Cysteamine improves growth and the GH/IGF axis in gilthead sea bream (Sparus aurata): in vivo and in vitro approaches

Aquaculture is the fastest-growing food production sector and nowadays provides more food than extractive fishing. Studies focused on the understanding of how teleost growth is regulated are essential to improve fish production. Cysteamine (CSH) is a novel feed additive that can improve growth throu...

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Autores: Sánchez-Moya, Albert, Balbuena-Pecino, S., Vélez, Emilio, Perelló-Amorós, Miguel, García-Meilán, Irene, Fontanillas, Ramón, Calduch-Giner, Josep A., Pérez-Sánchez, Jaume, Fernández-Borràs, Jaume, Blasco, Josefina, Gutiérrez, Joaquím
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/348840
Acceso en línea:http://hdl.handle.net/10261/348840
Access Level:acceso abierto
Palabra clave:Cysteamine
Gilthead sea bream
Myocyte
GH
IGF
Somatotropic axis
Aquaculture
Feed additives
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oai_identifier_str oai:digital.csic.es:10261/348840
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Cysteamine improves growth and the GH/IGF axis in gilthead sea bream (Sparus aurata): in vivo and in vitro approaches
title Cysteamine improves growth and the GH/IGF axis in gilthead sea bream (Sparus aurata): in vivo and in vitro approaches
spellingShingle Cysteamine improves growth and the GH/IGF axis in gilthead sea bream (Sparus aurata): in vivo and in vitro approaches
Sánchez-Moya, Albert
Cysteamine
Gilthead sea bream
Myocyte
GH
IGF
Somatotropic axis
Aquaculture
Feed additives
title_short Cysteamine improves growth and the GH/IGF axis in gilthead sea bream (Sparus aurata): in vivo and in vitro approaches
title_full Cysteamine improves growth and the GH/IGF axis in gilthead sea bream (Sparus aurata): in vivo and in vitro approaches
title_fullStr Cysteamine improves growth and the GH/IGF axis in gilthead sea bream (Sparus aurata): in vivo and in vitro approaches
title_full_unstemmed Cysteamine improves growth and the GH/IGF axis in gilthead sea bream (Sparus aurata): in vivo and in vitro approaches
title_sort Cysteamine improves growth and the GH/IGF axis in gilthead sea bream (Sparus aurata): in vivo and in vitro approaches
dc.creator.none.fl_str_mv Sánchez-Moya, Albert
Balbuena-Pecino, S.
Vélez, Emilio
Perelló-Amorós, Miguel
García-Meilán, Irene
Fontanillas, Ramón
Calduch-Giner, Josep A.
Pérez-Sánchez, Jaume
Fernández-Borràs, Jaume
Blasco, Josefina
Gutiérrez, Joaquím
author Sánchez-Moya, Albert
author_facet Sánchez-Moya, Albert
Balbuena-Pecino, S.
Vélez, Emilio
Perelló-Amorós, Miguel
García-Meilán, Irene
Fontanillas, Ramón
Calduch-Giner, Josep A.
Pérez-Sánchez, Jaume
Fernández-Borràs, Jaume
Blasco, Josefina
Gutiérrez, Joaquím
author_role author
author2 Balbuena-Pecino, S.
Vélez, Emilio
Perelló-Amorós, Miguel
García-Meilán, Irene
Fontanillas, Ramón
Calduch-Giner, Josep A.
Pérez-Sánchez, Jaume
Fernández-Borràs, Jaume
Blasco, Josefina
Gutiérrez, Joaquím
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
Generalitat de Catalunya
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Cysteamine
Gilthead sea bream
Myocyte
GH
IGF
Somatotropic axis
Aquaculture
Feed additives
topic Cysteamine
Gilthead sea bream
Myocyte
GH
IGF
Somatotropic axis
Aquaculture
Feed additives
description Aquaculture is the fastest-growing food production sector and nowadays provides more food than extractive fishing. Studies focused on the understanding of how teleost growth is regulated are essential to improve fish production. Cysteamine (CSH) is a novel feed additive that can improve growth through the modulation of the GH/IGF axis; however, the underlying mechanisms and the interaction between tissues are not well understood. This study aimed to investigate the effects of CSH inclusion in diets at 1.65 g/kg of feed for 9 weeks and 1.65 g/kg or 3.3 g/kg for 9 weeks more, on growth performance and the GH/IGF-1 axis in plasma, liver, stomach, and white muscle in gilthead sea bream (Sparus aurata) fingerlings (1.8 ± 0.03 g) and juveniles (14.46 ± 0.68 g). Additionally, the effects of CSH stimulation in primary cultured muscle cells for 4 days on cell viability and GH/IGF axis relative gene expression were evaluated. Results showed that CSH-1.65 improved growth performance by 16% and 26.7% after 9 and 18 weeks, respectively, while CSH-3.3 improved 32.3% after 18 weeks compared to control diet (0 g/kg). However, no significant differences were found between both experimental doses. CSH reduced the plasma levels of GH after 18 weeks and increased the IGF-1 ones after 9 and 18 weeks. Gene expression analysis revealed a significant upregulation of the ghr-1, different igf-1 splice variants, igf-2 and the downregulation of the igf-1ra and b, depending on the tissue and dose. Myocytes stimulated with 200 µM of CSH showed higher cell viability and mRNA levels of ghr1, igf-1b, igf-2 and igf-1rb compared to control (0 µM) in a similar way to white muscle. Overall, CSH improves growth and modulates the GH/IGF-1 axis in vivo and in vitro toward an anabolic status through different synergic ways, revealing CSH as a feasible candidate to be included in fish feed.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
