Transient Nanoscopic Phase Separation in Biological Lipid Membranes Resolved by Planar Plasmonic Antennas

Nanoscale membrane assemblies of sphingolipids, cholesterol, and certain proteins, also known as lipid rafts, play a crucial role in facilitating a broad range of important cell functions. Whereas on living cell membranes lipid rafts have been postulated to have nanoscopic dimensions and to be highl...

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Autores: Winkler, Pamina M., Regmi, Raju, Flauraud, Valentin, Brugger, Jürgen, Rigneault, Hervé, Wenger, Jérôme, Garcia-Parajo, Maria F.
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/172443
Acceso en línea:https://hdl.handle.net/2117/172443
https://dx.doi.org/10.1021/acsnano.7b03177
Access Level:acceso abierto
Palabra clave:Antennas (Electronics)
antennas
Antenes (Electrònica)
Àrees temàtiques de la UPC::Física
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spelling Transient Nanoscopic Phase Separation in Biological Lipid Membranes Resolved by Planar Plasmonic AntennasWinkler, Pamina M.Regmi, RajuFlauraud, ValentinBrugger, JürgenRigneault, HervéWenger, JérômeGarcia-Parajo, Maria F.Antennas (Electronics)antennasAntenes (Electrònica)Àrees temàtiques de la UPC::FísicaNanoscale membrane assemblies of sphingolipids, cholesterol, and certain proteins, also known as lipid rafts, play a crucial role in facilitating a broad range of important cell functions. Whereas on living cell membranes lipid rafts have been postulated to have nanoscopic dimensions and to be highly transient, the existence of a similar type of dynamic nanodomains in multicomponent lipid bilayers has been questioned. Here, we perform fluorescence correlation spectroscopy on planar plasmonic antenna arrays with different nanogap sizes to assess the dynamic nanoscale organization of mimetic biological membranes. Our approach takes advantage of the highly enhanced and confined excitation light provided by the nanoantennas together with their outstanding planarity to investigate membrane regions as small as 10 nm in size with microsecond time resolution. Our diffusion data are consistent with the coexistence of transient nanoscopic domains in both the liquid-ordered and the liquid-disordered microscopic phases of multicomponent lipid bilayers. These nanodomains have characteristic residence times between 30 and 150 μs and sizes around 10 nm, as inferred from the diffusion data. Thus, although microscale phase separation occurs on mimetic membranes, nanoscopic domains also coexist, suggesting that these transient assemblies might be similar to those occurring in living cells, which in the absence of raft-stabilizing proteins are poised to be short-lived. Importantly, our work underscores the high potential of photonic nanoantennas to interrogate the nanoscale heterogeneity of native biological membranes with ultrahigh spatiotemporal resolution.Peer ReviewedACS20172017-07-1120182018-01-11journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/172443https://dx.doi.org/10.1021/acsnano.7b03177reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)InglésengEuropean Commission http://doi.org/10.13039/100010661 Horizon 2020 Framework Programme 665884 ICFOstepstone PhD Programme for Early-Stage Researchers in PhotonicsEuropean Commission http://dx.doi.org/10.13039/100011102 Seventh Framework Programme 288263 Advanced photonic antenna tools for biosensing and cellular nanoimagingEuropean Commission http://dx.doi.org/10.13039/100011102 Seventh Framework Programme 278242 Extended fluorescence resonance energy transfer with plasmonic nanocircuitsEuropean Commission http://doi.org/10.13039/100010661 Horizon 2020 Framework Programme 713729 ICFOstepstone PhD Programme for Early-Stage Researchers in Photonicsopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivs 3.0 Spainhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/1724432026-05-27T15:37:01Z
dc.title.none.fl_str_mv Transient Nanoscopic Phase Separation in Biological Lipid Membranes Resolved by Planar Plasmonic Antennas
title Transient Nanoscopic Phase Separation in Biological Lipid Membranes Resolved by Planar Plasmonic Antennas
spellingShingle Transient Nanoscopic Phase Separation in Biological Lipid Membranes Resolved by Planar Plasmonic Antennas
Winkler, Pamina M.
