Docosahexaenoic acid supplemented diet influences the orchidectomy-induced vascular dysfunction in rat mesenteric arteries

Over the past few decades, the cardiovascular benefits of a high dietary intake of long-chain polyunsaturated fatty acids (PUFAs), like docosahexaenoic acid (DHA), have been extensively studied. However, many of the molecular mechanisms and effects exerted by PUFAs have yet to be well explained. The...

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Autores: Villalpando, Diva M., Navarro Jiménez, Rocío, Campo Milán, Lara del, Largo, Carlota, Muñoz, David, Tabernero, María, Baeza, Ramiro, Otero, Cristina, García, Hugo S., Ferrer Parra, Mercedes
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/679052
Acceso en línea:http://hdl.handle.net/10486/679052
https://dx.doi.org/10.1371/journal. pone.0168841
Access Level:acceso abierto
Palabra clave:Docosahexaenoic acid
Vascular
Supplemented diet
Diet
Artery
Orchidectomy
Medicina
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spelling Docosahexaenoic acid supplemented diet influences the orchidectomy-induced vascular dysfunction in rat mesenteric arteriesVillalpando, Diva M.Navarro Jiménez, RocíoCampo Milán, Lara delLargo, CarlotaMuñoz, DavidTabernero, MaríaBaeza, RamiroOtero, CristinaGarcía, Hugo S.Ferrer Parra, MercedesDocosahexaenoic acidVascularSupplemented dietDietArteryOrchidectomyMedicinaOver the past few decades, the cardiovascular benefits of a high dietary intake of long-chain polyunsaturated fatty acids (PUFAs), like docosahexaenoic acid (DHA), have been extensively studied. However, many of the molecular mechanisms and effects exerted by PUFAs have yet to be well explained. The lack of sex hormones alters vascular tone, and we have described that a DHA-supplemented diet to orchidectomized rats improve vascular function of the aorta. Based on these data and since the mesenteric artery importantly controls the systemic vascular resistance, the objective of this study was to analyze the effect of a DHAsupplemented diet on the mesenteric vascular function from orchidectomized rats. For this purpose mesenteric artery segments obtained from control, orchidectomized or orchidectomized plus DHA-supplemented diet were utilized to analyze: (1) the release of prostanoids, (2) formation of NO and ROS, (3) the vasodilator response to acetylcholine (ACh), as well as the involvement of prostanoids and NO in this response, and (4) the vasoconstrictor response to electrical field stimulation (EFS), analyzing also the effect of exogenous noradrenaline (NA), and the NO donor, sodium nitroprusside (SNP). The results demonstrate beneficial effects of DHA on the vascular function in orchidectomized rats, which include a decrease in the prostanoids release and superoxide formation that were previously augmented by orchidectomy. Additionally, there was an increase in endothelial NO formation and the response to ACh, in which NO involvement and the participation of vasodilator prostanoids were increased. DHA also reversed the decrease in EFS-induced response caused by orchidectomy. All of these findings suggest beneficial effects of DHA on vascular function by reversing the neurogenic response and the endothelial dysfunction caused by orchidectomySupported by grants (to MF) from the Fondo de Investigaciones Sanitarias (PI1100406), Comunidad de Madrid (S2013/ABI-2783, ªINSPIRA1-CMº), Fondo Europeo de Desarrollo Regional, and Centro de Estudios América Latina (Grupo Santander-UAM)Public Library of ScienceDepartamento de FisiologíaDepartamento de MedicinaFacultad de MedicinaInstituto de Investigación Sanitaria del Hospital Universitario de La Paz (IdiPAZ)20172017-01-09research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/679052https://dx.doi.org/10.1371/journal. pone.0168841reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/6790522026-06-23T12:46:27Z
dc.title.none.fl_str_mv Docosahexaenoic acid supplemented diet influences the orchidectomy-induced vascular dysfunction in rat mesenteric arteries
title Docosahexaenoic acid supplemented diet influences the orchidectomy-induced vascular dysfunction in rat mesenteric arteries
spellingShingle Docosahexaenoic acid supplemented diet influences the orchidectomy-induced vascular dysfunction in rat mesenteric arteries
Villalpando, Diva M.
