A Novel Prototype African Swine Fever Virus DIVA (Differentiation Between Infected and Vaccinated Animals) Serological Assay Based on the Detection of Antibodies Against the pEP153R, eGFP, and p72 Proteins

This article belongs to the Special Issue Vaccine Development for Swine Viral Pathogens.

Detalles Bibliográficos
Autores: González García, Gabriela, Gallardo, Carmina, Montón, Mercedes, Barroso Arévalo, Sandra, Casado, Nadia, Barasona, José A., Sánchez-Vizcaíno, J. M., Venteo, Ángel, Sastre, Patricia, Rueda, Paloma
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/382514
Acceso en línea:http://hdl.handle.net/10261/382514
Access Level:acceso abierto
Palabra clave:ASF
ASFV
DIVA
Diagnosis
pEP153R
C-type lectin protein
eGFP
Vaccine
ELISA
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spelling A Novel Prototype African Swine Fever Virus DIVA (Differentiation Between Infected and Vaccinated Animals) Serological Assay Based on the Detection of Antibodies Against the pEP153R, eGFP, and p72 ProteinsGonzález García, GabrielaGallardo, CarminaMontón, MercedesBarroso Arévalo, SandraCasado, NadiaBarasona, José A.Sánchez-Vizcaíno, J. M.Venteo, ÁngelSastre, PatriciaRueda, PalomaASFASFVDIVADiagnosispEP153RC-type lectin proteineGFPVaccineELISAThis article belongs to the Special Issue Vaccine Development for Swine Viral Pathogens.[Background/Objectives] African Swine Fever (ASF) is one of the most significant infectious diseases affecting both domestic pig and wild boar populations, leading to substantial economic and biosanitary consequences. In Europe, disease management relies on stringent biosecurity measures and surveillance through diagnosis, highlighting the urgent need for an effective and safe vaccine for ASF control. In this context, the VACDIVA project has generated several promising vaccine candidates, including those with the EP153R gene deleted and replaced by the eGFP reporter gene.[Methods] In this study, pEP153R and eGFP proteins were produced using recombinant technology and demonstrated their antigenicity and DIVA capability through indirect ELISA. Additionally, a prototype serological DIVA test was designed and developed. The assay is based on the detection of antibodies against both DIVA antigens and the well-established immunogenic p72 protein.[Conclusions] This approach could potentially improve surveillance during prospective vaccination campaigns.This study was funded by European Union’s Horizon 2020 research and innovation programme under grant agreement number 862874 (VACDIVA: A Safe DIVA vaccine for African Swine Fever control and eradication). G.G.-G. is a recipient of grant DIN2019-010833/AEI/10.13039/501100011033 funded by the Spanish Ministry of Science, Innovation and Universities (Ministerio de Ciencia, Innovación y Universidades/Agencia Estatal de Investigación). J.Á.B. is a recipient of a ’Ramón y Cajal’ contract [RYC2022-038060-I] funded by the Spanish Ministry of Science and Innovation (MCIN/AEI) and Fondo Social Europeo Plus (FSE+).Peer reviewedMultidisciplinary Digital Publishing InstituteEuropean CommissionMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2025202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/382514reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/862874The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/vaccines13030211https://doi.org/10.3390/vaccines13030211Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3825142026-05-22T06:33:51Z
dc.title.none.fl_str_mv A Novel Prototype African Swine Fever Virus DIVA (Differentiation Between Infected and Vaccinated Animals) Serological Assay Based on the Detection of Antibodies Against the pEP153R, eGFP, and p72 Proteins
title A Novel Prototype African Swine Fever Virus DIVA (Differentiation Between Infected and Vaccinated Animals) Serological Assay Based on the Detection of Antibodies Against the pEP153R, eGFP, and p72 Proteins
spellingShingle A Novel Prototype African Swine Fever Virus DIVA (Differentiation Between Infected and Vaccinated Animals) Serological Assay Based on the Detection of Antibodies Against the pEP153R, eGFP, and p72 Proteins
González García, Gabriela
ASF
ASFV
DIVA
Diagnosis
pEP153R
C-type lectin protein
eGFP
Vaccine
ELISA
title_short A Novel Prototype African Swine Fever Virus DIVA (Differentiation Between Infected and Vaccinated Animals) Serological Assay Based on the Detection of Antibodies Against the pEP153R, eGFP, and p72 Proteins
title_full A Novel Prototype African Swine Fever Virus DIVA (Differentiation Between Infected and Vaccinated Animals) Serological Assay Based on the Detection of Antibodies Against the pEP153R, eGFP, and p72 Proteins
title_fullStr A Novel Prototype African Swine Fever Virus DIVA (Differentiation Between Infected and Vaccinated Animals) Serological Assay Based on the Detection of Antibodies Against the pEP153R, eGFP, and p72 Proteins
title_full_unstemmed A Novel Prototype African Swine Fever Virus DIVA (Differentiation Between Infected and Vaccinated Animals) Serological Assay Based on the Detection of Antibodies Against the pEP153R, eGFP, and p72 Proteins
title_sort A Novel Prototype African Swine Fever Virus DIVA (Differentiation Between Infected and Vaccinated Animals) Serological Assay Based on the Detection of Antibodies Against the pEP153R, eGFP, and p72 Proteins
dc.creator.none.fl_str_mv González García, Gabriela
Gallardo, Carmina
Montón, Mercedes
Barroso Arévalo, Sandra
Casado, Nadia
Barasona, José A.
Sánchez-Vizcaíno, J. M.
Venteo, Ángel
Sastre, Patricia
Rueda, Paloma
author González García, Gabriela
author_facet González García, Gabriela
Gallardo, Carmina
Montón, Mercedes
Barroso Arévalo, Sandra
Casado, Nadia
Barasona, José A.
Sánchez-Vizcaíno, J. M.
Venteo, Ángel
Sastre, Patricia
Rueda, Paloma
author_role author
author2 Gallardo, Carmina
Montón, Mercedes
Barroso Arévalo, Sandra
Casado, Nadia
Barasona, José A.
Sánchez-Vizcaíno, J. M.
Venteo, Ángel
Sastre, Patricia
Rueda, Paloma
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv ASF
ASFV
DIVA
Diagnosis
pEP153R
C-type lectin protein
eGFP
Vaccine
ELISA
topic ASF
ASFV
DIVA
Diagnosis
pEP153R
C-type lectin protein
eGFP
Vaccine
ELISA
description This article belongs to the Special Issue Vaccine Development for Swine Viral Pathogens.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/382514
url http://hdl.handle.net/10261/382514
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/862874
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/vaccines13030211
https://doi.org/10.3390/vaccines13030211
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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