Bioprintable Lung Extracellular Matrix Hydrogel Scaffolds for 3D Culture of Mesenchymal Stromal Cells

Mesenchymal stromal cell (MSC)-based cell therapy in acute respiratory diseases is based on MSC secretion of paracrine factors. Several strategies have proposed to improve this are being explored including pre-conditioning the MSCs prior to administration. We here propose a strategy for improving th...

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Autores: Falcones, Bryan, Sanz Fraile, Héctor, Marhuenda, Esther, Mendizábal, Irene, Cabrera-Aguilera, Ignacio Alfredo, Malandain, Nanthilde, Uriarte, Juan José, Almendros López, Isaac, Navajas Navarro, Daniel, Weiss, Daniel J., Farré Ventura, Ramon, Otero Díaz, Jorge
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Recursos:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/194968
Acesso em linha:https://hdl.handle.net/2445/194968
Access Level:acceso abierto
Palavra-chave:Impressió 3D
Pulmó
Matriu extracel·lular
Enginyeria de teixits
Three-dimensional printing
Lung
Extracellular matrix
Tissue engineering
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spelling Bioprintable Lung Extracellular Matrix Hydrogel Scaffolds for 3D Culture of Mesenchymal Stromal CellsFalcones, BryanSanz Fraile, HéctorMarhuenda, EstherMendizábal, IreneCabrera-Aguilera, Ignacio AlfredoMalandain, NanthildeUriarte, Juan JoséAlmendros López, IsaacNavajas Navarro, DanielWeiss, Daniel J.Farré Ventura, RamonOtero Díaz, JorgeImpressió 3DPulmóMatriu extracel·lularEnginyeria de teixitsThree-dimensional printingLungExtracellular matrixTissue engineeringMesenchymal stromal cell (MSC)-based cell therapy in acute respiratory diseases is based on MSC secretion of paracrine factors. Several strategies have proposed to improve this are being explored including pre-conditioning the MSCs prior to administration. We here propose a strategy for improving the therapeutic efficacy of MSCs based on cell preconditioning by growing them in native extracellular matrix (ECM) derived from the lung. To this end, a bioink with tunable stiffness based on decellularized porcine lung ECM hydrogels was developed and characterized. The bioink was suitable for 3D culturing of lung-resident MSCs without the need for additional chemical or physical crosslinking. MSCs showed good viability, and contraction assays showed the existence of cell-matrix interactions in the bioprinted scaffolds. Adhesion capacity and length of the focal adhesions formed were increased for the cells cultured within the lung hydrogel scaffolds. Also, there was more than a 20-fold increase of the expression of the CXCR4 receptor in the 3D-cultured cells compared to the cells cultured in plastic. Secretion of cytokines when cultured in an in vitro model of lung injury showed a decreased secretion of pro-inflammatory mediators for the cells cultured in the 3D scaffolds. Moreover, the morphology of the harvested cells was markedly different with respect to conventionally (2D) cultured MSCs. In conclusion, the developed bioink can be used to bioprint structures aimed to improve preconditioning MSCs for therapeutic purposes.MDPI2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/194968Articles publicats en revistes (Biomedicina)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/polym13142350Polymers, 2021, vol. 13, num. 14, p. 2350https://doi.org/10.3390/polym13142350cc-by (c) Falcones, Bryan et al., 2021https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1949682026-05-27T06:46:51Z
dc.title.none.fl_str_mv Bioprintable Lung Extracellular Matrix Hydrogel Scaffolds for 3D Culture of Mesenchymal Stromal Cells
title Bioprintable Lung Extracellular Matrix Hydrogel Scaffolds for 3D Culture of Mesenchymal Stromal Cells
spellingShingle Bioprintable Lung Extracellular Matrix Hydrogel Scaffolds for 3D Culture of Mesenchymal Stromal Cells
Falcones, Bryan
Impressió 3D
Pulmó
Matriu extracel·lular
Enginyeria de teixits
