevertheless, the effects of earthworm feeding behavior or cutaneous excreta (CEx) on the performance of EPNs as biological control agents is poorly understood. We hypothesized that the presence of earthworms or their excreta reduces EPN fitness, measured in terms of pathogenicity and reproductive success. In laboratory experiments we first evaluated the killing ability of EPNs against Galleria mellonella (Lepidoptera: Pyralidae) larvae when inoculated in autoclaved soil alone or in combination with the earthworm Eisenia fetida (Haplotaxida: Lumbricidae) or their excreta. We also evaluated EPN efficacy and reproduction when exposed to CEx derived from E. fetida at two nematode concentrations (1.5 and 10 IJs/cm2). For both experiments, we tested four steinernematids (Steinernema carpocapsae, S. feltiae, S. glaseri, and S. khuongi) and two heterorhabditids (Heterorhabditis bacteriophora and H. zealandica). The presence of earthworms or their excreta resulted in significant reduction of the larval mortality caused by some of the steinernematids at certain timings depending of the species (P < 0.015), while heterorhabditids were mainly not affected. Both S. feltiae and H. zealandica progeny production was significantly reduced (P < 0.01) when exposed to CEx. Hence, we showed that the presence of CEx might alter the biocontrol performance of certain EPN species, especially steinernematids bigger than 600 μm in size (S. feltiae, S. glaseri, and S. khoungi), by affecting their pathogenicity and reproductive success.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/223496
url http://hdl.handle.net/10261/223496
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RYC-2016-19939
http://dx.doi.org/10.1016/j.soilbio.2019.107691
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
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collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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