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info:eu-repo/semantics/publishedVersion
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dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/348840
url http://hdl.handle.net/10261/348840
dc.language.none.fl_str_mv Inglés
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Sánchez-Moya, Albert; Balbuena-Pecino, S.; Vélez, Emilio; Perelló-Amorós, Miguel; García-Meilán, Irene; Fontanillas, Ramón; Calduch-Giner, Josep A.; Pérez-Sánchez, Jaume; Fernández-Borràs, Jaume; Blasco, Josefina; Gutiérrez, Joaquím; 2023; Table_1_Cysteamine improves growth and the GH/IGF axis in gilthead sea bream (Sparus aurata): in vivo and in vitro approaches.docx [Dataset]; Frontiers Media; https://doi.org/10.3389/fendo.2023.1211470.s001
https://doi.org/10.3389/fendo.2023.1211470
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dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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spelling Cysteamine improves growth and the GH/IGF axis in gilthead sea bream (Sparus aurata): in vivo and in vitro approachesSánchez-Moya, AlbertBalbuena-Pecino, S.Vélez, EmilioPerelló-Amorós, MiguelGarcía-Meilán, IreneFontanillas, RamónCalduch-Giner, Josep A.Pérez-Sánchez, JaumeFernández-Borràs, JaumeBlasco, JosefinaGutiérrez, JoaquímCysteamineGilthead sea breamMyocyteGHIGFSomatotropic axisAquacultureFeed additivesAquaculture is the fastest-growing food production sector and nowadays provides more food than extractive fishing. Studies focused on the understanding of how teleost growth is regulated are essential to improve fish production. Cysteamine (CSH) is a novel feed additive that can improve growth through the modulation of the GH/IGF axis; however, the underlying mechanisms and the interaction between tissues are not well understood. This study aimed to investigate the effects of CSH inclusion in diets at 1.65 g/kg of feed for 9 weeks and 1.65 g/kg or 3.3 g/kg for 9 weeks more, on growth performance and the GH/IGF-1 axis in plasma, liver, stomach, and white muscle in gilthead sea bream (Sparus aurata) fingerlings (1.8 ± 0.03 g) and juveniles (14.46 ± 0.68 g). Additionally, the effects of CSH stimulation in primary cultured muscle cells for 4 days on cell viability and GH/IGF axis relative gene expression were evaluated. Results showed that CSH-1.65 improved growth performance by 16% and 26.7% after 9 and 18 weeks, respectively, while CSH-3.3 improved 32.3% after 18 weeks compared to control diet (0 g/kg). However, no significant differences were found between both experimental doses. CSH reduced the plasma levels of GH after 18 weeks and increased the IGF-1 ones after 9 and 18 weeks. Gene expression analysis revealed a significant upregulation of the ghr-1, different igf-1 splice variants, igf-2 and the downregulation of the igf-1ra and b, depending on the tissue and dose. Myocytes stimulated with 200 µM of CSH showed higher cell viability and mRNA levels of ghr1, igf-1b, igf-2 and igf-1rb compared to control (0 µM) in a similar way to white muscle. Overall, CSH improves growth and modulates the GH/IGF-1 axis in vivo and in vitro toward an anabolic status through different synergic ways, revealing CSH as a feasible candidate to be included in fish feed.This publication is part of the R+D+i projects AGL2015-70679-R and RTI2018-100757-B-I00 to JG and JB, and AGL2017-89436-R funded by the Spanish “Ministerio de Ciencia e Innovación” (MCIN/AEI/10.13039/501100011033/), the “Xarxa de Refèrencia d’R+D+I en Aqüicultura” (Aqüival Cist-Crec) and the 2017-SGR1574 from the “Generalitat de Catalunya”. SB-P, EV and MP-A, were funded by predoctoral fellowships from the MINECO grants PRE2018-085580, BES-2013-062949 and BES-2016-078697, respectively.Peer reviewedFrontiers MediaMinisterio de Economía y Competitividad (España)Ministerio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)Generalitat de CatalunyaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/348840reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO//AGL2015-70679-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-100757-B-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-89436-Rinfo:eu-repo/grantAgreement/AEI//PRE2018-085580info:eu-repo/grantAgreement/AEI//BES-2016-078697Sánchez-Moya, Albert; Balbuena-Pecino, S.; Vélez, Emilio; Perelló-Amorós, Miguel; García-Meilán, Irene; Fontanillas, Ramón; Calduch-Giner, Josep A.; Pérez-Sánchez, Jaume; Fernández-Borràs, Jaume; Blasco, Josefina; Gutiérrez, Joaquím; 2023; Table_1_Cysteamine improves growth and the GH/IGF axis in gilthead sea bream (Sparus aurata): in vivo and in vitro approaches.docx [Dataset]; Frontiers Media; https://doi.org/10.3389/fendo.2023.1211470.s001https://doi.org/10.3389/fendo.2023.1211470Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3488402026-05-22T06:33:51Z
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