Metallo-Liposomes Derived from the [Ru(bpy)3]2+ Complex as Nanocarriers of Therapeutic Agents

The obtaining of nanocarriers of gene material and small drugs is still an interesting research line. Side-effects produced by the toxicity of several pharmaceutics, the high concentrations needed to get therapeutic effects, or their excessive use by patients have motivated the search for new nanost...

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Autores: Moyá Morán, María Luisa, Ostos Marcos, Francisco José, Moreno, Izamar, García, Diandra, Moreno Gordillo, Paula, Valle Rosado, Iván, López-Cornejo, María del Pilar, Lebrón Romero, José Antonio, López López, Manuel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/115174
Acceso en línea:https://hdl.handle.net/11441/115174
https://doi.org/10.3390/chemosensors9050090
Access Level:acceso abierto
Palabra clave:Metallo-liposome
Nanocarrier
Gene therapy
DNA
Doxorubicin
TEM
Sensor
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spelling Metallo-Liposomes Derived from the [Ru(bpy)3]2+ Complex as Nanocarriers of Therapeutic AgentsMoyá Morán, María LuisaOstos Marcos, Francisco JoséMoreno, IzamarGarcía, DiandraMoreno Gordillo, PaulaValle Rosado, IvánLópez-Cornejo, María del PilarLebrón Romero, José AntonioLópez López, ManuelMetallo-liposomeNanocarrierGene therapyDNADoxorubicinTEMSensorThe obtaining of nanocarriers of gene material and small drugs is still an interesting research line. Side-effects produced by the toxicity of several pharmaceutics, the high concentrations needed to get therapeutic effects, or their excessive use by patients have motivated the search for new nanostructures. For these reasons, cationic metallo-liposomes composed by phosphatidylcholine (PC), cholesterol (CHO) and RuC1C19 (a surfactant derived from the metallic complex [Ru(bpy)3]2+) were prepared and characterized by using diverse techniques (zeta potential, dynamic light scattering and electronic transmission microscopy –TEM-). Unimodal or bimodal populations of spherical aggregates with small sizes were obtained depending on the composition of the liposomes. The presence of cholesterol favored the formation of small aggregates. ct-DNA was condensed in the presence of the liposomes investigated. In-vitro assays demonstrated the ability of these nanoaggregates to internalize into different cell lines. A positive gene transfection into human bone osteosarcoma epithelial cells (U2OS) was also observed. The RuC1C19 surfactant was used as sensor to quantify the binding of DNA to the liposomes. Doxorubicin was encapsulated into the metallo-liposomes, demonstrating their ability to be also used as nanocarriers of drugs. A relationship between then encapsulation percentage of the antibiotic and the composition of the aggregates has been established.Junta de Andalucía 2019/FQM-206, 2019/FQM-274Ministerio de Ciencia e Innovación RTI2018-100692-BI00, PI-0005-2018, P18-RT-1271Multidisciplinary Digital Publishing InstituteQuímica Física2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/115174https://doi.org/10.3390/chemosensors9050090reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésChemosensors, 9 (5), 90.2019/FQM-2062019/FQM-274RTI2018-100692-BI00PI-0005-2018P18-RT-1271https://doi.org/10.3390/chemosensors9050090info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1151742026-06-17T12:51:07Z
dc.title.none.fl_str_mv Metallo-Liposomes Derived from the [Ru(bpy)3]2+ Complex as Nanocarriers of Therapeutic Agents
title Metallo-Liposomes Derived from the [Ru(bpy)3]2+ Complex as Nanocarriers of Therapeutic Agents
spellingShingle Metallo-Liposomes Derived from the [Ru(bpy)3]2+ Complex as Nanocarriers of Therapeutic Agents
Moyá Morán, María Luisa
Metallo-liposome
Nanocarrier
Gene therapy
DNA
Doxorubicin
TEM
Sensor
title_short Metallo-Liposomes Derived from the [Ru(bpy)3]2+ Complex as Nanocarriers of Therapeutic Agents
title_full Metallo-Liposomes Derived from the [Ru(bpy)3]2+ Complex as Nanocarriers of Therapeutic Agents
title_fullStr Metallo-Liposomes Derived from the [Ru(bpy)3]2+ Complex as Nanocarriers of Therapeutic Agents
title_full_unstemmed Metallo-Liposomes Derived from the [Ru(bpy)3]2+ Complex as Nanocarriers of Therapeutic Agents
title_sort Metallo-Liposomes Derived from the [Ru(bpy)3]2+ Complex as Nanocarriers of Therapeutic Agents
dc.creator.none.fl_str_mv Moyá Morán, María Luisa
Ostos Marcos, Francisco José
Moreno, Izamar
García, Diandra
Moreno Gordillo, Paula
Valle Rosado, Iván
López-Cornejo, María del Pilar
Lebrón Romero, José Antonio
López López, Manuel
author Moyá Morán, María Luisa
author_facet Moyá Morán, María Luisa
Ostos Marcos, Francisco José
Moreno, Izamar
García, Diandra
Moreno Gordillo, Paula
Valle Rosado, Iván
López-Cornejo, María del Pilar
Lebrón Romero, José Antonio
López López, Manuel
author_role author
author2 Ostos Marcos, Francisco José
Moreno, Izamar
García, Diandra
Moreno Gordillo, Paula
Valle Rosado, Iván
López-Cornejo, María del Pilar
Lebrón Romero, José Antonio
López López, Manuel
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Química Física
dc.subject.none.fl_str_mv Metallo-liposome
Nanocarrier
Gene therapy
DNA
Doxorubicin
TEM
Sensor
topic Metallo-liposome
Nanocarrier
Gene therapy
DNA
Doxorubicin
TEM
Sensor
description The obtaining of nanocarriers of gene material and small drugs is still an interesting research line. Side-effects produced by the toxicity of several pharmaceutics, the high concentrations needed to get therapeutic effects, or their excessive use by patients have motivated the search for new nanostructures. For these reasons, cationic metallo-liposomes composed by phosphatidylcholine (PC), cholesterol (CHO) and RuC1C19 (a surfactant derived from the metallic complex [Ru(bpy)3]2+) were prepared and characterized by using diverse techniques (zeta potential, dynamic light scattering and electronic transmission microscopy –TEM-). Unimodal or bimodal populations of spherical aggregates with small sizes were obtained depending on the composition of the liposomes. The presence of cholesterol favored the formation of small aggregates. ct-DNA was condensed in the presence of the liposomes investigated. In-vitro assays demonstrated the ability of these nanoaggregates to internalize into different cell lines. A positive gene transfection into human bone osteosarcoma epithelial cells (U2OS) was also observed. The RuC1C19 surfactant was used as sensor to quantify the binding of DNA to the liposomes. Doxorubicin was encapsulated into the metallo-liposomes, demonstrating their ability to be also used as nanocarriers of drugs. A relationship between then encapsulation percentage of the antibiotic and the composition of the aggregates has been established.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/115174
https://doi.org/10.3390/chemosensors9050090
url https://hdl.handle.net/11441/115174
https://doi.org/10.3390/chemosensors9050090
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Chemosensors, 9 (5), 90.
2019/FQM-206
2019/FQM-274
RTI2018-100692-BI00
PI-0005-2018
P18-RT-1271
https://doi.org/10.3390/chemosensors9050090
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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