Three-dimensional printing
Lung
Extracellular matrix
Tissue engineering
title_short Bioprintable Lung Extracellular Matrix Hydrogel Scaffolds for 3D Culture of Mesenchymal Stromal Cells
title_full Bioprintable Lung Extracellular Matrix Hydrogel Scaffolds for 3D Culture of Mesenchymal Stromal Cells
title_fullStr Bioprintable Lung Extracellular Matrix Hydrogel Scaffolds for 3D Culture of Mesenchymal Stromal Cells
title_full_unstemmed Bioprintable Lung Extracellular Matrix Hydrogel Scaffolds for 3D Culture of Mesenchymal Stromal Cells
title_sort Bioprintable Lung Extracellular Matrix Hydrogel Scaffolds for 3D Culture of Mesenchymal Stromal Cells
dc.creator.none.fl_str_mv Falcones, Bryan
Sanz Fraile, Héctor
Marhuenda, Esther
Mendizábal, Irene
Cabrera-Aguilera, Ignacio Alfredo
Malandain, Nanthilde
Uriarte, Juan José
Almendros López, Isaac
Navajas Navarro, Daniel
Weiss, Daniel J.
Farré Ventura, Ramon
Otero Díaz, Jorge
author Falcones, Bryan
author_facet Falcones, Bryan
Sanz Fraile, Héctor
Marhuenda, Esther
Mendizábal, Irene
Cabrera-Aguilera, Ignacio Alfredo
Malandain, Nanthilde
Uriarte, Juan José
Almendros López, Isaac
Navajas Navarro, Daniel
Weiss, Daniel J.
Farré Ventura, Ramon
Otero Díaz, Jorge
author_role author
author2 Sanz Fraile, Héctor
Marhuenda, Esther
Mendizábal, Irene
Cabrera-Aguilera, Ignacio Alfredo
Malandain, Nanthilde
Uriarte, Juan José
Almendros López, Isaac
Navajas Navarro, Daniel
Weiss, Daniel J.
Farré Ventura, Ramon
Otero Díaz, Jorge
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Impressió 3D
Pulmó
Matriu extracel·lular
Enginyeria de teixits
Three-dimensional printing
Lung
Extracellular matrix
Tissue engineering
topic Impressió 3D
Pulmó
Matriu extracel·lular
Enginyeria de teixits
Three-dimensional printing
Lung
Extracellular matrix
Tissue engineering
description Mesenchymal stromal cell (MSC)-based cell therapy in acute respiratory diseases is based on MSC secretion of paracrine factors. Several strategies have proposed to improve this are being explored including pre-conditioning the MSCs prior to administration. We here propose a strategy for improving the therapeutic efficacy of MSCs based on cell preconditioning by growing them in native extracellular matrix (ECM) derived from the lung. To this end, a bioink with tunable stiffness based on decellularized porcine lung ECM hydrogels was developed and characterized. The bioink was suitable for 3D culturing of lung-resident MSCs without the need for additional chemical or physical crosslinking. MSCs showed good viability, and contraction assays showed the existence of cell-matrix interactions in the bioprinted scaffolds. Adhesion capacity and length of the focal adhesions formed were increased for the cells cultured within the lung hydrogel scaffolds. Also, there was more than a 20-fold increase of the expression of the CXCR4 receptor in the 3D-cultured cells compared to the cells cultured in plastic. Secretion of cytokines when cultured in an in vitro model of lung injury showed a decreased secretion of pro-inflammatory mediators for the cells cultured in the 3D scaffolds. Moreover, the morphology of the harvested cells was markedly different with respect to conventionally (2D) cultured MSCs. In conclusion, the developed bioink can be used to bioprint structures aimed to improve preconditioning MSCs for therapeutic purposes.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/194968
url https://hdl.handle.net/2445/194968
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/polym13142350
Polymers, 2021, vol. 13, num. 14, p. 2350
https://doi.org/10.3390/polym13142350
dc.rights.none.fl_str_mv cc-by (c) Falcones, Bryan et al., 2021
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Falcones, Bryan et al., 2021
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Biomedicina)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869408810059169792
score 15,198674

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