Docosahexaenoic acid
Vascular
Supplemented diet
Diet
Artery
Orchidectomy
Medicina
title_short Docosahexaenoic acid supplemented diet influences the orchidectomy-induced vascular dysfunction in rat mesenteric arteries
title_full Docosahexaenoic acid supplemented diet influences the orchidectomy-induced vascular dysfunction in rat mesenteric arteries
title_fullStr Docosahexaenoic acid supplemented diet influences the orchidectomy-induced vascular dysfunction in rat mesenteric arteries
title_full_unstemmed Docosahexaenoic acid supplemented diet influences the orchidectomy-induced vascular dysfunction in rat mesenteric arteries
title_sort Docosahexaenoic acid supplemented diet influences the orchidectomy-induced vascular dysfunction in rat mesenteric arteries
dc.creator.none.fl_str_mv Villalpando, Diva M.
Navarro Jiménez, Rocío
Campo Milán, Lara del
Largo, Carlota
Muñoz, David
Tabernero, María
Baeza, Ramiro
Otero, Cristina
García, Hugo S.
Ferrer Parra, Mercedes
author Villalpando, Diva M.
author_facet Villalpando, Diva M.
Navarro Jiménez, Rocío
Campo Milán, Lara del
Largo, Carlota
Muñoz, David
Tabernero, María
Baeza, Ramiro
Otero, Cristina
García, Hugo S.
Ferrer Parra, Mercedes
author_role author
author2 Navarro Jiménez, Rocío
Campo Milán, Lara del
Largo, Carlota
Muñoz, David
Tabernero, María
Baeza, Ramiro
Otero, Cristina
García, Hugo S.
Ferrer Parra, Mercedes
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Fisiología
Departamento de Medicina
Facultad de Medicina
Instituto de Investigación Sanitaria del Hospital Universitario de La Paz (IdiPAZ)
dc.subject.none.fl_str_mv Docosahexaenoic acid
Vascular
Supplemented diet
Diet
Artery
Orchidectomy
Medicina
topic Docosahexaenoic acid
Vascular
Supplemented diet
Diet
Artery
Orchidectomy
Medicina
description Over the past few decades, the cardiovascular benefits of a high dietary intake of long-chain polyunsaturated fatty acids (PUFAs), like docosahexaenoic acid (DHA), have been extensively studied. However, many of the molecular mechanisms and effects exerted by PUFAs have yet to be well explained. The lack of sex hormones alters vascular tone, and we have described that a DHA-supplemented diet to orchidectomized rats improve vascular function of the aorta. Based on these data and since the mesenteric artery importantly controls the systemic vascular resistance, the objective of this study was to analyze the effect of a DHAsupplemented diet on the mesenteric vascular function from orchidectomized rats. For this purpose mesenteric artery segments obtained from control, orchidectomized or orchidectomized plus DHA-supplemented diet were utilized to analyze: (1) the release of prostanoids, (2) formation of NO and ROS, (3) the vasodilator response to acetylcholine (ACh), as well as the involvement of prostanoids and NO in this response, and (4) the vasoconstrictor response to electrical field stimulation (EFS), analyzing also the effect of exogenous noradrenaline (NA), and the NO donor, sodium nitroprusside (SNP). The results demonstrate beneficial effects of DHA on the vascular function in orchidectomized rats, which include a decrease in the prostanoids release and superoxide formation that were previously augmented by orchidectomy. Additionally, there was an increase in endothelial NO formation and the response to ACh, in which NO involvement and the participation of vasodilator prostanoids were increased. DHA also reversed the decrease in EFS-induced response caused by orchidectomy. All of these findings suggest beneficial effects of DHA on vascular function by reversing the neurogenic response and the endothelial dysfunction caused by orchidectomy
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-09
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/679052
https://dx.doi.org/10.1371/journal. pone.0168841
url http://hdl.handle.net/10486/679052
https://dx.doi.org/10.1371/journal. pone.0168841
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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