Nucleosome destabilization by polyamines

The roles and molecular interactions of polyamines (PAs) in the nucleus are not fully understood. Here their effect on nucleosome stability, a key regulatory factor in eukaryotic gene control, is reported, as measured in agarose embedded nuclei of H2B-GFP expressor HeLa cells. Nucleosome stability w...

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Autores: Imre, Laszlo, Firouzi Niaki, Erfaneh, Bosire, Rosevalentine, Nanasi Jr., Peter, Nagy, Peter, Bacso, Zsolt, Hamidova, Nubar, Pommier, Yves, Jordan, Albert, Szabo, Gabor
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/281191
Acceso en línea:http://hdl.handle.net/10261/281191
Access Level:acceso abierto
Palabra clave:Polyamines
Chromatin
Nucleosome
Stability
NCI-60
Histone
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spelling Nucleosome destabilization by polyaminesImre, LaszloFirouzi Niaki, ErfanehBosire, RosevalentineNanasi Jr., PeterNagy, PeterBacso, ZsoltHamidova, NubarPommier, YvesJordan, AlbertSzabo, GaborPolyaminesChromatinNucleosomeStabilityNCI-60HistoneThe roles and molecular interactions of polyamines (PAs) in the nucleus are not fully understood. Here their effect on nucleosome stability, a key regulatory factor in eukaryotic gene control, is reported, as measured in agarose embedded nuclei of H2B-GFP expressor HeLa cells. Nucleosome stability was assessed by quantitative microscopy [[1], [2]] in situ, in close to native state of chromatin, preserving the nucleosome constrained topology of the genomic DNA. A robust destabilizing effect was observed in the millimolar concentration range in the case of spermine, spermidine as well as putrescine, which was strongly pH and salt concentration-dependent, and remained significant also at neutral pH. The integrity of genomic DNA was not affected by PA treatment, excluding DNA break-elicited topological relaxation as a factor in destabilization. The binding of PAs to DNA was demonstrated by the displacement of ethidium bromide, both from deproteinized nuclear halos and from plasmid DNA. The possibility that DNA methylation patterns may be influenced by PA levels is contemplated in the context of gene expression and DNA methylation correlations identified in the NCI-60 panel-based CellMiner database: methylated loci in subsets of high-ODC1 cell lines and the dependence of PER3 DNA methylation on PA metabolism.This work was supported by OTKA K 128770, K 138524, COST CA 15214, Stipendium Hungaricum scholarship by the Tempus Public Foundation (to RB) and the Richter Talentum Fund (EFN and NH). We are thankful to the NIH Developmental Therapeutics Program for providing the drugs investigated.ElsevierTempus Public FoundationNational Institutes of Health (US)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/281191reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésImre, Laszlo; Firouzi Niaki, Erfaneh; Bosire, Rosevalentine; Nanasi Jr., Peter; Nagy, Peter; Bacso, Zsolt; Hamidova, Nubar; Pommier, Yves; Jordan, Albert; Szabo, Gabor; 2022; Nucleosome destabilization by polyamines [Dataset]; Elsevier; http://dx.doi.org/10.1016/j.abb.2022.109184http://dx.doi.org/10.1016/j.abb.2022.109184Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2811912026-05-22T06:33:51Z
dc.title.none.fl_str_mv Nucleosome destabilization by polyamines
title Nucleosome destabilization by polyamines
spellingShingle Nucleosome destabilization by polyamines
Imre, Laszlo
Polyamines
Chromatin
Nucleosome
Stability
NCI-60
Histone
title_short Nucleosome destabilization by polyamines
title_full Nucleosome destabilization by polyamines
title_fullStr Nucleosome destabilization by polyamines
title_full_unstemmed Nucleosome destabilization by polyamines
title_sort Nucleosome destabilization by polyamines
dc.creator.none.fl_str_mv Imre, Laszlo
Firouzi Niaki, Erfaneh
Bosire, Rosevalentine
Nanasi Jr., Peter
Nagy, Peter
Bacso, Zsolt
Hamidova, Nubar
Pommier, Yves
Jordan, Albert
Szabo, Gabor
author Imre, Laszlo
author_facet Imre, Laszlo
Firouzi Niaki, Erfaneh
Bosire, Rosevalentine
Nanasi Jr., Peter
Nagy, Peter
Bacso, Zsolt
Hamidova, Nubar
Pommier, Yves
Jordan, Albert
Szabo, Gabor
author_role author
author2 Firouzi Niaki, Erfaneh
Bosire, Rosevalentine
Nanasi Jr., Peter
Nagy, Peter
Bacso, Zsolt
Hamidova, Nubar
Pommier, Yves
Jordan, Albert
Szabo, Gabor
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Tempus Public Foundation
National Institutes of Health (US)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Polyamines
Chromatin
Nucleosome
Stability
NCI-60
Histone
topic Polyamines
Chromatin
Nucleosome
Stability
NCI-60
Histone
description The roles and molecular interactions of polyamines (PAs) in the nucleus are not fully understood. Here their effect on nucleosome stability, a key regulatory factor in eukaryotic gene control, is reported, as measured in agarose embedded nuclei of H2B-GFP expressor HeLa cells. Nucleosome stability was assessed by quantitative microscopy [[1], [2]] in situ, in close to native state of chromatin, preserving the nucleosome constrained topology of the genomic DNA. A robust destabilizing effect was observed in the millimolar concentration range in the case of spermine, spermidine as well as putrescine, which was strongly pH and salt concentration-dependent, and remained significant also at neutral pH. The integrity of genomic DNA was not affected by PA treatment, excluding DNA break-elicited topological relaxation as a factor in destabilization. The binding of PAs to DNA was demonstrated by the displacement of ethidium bromide, both from deproteinized nuclear halos and from plasmid DNA. The possibility that DNA methylation patterns may be influenced by PA levels is contemplated in the context of gene expression and DNA methylation correlations identified in the NCI-60 panel-based CellMiner database: methylated loci in subsets of high-ODC1 cell lines and the dependence of PER3 DNA methylation on PA metabolism.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/281191
url http://hdl.handle.net/10261/281191
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Imre, Laszlo; Firouzi Niaki, Erfaneh; Bosire, Rosevalentine; Nanasi Jr., Peter; Nagy, Peter; Bacso, Zsolt; Hamidova, Nubar; Pommier, Yves; Jordan, Albert; Szabo, Gabor; 2022; Nucleosome destabilization by polyamines [Dataset]; Elsevier; http://dx.doi.org/10.1016/j.abb.2022.109184
http://dx.doi.org/10.1016/j.abb.2022.109184
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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