Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci Species

Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).

Detalhes bibliográficos
Autores: Silva, Vanessa, Correira, Elisete, Pereira, José Eduardo, González Machado, Camino, Capita González, Rosa María, Alonso Calleja, Carlos, Igrejas, Gilberto, Poeta, Patrícia
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Recursos:Universidad Rey Juan Carlos
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/20244
Acesso em linha:https://www.mdpi.com/2076-0817/11/6/689
https://hdl.handle.net/10612/20244
Access Level:acceso abierto
Palavra-chave:Tecnología de los alimentos
Biofilm
Staphylococci
Coagulase-negative staphylococci (CoNS)
2414.04 Bacteriología
3309 Tecnología de Los Alimentos
3109.05 Microbiología
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spelling Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci SpeciesSilva, VanessaCorreira, ElisetePereira, José EduardoGonzález Machado, CaminoCapita González, Rosa MaríaAlonso Calleja, CarlosIgrejas, GilbertoPoeta, PatríciaTecnología de los alimentosBiofilmStaphylococciCoagulase-negative staphylococci (CoNS)2414.04 Bacteriología3309 Tecnología de Los Alimentos3109.05 MicrobiologíaCopyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).[EN] The ability of biofilm formation seems to play an important role in the virulence of staphylococci. However, studies reporting biofilm formation of coagulase-negative staphylococci isolated from animals are still very scarce. Thus, we aimed to evaluate the biofilm-forming capacity of CoNS and S. pseudintermedius isolated from several animal species and to investigate the effect of conventional antimicrobials on biofilm reduction. A total of 35 S. pseudintermedius and 192 CoNS were included. Biofilm formation was accessed by the microtiter plate assay and the biofilms were stained by crystal violet. Association between biofilm formation and staphylococci species and antimicrobial resistance was also performed. Biofilm susceptibility testing was performed with tetracycline and amikacin at the minimum inhibitory concentration (MIC) and 10 × MIC. The metabolic activity of the biofilm cells after antimicrobial treatment was accessed by the XTT assay. All isolates formed biofilm, with S. urealyticus producing the most biofilm biomass and S. pseudintermedius producing the least biomass. There was a positive association between biofilm formation and multidrug resistance as well as resistance to individual antimicrobials. Neither tetracycline nor amikacin were able to eradicate the biofilm, not even at the highest concentration used. This study provides new insights into biofilm formation and the effects of antimicrobials on CoNS species.SIThis work was funded by the R&D Project CAREBIO2: Comparative assessment of antimicrobial resistance in environmental biofilms through proteomics—towards innovative theranostic biomarkers, with reference NORTE-01-0145-FEDER-030101 and PTDC/SAU-INF/30101/2017, financed by the European Regional Development Fund (ERDF) through the Northern Regional Operational Program (NORTE 2020) and the Foundation for Science and Technology (FCT). This work was supported by the Associate Laboratory for Green Chemistry-LAQV, which is financed by national funds from FCT/MCTES (UIDB/50006/2020 and UIDP/50006/2020) and by the projects UIDB/CVT/00772/2020 and LA/P/0059/2020 funded by the Portuguese Foundation for Science and Technology (FCT). The Ministerio de Ciencia, Innovación y Universidades (Spain, grant number RTI2018-098267-R-C33) and the Junta de Castilla y León (Consejería de Educación, Spain, grant number LE018P20). Vanessa Silva is grateful to FCT (Fundacão para a Ciência e a Tecnologia) for financial support through the PhD grant SFRH/BD/137947/2018.MDPINutricion y BromatologiaFacultad de Veterinaria2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://www.mdpi.com/2076-0817/11/6/689https://hdl.handle.net/10612/20244reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad Rey Juan CarlosInglésinfo:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/RTI2018-098267-R-C33http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/202442026-06-24T12:43:27Z
dc.title.none.fl_str_mv Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci Species
title Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci Species
spellingShingle Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci Species
Silva, Vanessa
Tecnología de los alimentos
Biofilm
Staphylococci
Coagulase-negative staphylococci (CoNS)
2414.04 Bacteriología
3309 Tecnología de Los Alimentos
3109.05 Microbiología
title_short Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci Species
title_full Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci Species
title_fullStr Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci Species
title_full_unstemmed Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci Species
title_sort Exploring the Biofilm Formation Capacity in S. pseudintermedius and Coagulase-Negative Staphylococci Species
dc.creator.none.fl_str_mv Silva, Vanessa
Correira, Elisete
Pereira, José Eduardo
González Machado, Camino
Capita González, Rosa María
Alonso Calleja, Carlos
Igrejas, Gilberto
Poeta, Patrícia
author Silva, Vanessa
author_facet Silva, Vanessa
Correira, Elisete
Pereira, José Eduardo
González Machado, Camino
Capita González, Rosa María
Alonso Calleja, Carlos
Igrejas, Gilberto
Poeta, Patrícia
author_role author
author2 Correira, Elisete
Pereira, José Eduardo
González Machado, Camino
Capita González, Rosa María
Alonso Calleja, Carlos
Igrejas, Gilberto
Poeta, Patrícia
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Nutricion y Bromatologia
Facultad de Veterinaria
dc.subject.none.fl_str_mv Tecnología de los alimentos
Biofilm
Staphylococci
Coagulase-negative staphylococci (CoNS)
2414.04 Bacteriología
3309 Tecnología de Los Alimentos
3109.05 Microbiología
topic Tecnología de los alimentos
Biofilm
Staphylococci
Coagulase-negative staphylococci (CoNS)
2414.04 Bacteriología
3309 Tecnología de Los Alimentos
3109.05 Microbiología
description Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.mdpi.com/2076-0817/11/6/689
https://hdl.handle.net/10612/20244
url https://www.mdpi.com/2076-0817/11/6/689
https://hdl.handle.net/10612/20244
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/RTI2018-098267-R-C33
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad Rey Juan Carlos
instname_str Universidad Rey Juan Carlos
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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