Generation of mesenchymal stromal cells from urine-derived iPSCs of pediatric brain tumor patients
Human induced pluripotent stem cells (iPSCs) provide a virtually inexhaustible source of starting material for next generation cell therapies, offering new opportunities for regenerative medicine. Among different cell sources for the generation of iPSCs, urine cells are clinically relevant since the...
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Universidad de Sevilla (US) |
| Repositorio: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:idus.us.es:11441/173358 |
| Acceso en línea: | https://hdl.handle.net/11441/173358 https://doi.org/10.3389/fimmu.2023.1022676 |
| Access Level: | acceso abierto |
| Palabra clave: | iPSC mesenchymal stem/stromal cells (MSC) central nervous system cancer children cell therapy cell reprogramming oncology cancer |
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Generation of mesenchymal stromal cells from urine-derived iPSCs of pediatric brain tumor patientsBaliña Sánchez, CarmenAguilera, YolandaAdán, NormaSierra Párraga, Jesús MaríaOlmedo Moreno, LauraPanadero Morón, ConcepciónCabello Laureano, RosaMárquez Vega, CatalinaMartín Montalvo, AlejandroCapilla González, VivianiPSCmesenchymal stem/stromal cells (MSC)central nervous system cancerchildrencell therapycell reprogrammingoncologycancerHuman induced pluripotent stem cells (iPSCs) provide a virtually inexhaustible source of starting material for next generation cell therapies, offering new opportunities for regenerative medicine. Among different cell sources for the generation of iPSCs, urine cells are clinically relevant since these cells can be repeatedly obtained by non-invasive methods from patients of any age and health condition. These attributes encourage patients to participate in preclinical and clinical research. In particular, the use of urine-derived iPSC products is a convenient strategy for children with brain tumors, which are medically fragile patients. Here, we investigate the feasibility of using urine samples as a source of somatic cells to generate iPSC lines from pediatric patients with brain tumors (BT-iPSC). Urinary epithelial cells were isolated and reprogrammed using non-integrative Sendai virus vectors harboring the Yamanaka factors KLF4, OCT3/4, SOX2 and C-MYC. After reprogramming, BT-iPSC lines were subject to quality assessment and were compared to iPSCs obtained from urine samples of non-tumor pediatric patients (nonT-iPSC). We demonstrated that iPSCs can be successfully derived from a small volume of urine obtained from pediatric patients. Importantly, we showed that BT-iPSCs are equivalent to nonT-iPSCs in terms of morphology, pluripotency, and differentiation capacity into the three germ layers. In addition, both BT-iPSCs and nonT-iPSCs efficiently differentiated into functional mesenchymal stem/stromal cells (iMSC) with immunomodulatory properties. Therefore, this study provides an attractive approach to non-invasively generate personalized iMSC products intended for the treatment of children with brain tumors.Frontiers MediaFarmacología, Pediatría y RadiologíaInstituto de Salud Carlos IIIAsociación Española Contra el Cáncer (AECC)Junta de AndalucíaEuropean Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)Gobierno de España2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/173358https://doi.org/10.3389/fimmu.2023.1022676reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésFrontiers in Immunology, 14, 1022676.CP19/00046PI20/00341IDEAS20051CAPIPY20/00481PY20/00480PID2021-123965OB-I00FPU19/04703POSTDOC/21/00424https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.1022676/fullinfo:eu-repo/semantics/openAccessoai:idus.us.es:11441/1733582026-06-17T12:51:07Z |
| dc.title.none.fl_str_mv |
Generation of mesenchymal stromal cells from urine-derived iPSCs of pediatric brain tumor patients |
| title |
Generation of mesenchymal stromal cells from urine-derived iPSCs of pediatric brain tumor patients |
| spellingShingle |
Generation of mesenchymal stromal cells from urine-derived iPSCs of pediatric brain tumor patients Baliña Sánchez, Carmen iPSC mesenchymal stem/stromal cells (MSC) central nervous system cancer children cell therapy cell reprogramming oncology cancer |
| title_short |
Generation of mesenchymal stromal cells from urine-derived iPSCs of pediatric brain tumor patients |
| title_full |
Generation of mesenchymal stromal cells from urine-derived iPSCs of pediatric brain tumor patients |
| title_fullStr |
