Clinical and diagnostic aspects of feline cutaneous leishmaniosis in Venezuela
Abstract Background: Venezuela is an endemic area for human and canine leishmaniosis due to Leishmania infantum and parasites of the Leishmania braziliensis and L. mexicana complexes. Limited data are available on feline leishmaniosis (FeL) in this region. The aim of this study was to describe clini...
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2018 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2445/122563 |
| Acceso en línea: | https://hdl.handle.net/2445/122563 |
| Access Level: | acceso abierto |
| Palabra clave: | Leishmaniosi Gats Immunohistoquímica Veneçuela Leishmaniasis Cats Immunohistochemistry Venezuela |
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Clinical and diagnostic aspects of feline cutaneous leishmaniosis in VenezuelaKalú Rivas, AruaniAlcover Amengual, Maria MagdalenaMartínez-Orellana, PamelaMontserrat-Sangrà, SaraNachum-Biala, YaaritBardagí, MarFisa Saladrigas, RoserRiera Lizandra, Ma. CristinaBaneth, GadSolano-Gallego, LaiaLeishmaniosiGatsImmunohistoquímicaVeneçuelaLeishmaniasisCatsImmunohistochemistryVenezuelaAbstract Background: Venezuela is an endemic area for human and canine leishmaniosis due to Leishmania infantum and parasites of the Leishmania braziliensis and L. mexicana complexes. Limited data are available on feline leishmaniosis (FeL) in this region. The aim of this study was to describe clinical and diagnostic aspects of FeL in Venezuela. Results: Thirty-one domestic cats from urban areas of Lara State in Venezuela were enrolled. Twenty-five were healthy. Six other cats had solitary or multiple nodular lesions, which were located on the nose; ears; ears and nose; and nose, ears, tail and lower limbs. Cutaneous lesions were characterized by diffuse pyogranulomatous infiltrate in all sick cats with numerous intracellular and extracellular amastigotes, and immunohistochemistry was positive for Leishmania in five sick cats. All healthy cats were seronegative for L. infantum and L. braziliensis antigens by ELISA. Two out of five sick cats yielded a positive ELISA result to both Leishmania antigens with higher antibody levels to L. braziliensis compared to L. infantum. Significantly higher antibody levels by ELISA as well as a higher number of bands by Western blot (WB) were found for L. braziliensis when compared to L. infantum antigens in all sera from Venezuelan sick and healthy cats. All healthy cats were blood Leishmania spp. qPCR negative while three out of six sick cats were blood qPCR positive. All paraffin-embedded skin biopsies (n = 4) as well as cutaneous cytology (n = 3) were positive by Leishmania spp. qPCR in sick cats. Leishmania speciation was obtained only from the cutaneous lesion samples from cytological preparations of two out of three sick cats which were identified as infected with L. mexicana or a closely related specie. Conclusions: FeL should be included in the differential diagnosis list of nodular-ulcerative lesions. The most reliable diagnostic technique in sick cats is cytological or histopathological examination along with immunohistochemistry, since blood PCR and serology by ELISA might be negative. WB appears to be more sensitive in detecting infection. Cats with leishmaniosis from Venezuela are most likely infected with species of L. mexicana or a closely related species or the L. braziliensis species complex and not with L. infantum.BioMed Central2018201820182018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/2445/122563Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1186/s13071-018-2747-2Parasites & Vectors, 2018, vol. 11, num. 1, p. 141https://doi.org/10.1186/s13071-018-2747-2cc-by (c) Kalú Rivas, Aruani et al., 2018http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1225632026-05-29T05:05:01Z |
| dc.title.none.fl_str_mv |
Clinical and diagnostic aspects of feline cutaneous leishmaniosis in Venezuela |
| title |
Clinical and diagnostic aspects of feline cutaneous leishmaniosis in Venezuela |
| spellingShingle |
Clinical and diagnostic aspects of feline cutaneous leishmaniosis in Venezuela Kalú Rivas, Aruani Leishmaniosi Gats Immunohistoquímica Veneçuela Leishmaniasis Cats Immunohistochemistry Venezuela |
| title_short |
Clinical and diagnostic aspects of feline cutaneous leishmaniosis in Venezuela |
| title_full |
Clinical and diagnostic aspects of feline cutaneous leishmaniosis in Venezuela |
| title_fullStr |
Clinical and diagnostic aspects of feline cutaneous leishmaniosis in Venezuela |
| title_full_unstemmed |
Clinical and diagnostic aspects of feline cutaneous leishmaniosis in Venezuela |
| title_sort |
