Antifungal and anti-virulence activities of cinnamon, thyme, and clove essential oils against Candida species

BackgroundCandida species are major opportunistic pathogens, with Candida albicans being the most frequent cause of candidiasis. However, increasing rates of non-albicans infections and antifungal resistance bring an urgent need for new therapeutics. Essential oils (EOs) have gained attention due to...

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Detalles Bibliográficos
Autores: Ahaik, Islam, Nuñez Rodriguez, Juan Carlos, Abelló Cros, Sònia, Yanes, Oscar, Bouhdid, Samira, Gabaldón, Toni
Tipo de recurso: artículo
Fecha de publicación:2026
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:dnet:upcommonspor::9f38220f8215a9ed308ab74160b52229
Acceso en línea:https://hdl.handle.net/2117/460928
https://dx.doi.org/10.3389/fphar.2026.1756267
Access Level:acceso abierto
Palabra clave:Antifungal resistance
Candida
Essential oils
Non-albicans Candida
Virulence factors
Àrees temàtiques de la UPC::Informàtica::Aplicacions de la informàtica::Bioinformàtica
Descripción
Sumario:BackgroundCandida species are major opportunistic pathogens, with Candida albicans being the most frequent cause of candidiasis. However, increasing rates of non-albicans infections and antifungal resistance bring an urgent need for new therapeutics. Essential oils (EOs) have gained attention due to their potential to inhibit fungal growth and virulence.MethodsThe chemical composition of cinnamon, thyme and clove EOs was analyzed by gas chromatography-mass spectrometry (GC-MS). Antifungal activity was evaluated against eighteen Candida strains representing nine species, including multidrug-resistant isolates. Minimum inhibitory concentrations (MICs) were determined. The two most active EOs were further assessed for their effects on germ tube formation and protease production, two key virulence traits.ResultsGC-MS identified cinnamaldehyde, thymol and eugenol as the dominant components of cinnamon, thyme and clove EOs, respectively (> 70% relative abundance). All EOs displayed antifungal activity, with cinnamon and thyme being the most potent. Both oils showed increased activity against multidrug-resistant strains of Candida parapsilosis and Nakaseomyces glabratus compared with their susceptible parentals, suggesting they may target resistance trade-offs. All five clades of Candidozyma auris displayed low MICs for cinnamon EO (0.002-0.008% v/v), indicating high susceptibility. Cinnamon EO reduced germ tube formation in C. albicans from 97% to 12% at MIC/2, while thyme EO completely inhibited germ tube formation and induced pseudohyphae. Protease production was totally suppressed in C. auris clades II and III at MIC/2 thyme EO.ConclusionThese results highlight the strong dual activity of EOs, supporting further exploration of their potential as complementary therapeutic options against Candida infections.