Generation of mesenchymal stromal cells from urine-derived iPSCs of pediatric brain tumor patients |
| title_full_unstemmed |
Generation of mesenchymal stromal cells from urine-derived iPSCs of pediatric brain tumor patients |
| title_sort |
Generation of mesenchymal stromal cells from urine-derived iPSCs of pediatric brain tumor patients |
| dc.creator.none.fl_str_mv |
Baliña Sánchez, Carmen Aguilera, Yolanda Adán, Norma Sierra Párraga, Jesús María Olmedo Moreno, Laura Panadero Morón, Concepción Cabello Laureano, Rosa Márquez Vega, Catalina Martín Montalvo, Alejandro Capilla González, Vivian |
| author |
Baliña Sánchez, Carmen |
| author_facet |
Baliña Sánchez, Carmen Aguilera, Yolanda Adán, Norma Sierra Párraga, Jesús María Olmedo Moreno, Laura Panadero Morón, Concepción Cabello Laureano, Rosa Márquez Vega, Catalina Martín Montalvo, Alejandro Capilla González, Vivian |
| author_role |
author |
| author2 |
Aguilera, Yolanda Adán, Norma Sierra Párraga, Jesús María Olmedo Moreno, Laura Panadero Morón, Concepción Cabello Laureano, Rosa Márquez Vega, Catalina Martín Montalvo, Alejandro Capilla González, Vivian |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Farmacología, Pediatría y Radiología Instituto de Salud Carlos III Asociación Española Contra el Cáncer (AECC) Junta de Andalucía European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) Gobierno de España |
| dc.subject.none.fl_str_mv |
iPSC mesenchymal stem/stromal cells (MSC) central nervous system cancer children cell therapy cell reprogramming oncology cancer |
| topic |
iPSC mesenchymal stem/stromal cells (MSC) central nervous system cancer children cell therapy cell reprogramming oncology cancer |
| description |
Human induced pluripotent stem cells (iPSCs) provide a virtually inexhaustible source of starting material for next generation cell therapies, offering new opportunities for regenerative medicine. Among different cell sources for the generation of iPSCs, urine cells are clinically relevant since these cells can be repeatedly obtained by non-invasive methods from patients of any age and health condition. These attributes encourage patients to participate in preclinical and clinical research. In particular, the use of urine-derived iPSC products is a convenient strategy for children with brain tumors, which are medically fragile patients. Here, we investigate the feasibility of using urine samples as a source of somatic cells to generate iPSC lines from pediatric patients with brain tumors (BT-iPSC). Urinary epithelial cells were isolated and reprogrammed using non-integrative Sendai virus vectors harboring the Yamanaka factors KLF4, OCT3/4, SOX2 and C-MYC. After reprogramming, BT-iPSC lines were subject to quality assessment and were compared to iPSCs obtained from urine samples of non-tumor pediatric patients (nonT-iPSC). We demonstrated that iPSCs can be successfully derived from a small volume of urine obtained from pediatric patients. Importantly, we showed that BT-iPSCs are equivalent to nonT-iPSCs in terms of morphology, pluripotency, and differentiation capacity into the three germ layers. In addition, both BT-iPSCs and nonT-iPSCs efficiently differentiated into functional mesenchymal stem/stromal cells (iMSC) with immunomodulatory properties. Therefore, this study provides an attractive approach to non-invasively generate personalized iMSC products intended for the treatment of children with brain tumors. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/11441/173358 https://doi.org/10.3389/fimmu.2023.1022676 |
| url |
https://hdl.handle.net/11441/173358 https://doi.org/10.3389/fimmu.2023.1022676 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Frontiers in Immunology, 14, 1022676. CP19/00046 PI20/00341 IDEAS20051CAPI PY20/00481 PY20/00480 PID2021-123965OB-I00 FPU19/04703 POSTDOC/21/00424 https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.1022676/full |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf application/pdf |
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Frontiers Media |
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Frontiers Media |
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reponame:idUS. Depósito de Investigación de la Universidad de Sevilla instname:Universidad de Sevilla (US) |
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Universidad de Sevilla (US) |
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idUS. Depósito de Investigación de la Universidad de Sevilla |
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