Development of displacement electrochemical inmunosensors: the case of 2,4,6-trichloroanisole
The purpose of this work is to explore and exploit the principles of Displacement Electrochemical Immunosensing (DEI) and Indirect Competitive ELISA (ICE) to detect 2,4,6-trichloroanisole (TCA). The rational design of indirect competitive ELISA for TCA detection is attempted. The developed assay det...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2007 |
| País: | España |
| Institución: | Universitat Rovira i virgili (URV) |
| Repositorio: | Repositori Institucional de la Universitat Rovira i Virgili |
| OAI Identifier: | oai:urv.cat:TDX:336 |
| Acceso en línea: | https://hdl.handle.net/20.500.11797/TDX336 http://hdl.handle.net/10803/8554 |
| Access Level: | acceso abierto |
| Palabra clave: | 62 - Enginyeria. Tecnologia |
| Sumario: | The purpose of this work is to explore and exploit the principles of Displacement Electrochemical Immunosensing (DEI) and Indirect Competitive ELISA (ICE) to detect 2,4,6-trichloroanisole (TCA). The rational design of indirect competitive ELISA for TCA detection is attempted. The developed assay detects TCA at concentrations from 1ppt to 1 ppm, with a limit of detection of 4.2 ppt. The assay can be commercially useful in situations where less than 80 minutes total assay time is required. A mathematical model (MM) is developed for the rational design of an electrochemical displacement immunosensor (DEI). Despite the low affinity constants of the antibodies obtained for this work a functional DEI is developed with the predicted by the MM high limit of detection for TCA (0.2 ppm). The non-specific adsorption (NSA) of proteins is identified as a critical problem inhibiting further optimization of the DEI. The use non-insulating Cu UPD as NSA controller or electrochemically compatible blocking, together with amperometric displacement detection are proposed as a platform that could permit further development of reagentless and labelless immunosensors. |
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