Antennas (Electronics)
antennas
Antenes (Electrònica)
Àrees temàtiques de la UPC::Física
title_short Transient Nanoscopic Phase Separation in Biological Lipid Membranes Resolved by Planar Plasmonic Antennas
title_full Transient Nanoscopic Phase Separation in Biological Lipid Membranes Resolved by Planar Plasmonic Antennas
title_fullStr Transient Nanoscopic Phase Separation in Biological Lipid Membranes Resolved by Planar Plasmonic Antennas
title_full_unstemmed Transient Nanoscopic Phase Separation in Biological Lipid Membranes Resolved by Planar Plasmonic Antennas
title_sort Transient Nanoscopic Phase Separation in Biological Lipid Membranes Resolved by Planar Plasmonic Antennas
dc.creator.none.fl_str_mv Winkler, Pamina M.
Regmi, Raju
Flauraud, Valentin
Brugger, Jürgen
Rigneault, Hervé
Wenger, Jérôme
Garcia-Parajo, Maria F.
author Winkler, Pamina M.
author_facet Winkler, Pamina M.
Regmi, Raju
Flauraud, Valentin
Brugger, Jürgen
Rigneault, Hervé
Wenger, Jérôme
Garcia-Parajo, Maria F.
author_role author
author2 Regmi, Raju
Flauraud, Valentin
Brugger, Jürgen
Rigneault, Hervé
Wenger, Jérôme
Garcia-Parajo, Maria F.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Antennas (Electronics)
antennas
Antenes (Electrònica)
Àrees temàtiques de la UPC::Física
topic Antennas (Electronics)
antennas
Antenes (Electrònica)
Àrees temàtiques de la UPC::Física
description Nanoscale membrane assemblies of sphingolipids, cholesterol, and certain proteins, also known as lipid rafts, play a crucial role in facilitating a broad range of important cell functions. Whereas on living cell membranes lipid rafts have been postulated to have nanoscopic dimensions and to be highly transient, the existence of a similar type of dynamic nanodomains in multicomponent lipid bilayers has been questioned. Here, we perform fluorescence correlation spectroscopy on planar plasmonic antenna arrays with different nanogap sizes to assess the dynamic nanoscale organization of mimetic biological membranes. Our approach takes advantage of the highly enhanced and confined excitation light provided by the nanoantennas together with their outstanding planarity to investigate membrane regions as small as 10 nm in size with microsecond time resolution. Our diffusion data are consistent with the coexistence of transient nanoscopic domains in both the liquid-ordered and the liquid-disordered microscopic phases of multicomponent lipid bilayers. These nanodomains have characteristic residence times between 30 and 150 μs and sizes around 10 nm, as inferred from the diffusion data. Thus, although microscale phase separation occurs on mimetic membranes, nanoscopic domains also coexist, suggesting that these transient assemblies might be similar to those occurring in living cells, which in the absence of raft-stabilizing proteins are poised to be short-lived. Importantly, our work underscores the high potential of photonic nanoantennas to interrogate the nanoscale heterogeneity of native biological membranes with ultrahigh spatiotemporal resolution.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-07-11
2018
2018-01-11
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/172443
https://dx.doi.org/10.1021/acsnano.7b03177
url https://hdl.handle.net/2117/172443
https://dx.doi.org/10.1021/acsnano.7b03177
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv European Commission http://doi.org/10.13039/100010661 Horizon 2020 Framework Programme 665884 ICFOstepstone PhD Programme for Early-Stage Researchers in Photonics
European Commission http://dx.doi.org/10.13039/100011102 Seventh Framework Programme 288263 Advanced photonic antenna tools for biosensing and cellular nanoimaging
European Commission http://dx.doi.org/10.13039/100011102 Seventh Framework Programme 278242 Extended fluorescence resonance energy transfer with plasmonic nanocircuits
European Commission http://doi.org/10.13039/100010661 Horizon 2020 Framework Programme 713729 ICFOstepstone PhD Programme for Early-Stage Researchers in Photonics
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ACS
publisher.none.fl_str_mv ACS
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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