Evaluation of Sweat-Sampling Procedures for Human Stress-Biomarker Detection

Sweat is a potential biological fluid for the non-invasive analytical assessment of diverse molecules, including biomarkers. Notwithstanding, the sampling methodology is critical, and it must be assessed prior to using sweat for clinical diagnosis. In the current work, the analytical methodology was...

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Autores: Nunes, Maria João, Moura, José J.G., Noronha, João Paulo, Branco, Luís Cobra, Samhan Arias, Alejandro Khalil, Sousa, João P., Rouco, Carlos, Cordas, Cristina M.
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/713656
Acceso en línea:http://hdl.handle.net/10486/713656
https://dx.doi.org/10.3390/analytica3020013
Access Level:acceso abierto
Palabra clave:biomarkers
blood analysis
LC-MS/MS
sampling
stress
sweat
Medicina
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spelling Evaluation of Sweat-Sampling Procedures for Human Stress-Biomarker DetectionNunes, Maria JoãoMoura, José J.G.Noronha, João PauloBranco, Luís CobraSamhan Arias, Alejandro KhalilSousa, João P.Rouco, CarlosCordas, Cristina M.biomarkersblood analysisLC-MS/MSsamplingstresssweatMedicinaSweat is a potential biological fluid for the non-invasive analytical assessment of diverse molecules, including biomarkers. Notwithstanding, the sampling methodology is critical, and it must be assessed prior to using sweat for clinical diagnosis. In the current work, the analytical methodology was further developed taking into account the sampling step, in view of the identification and level variations of sweat components that have potential to be stress biomarkers using separation by liquid chromatography and detection by tandem mass spectrometry, in order to attain a screening profile of 26 molecules in just one stage. As such, the molecule identification was used as a test for the evaluation of the sampling procedures, including the location on the body, using patches for long-term sampling and vials for direct sampling, through a qualitative approach. From this evaluation it was possible to conclude that the sampling may be performed on the chest or back skin. Additionally, possible interference was evaluated. The long-term sampling with patches can be used under both rest and exercise conditions with variation of the detected molecule’s levels. The direct sampling, using vials, has the advantage of not having interferences but the disadvantage of only being effective after exercise in order to have enough sample for sweat analysisMDPIDepartamento de BioquímicaFacultad de Medicina20222022-04-16research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/713656https://dx.doi.org/10.3390/analytica3020013reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7136562026-06-23T12:46:27Z
dc.title.none.fl_str_mv Evaluation of Sweat-Sampling Procedures for Human Stress-Biomarker Detection
title Evaluation of Sweat-Sampling Procedures for Human Stress-Biomarker Detection
spellingShingle Evaluation of Sweat-Sampling Procedures for Human Stress-Biomarker Detection
Nunes, Maria João
biomarkers
blood analysis
LC-MS/MS
sampling
stress
sweat
Medicina
title_short Evaluation of Sweat-Sampling Procedures for Human Stress-Biomarker Detection
title_full Evaluation of Sweat-Sampling Procedures for Human Stress-Biomarker Detection
title_fullStr Evaluation of Sweat-Sampling Procedures for Human Stress-Biomarker Detection
title_full_unstemmed Evaluation of Sweat-Sampling Procedures for Human Stress-Biomarker Detection
title_sort Evaluation of Sweat-Sampling Procedures for Human Stress-Biomarker Detection
dc.creator.none.fl_str_mv Nunes, Maria João
Moura, José J.G.
Noronha, João Paulo
Branco, Luís Cobra
Samhan Arias, Alejandro Khalil
Sousa, João P.
Rouco, Carlos
Cordas, Cristina M.
author Nunes, Maria João
author_facet Nunes, Maria João
Moura, José J.G.
Noronha, João Paulo
Branco, Luís Cobra
Samhan Arias, Alejandro Khalil
Sousa, João P.
Rouco, Carlos
Cordas, Cristina M.
author_role author
author2 Moura, José J.G.
Noronha, João Paulo
Branco, Luís Cobra
Samhan Arias, Alejandro Khalil
Sousa, João P.
Rouco, Carlos
Cordas, Cristina M.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Bioquímica
Facultad de Medicina
dc.subject.none.fl_str_mv biomarkers
blood analysis
LC-MS/MS
sampling
stress
sweat
Medicina
topic biomarkers
blood analysis
LC-MS/MS
sampling
stress
sweat
Medicina
description Sweat is a potential biological fluid for the non-invasive analytical assessment of diverse molecules, including biomarkers. Notwithstanding, the sampling methodology is critical, and it must be assessed prior to using sweat for clinical diagnosis. In the current work, the analytical methodology was further developed taking into account the sampling step, in view of the identification and level variations of sweat components that have potential to be stress biomarkers using separation by liquid chromatography and detection by tandem mass spectrometry, in order to attain a screening profile of 26 molecules in just one stage. As such, the molecule identification was used as a test for the evaluation of the sampling procedures, including the location on the body, using patches for long-term sampling and vials for direct sampling, through a qualitative approach. From this evaluation it was possible to conclude that the sampling may be performed on the chest or back skin. Additionally, possible interference was evaluated. The long-term sampling with patches can be used under both rest and exercise conditions with variation of the detected molecule’s levels. The direct sampling, using vials, has the advantage of not having interferences but the disadvantage of only being effective after exercise in order to have enough sample for sweat analysis
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-04-16
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/713656
https://dx.doi.org/10.3390/analytica3020013
url http://hdl.handle.net/10486/713656
https://dx.doi.org/10.3390/analytica3020013
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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