Clinical and diagnostic aspects of feline cutaneous leishmaniosis in Venezuela |
| dc.creator.none.fl_str_mv |
Kalú Rivas, Aruani Alcover Amengual, Maria Magdalena Martínez-Orellana, Pamela Montserrat-Sangrà, Sara Nachum-Biala, Yaarit Bardagí, Mar Fisa Saladrigas, Roser Riera Lizandra, Ma. Cristina Baneth, Gad Solano-Gallego, Laia |
| author |
Kalú Rivas, Aruani |
| author_facet |
Kalú Rivas, Aruani Alcover Amengual, Maria Magdalena Martínez-Orellana, Pamela Montserrat-Sangrà, Sara Nachum-Biala, Yaarit Bardagí, Mar Fisa Saladrigas, Roser Riera Lizandra, Ma. Cristina Baneth, Gad Solano-Gallego, Laia |
| author_role |
author |
| author2 |
Alcover Amengual, Maria Magdalena Martínez-Orellana, Pamela Montserrat-Sangrà, Sara Nachum-Biala, Yaarit Bardagí, Mar Fisa Saladrigas, Roser Riera Lizandra, Ma. Cristina Baneth, Gad Solano-Gallego, Laia |
| author2_role |
author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Leishmaniosi Gats Immunohistoquímica Veneçuela Leishmaniasis Cats Immunohistochemistry Venezuela |
| topic |
Leishmaniosi Gats Immunohistoquímica Veneçuela Leishmaniasis Cats Immunohistochemistry Venezuela |
| description |
Abstract Background: Venezuela is an endemic area for human and canine leishmaniosis due to Leishmania infantum and parasites of the Leishmania braziliensis and L. mexicana complexes. Limited data are available on feline leishmaniosis (FeL) in this region. The aim of this study was to describe clinical and diagnostic aspects of FeL in Venezuela. Results: Thirty-one domestic cats from urban areas of Lara State in Venezuela were enrolled. Twenty-five were healthy. Six other cats had solitary or multiple nodular lesions, which were located on the nose; ears; ears and nose; and nose, ears, tail and lower limbs. Cutaneous lesions were characterized by diffuse pyogranulomatous infiltrate in all sick cats with numerous intracellular and extracellular amastigotes, and immunohistochemistry was positive for Leishmania in five sick cats. All healthy cats were seronegative for L. infantum and L. braziliensis antigens by ELISA. Two out of five sick cats yielded a positive ELISA result to both Leishmania antigens with higher antibody levels to L. braziliensis compared to L. infantum. Significantly higher antibody levels by ELISA as well as a higher number of bands by Western blot (WB) were found for L. braziliensis when compared to L. infantum antigens in all sera from Venezuelan sick and healthy cats. All healthy cats were blood Leishmania spp. qPCR negative while three out of six sick cats were blood qPCR positive. All paraffin-embedded skin biopsies (n = 4) as well as cutaneous cytology (n = 3) were positive by Leishmania spp. qPCR in sick cats. Leishmania speciation was obtained only from the cutaneous lesion samples from cytological preparations of two out of three sick cats which were identified as infected with L. mexicana or a closely related specie. Conclusions: FeL should be included in the differential diagnosis list of nodular-ulcerative lesions. The most reliable diagnostic technique in sick cats is cytological or histopathological examination along with immunohistochemistry, since blood PCR and serology by ELISA might be negative. WB appears to be more sensitive in detecting infection. Cats with leishmaniosis from Venezuela are most likely infected with species of L. mexicana or a closely related species or the L. braziliensis species complex and not with L. infantum. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018 2018 2018 2018 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2445/122563 |
| url |
https://hdl.handle.net/2445/122563 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Reproducció del document publicat a: https://doi.org/10.1186/s13071-018-2747-2 Parasites & Vectors, 2018, vol. 11, num. 1, p. 141 https://doi.org/10.1186/s13071-018-2747-2 |
| dc.rights.none.fl_str_mv |
cc-by (c) Kalú Rivas, Aruani et al., 2018 http://creativecommons.org/licenses/by/3.0/es info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
cc-by (c) Kalú Rivas, Aruani et al., 2018 http://creativecommons.org/licenses/by/3.0/es |
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openAccess |
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application/pdf application/pdf |
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BioMed Central |
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BioMed Central |
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Articles publicats en revistes (Biologia, Sanitat i Medi Ambient) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Recercat. Dipósit de la Recerca de Catalunya |
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