A recombinant subviral particle-based vaccine protects magpie (Pica pica) against west Nile virus infection

The mosquito-borne West Nile virus (WNV) is a highly neurovirulent Flavivirus currently representing an emergent zoonotic concern. WNV cycles in nature between mosquito vectors and birds that act as amplifier hosts and play an essential role in virus ecology, being, thus, WNV a threat to many specie...

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Autores: Jiménez de Oya, Nereida, Escribano-Romero, Estela, Camacho, MariaCruz, Blázquez, Ana B., Martín-Acebes, M. A., Höfle, Ursula, Saiz Calahorra, Juan Carlos
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/215372
Acesso em linha:http://hdl.handle.net/10261/215372
Access Level:acceso abierto
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spelling A recombinant subviral particle-based vaccine protects magpie (Pica pica) against west Nile virus infectionJiménez de Oya, NereidaEscribano-Romero, EstelaCamacho, MariaCruzBlázquez, Ana B.Martín-Acebes, M. A.Höfle, UrsulaSaiz Calahorra, Juan CarlosThe mosquito-borne West Nile virus (WNV) is a highly neurovirulent Flavivirus currently representing an emergent zoonotic concern. WNV cycles in nature between mosquito vectors and birds that act as amplifier hosts and play an essential role in virus ecology, being, thus, WNV a threat to many species. Availability of an efficient avian vaccine would benefit certain avian populations, both birds grown for hunting and restocking activities, as well as endangered species in captive breeding projects, wildlife reservations, and recreation installations, and would be useful to prevent and contain outbreaks. Avian vaccination would be also of interest to limit WNV spillover to humans or horses from susceptible bird species that live in urbanized landscapes, like magpies. Herein, we have addressed the efficacy of a single dose of a WNV recombinant subviral particle (RSP) vaccine in susceptible magpie (Pica pica). The protective capacity of the RSP-based vaccine was demonstrated upon challenge of magpies with 5 × 103 plaque forming units of a neurovirulent WNV strain. A significant improvement in survival rates of immunized birds was recorded when compared to vehicle-inoculated animals (71.4 vs. 22.2%, respectively). Viremia, which is directly related to the capacity of a host to be competent for virus transmission, was reduced in vaccinated animals, as was the presence of infectious virus in feather follicles. Bird-to-bird transmission was recorded in three of six unchallenged (contact) magpies housed with non-vaccinated WNV-infected birds, but not in contact animals housed with vaccinated WNV-infected magpies. These results demonstrate the protective efficacy of the RSP-based vaccine in susceptible birds against WNV infection and its value in controlling the spread of the virus.This work was supported by grants RTA2015-009 and E-RTA20017-003-C02 from INIA, and P2013/ABI-2906 and P2018/BAA-4370 from the Comunidad Autónoma de Madrid.Peer reviewedFrontiers MediaComunidad de MadridCSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/215372reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#P2013/ABI-2906/PLATESA-CMP2018/BAA-4370/PLATESA2-CMhttps://doi.org/10.3389/fmicb.2019.01133Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2153722026-05-22T06:33:51Z
dc.title.none.fl_str_mv A recombinant subviral particle-based vaccine protects magpie (Pica pica) against west Nile virus infection
title A recombinant subviral particle-based vaccine protects magpie (Pica pica) against west Nile virus infection
spellingShingle A recombinant subviral particle-based vaccine protects magpie (Pica pica) against west Nile virus infection
Jiménez de Oya, Nereida
title_short A recombinant subviral particle-based vaccine protects magpie (Pica pica) against west Nile virus infection
title_full A recombinant subviral particle-based vaccine protects magpie (Pica pica) against west Nile virus infection
title_fullStr A recombinant subviral particle-based vaccine protects magpie (Pica pica) against west Nile virus infection
title_full_unstemmed A recombinant subviral particle-based vaccine protects magpie (Pica pica) against west Nile virus infection
title_sort A recombinant subviral particle-based vaccine protects magpie (Pica pica) against west Nile virus infection
dc.creator.none.fl_str_mv Jiménez de Oya, Nereida
Escribano-Romero, Estela
Camacho, MariaCruz
Blázquez, Ana B.
Martín-Acebes, M. A.
Höfle, Ursula
Saiz Calahorra, Juan Carlos
author Jiménez de Oya, Nereida
author_facet Jiménez de Oya, Nereida
Escribano-Romero, Estela
Camacho, MariaCruz
Blázquez, Ana B.
Martín-Acebes, M. A.
Höfle, Ursula
Saiz Calahorra, Juan Carlos
author_role author
author2 Escribano-Romero, Estela
Camacho, MariaCruz
Blázquez, Ana B.
Martín-Acebes, M. A.
Höfle, Ursula
Saiz Calahorra, Juan Carlos
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Comunidad de Madrid
CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description The mosquito-borne West Nile virus (WNV) is a highly neurovirulent Flavivirus currently representing an emergent zoonotic concern. WNV cycles in nature between mosquito vectors and birds that act as amplifier hosts and play an essential role in virus ecology, being, thus, WNV a threat to many species. Availability of an efficient avian vaccine would benefit certain avian populations, both birds grown for hunting and restocking activities, as well as endangered species in captive breeding projects, wildlife reservations, and recreation installations, and would be useful to prevent and contain outbreaks. Avian vaccination would be also of interest to limit WNV spillover to humans or horses from susceptible bird species that live in urbanized landscapes, like magpies. Herein, we have addressed the efficacy of a single dose of a WNV recombinant subviral particle (RSP) vaccine in susceptible magpie (Pica pica). The protective capacity of the RSP-based vaccine was demonstrated upon challenge of magpies with 5 × 103 plaque forming units of a neurovirulent WNV strain. A significant improvement in survival rates of immunized birds was recorded when compared to vehicle-inoculated animals (71.4 vs. 22.2%, respectively). Viremia, which is directly related to the capacity of a host to be competent for virus transmission, was reduced in vaccinated animals, as was the presence of infectious virus in feather follicles. Bird-to-bird transmission was recorded in three of six unchallenged (contact) magpies housed with non-vaccinated WNV-infected birds, but not in contact animals housed with vaccinated WNV-infected magpies. These results demonstrate the protective efficacy of the RSP-based vaccine in susceptible birds against WNV infection and its value in controlling the spread of the virus.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/215372
url http://hdl.handle.net/10261/215372
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
P2013/ABI-2906/PLATESA-CM
P2018/BAA-4370/PLATESA2-CM
https://doi.org/10.3389/fmicb.2019.01133
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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