Potentiometric detection of ATP based on the transmembrane proton gradient generated by ATPase reconstituted on a gold electrode

[EN] Adenosine triphosphate (ATP) is a key molecule as energy vector for living organisms, therefore its detection reveals the presence of microbial colonies. Environments where the existence of microbial pathogens suppose a health hazard can benefit from real time monitoring of such molecule. We re...

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Detalles Bibliográficos
Autores: García-Molina, Gabriel, Natale, Paolo, Valenzuela, Laura, Álvarez-Malmagro, Julia, Gutiérrez-Sánchez, Cristina, Iglesias Juez, Ana, López-Montero, Iván, Vélez, Marisela, Pita, Marcos, López de Lacey, Antonio
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/216241
Acceso en línea:http://hdl.handle.net/10261/216241
Access Level:acceso abierto
Palabra clave:ATP
ATP-synthase
Potentiometric biosensor
Biomimetic membranes
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spelling Potentiometric detection of ATP based on the transmembrane proton gradient generated by ATPase reconstituted on a gold electrodeGarcía-Molina, GabrielNatale, PaoloValenzuela, LauraÁlvarez-Malmagro, JuliaGutiérrez-Sánchez, CristinaIglesias Juez, AnaLópez-Montero, IvánVélez, MariselaPita, MarcosLópez de Lacey, AntonioATPATP-synthasePotentiometric biosensorBiomimetic membranes[EN] Adenosine triphosphate (ATP) is a key molecule as energy vector for living organisms, therefore its detection reveals the presence of microbial colonies. Environments where the existence of microbial pathogens suppose a health hazard can benefit from real time monitoring of such molecule. We report a potentiometric biosensor based on ATP-synthase from Escherichia coli reconstituted in a floating phospholipid bilayer over gold electrodes modified with a 4-aminothiophenol self-assembled monolayer. The use of a pH-dependent redox probe on the electrode surface allows a simple, specific and reliable on site determination of ATP concentration from 1 μM to 1 mM. The broad range ATP biosensor can offer an alternative way of measuring in a few minutes the presence of microbial contamination.A.L.D, M.P. and M.V. thank MCIU/AEI/FEDER, EU for funding project RTI2018-095090-B-I00. L.V. and A.I.J. thank Fundación Ramón Areces for financial support through the project OTR02666 and Prof. Rosal García (University of Alcalá) for the use of his laboratory facilities. I.L.-M. and M.V. thank the TECNOLOGÍAS 2018 program funded by the Regional Government of Madrid (Grant S2018/BAA-4403 SINOXPHOS-CM). G.G.M. thanks MCIU/AEI/FEDER, EU for a predoctoral contract (BES-2016-078815).ElsevierMinisterio de Ciencia, Innovación y Universidades (España)Fundación Ramón ArecesComunidad de MadridMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/216241reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095090-B-I00info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2015-71290-RS2018/BAA-4403/SINOXPHOS-CMhttp://dx.doi.org/10.1016/j.bioelechem.2020.107490Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2162412026-05-22T06:33:51Z
dc.title.none.fl_str_mv Potentiometric detection of ATP based on the transmembrane proton gradient generated by ATPase reconstituted on a gold electrode
title Potentiometric detection of ATP based on the transmembrane proton gradient generated by ATPase reconstituted on a gold electrode
spellingShingle Potentiometric detection of ATP based on the transmembrane proton gradient generated by ATPase reconstituted on a gold electrode
García-Molina, Gabriel
ATP
ATP-synthase
Potentiometric biosensor
Biomimetic membranes
title_short Potentiometric detection of ATP based on the transmembrane proton gradient generated by ATPase reconstituted on a gold electrode
title_full Potentiometric detection of ATP based on the transmembrane proton gradient generated by ATPase reconstituted on a gold electrode
title_fullStr Potentiometric detection of ATP based on the transmembrane proton gradient generated by ATPase reconstituted on a gold electrode
title_full_unstemmed Potentiometric detection of ATP based on the transmembrane proton gradient generated by ATPase reconstituted on a gold electrode
title_sort Potentiometric detection of ATP based on the transmembrane proton gradient generated by ATPase reconstituted on a gold electrode
dc.creator.none.fl_str_mv García-Molina, Gabriel
Natale, Paolo
Valenzuela, Laura
Álvarez-Malmagro, Julia
Gutiérrez-Sánchez, Cristina
Iglesias Juez, Ana
López-Montero, Iván
Vélez, Marisela
Pita, Marcos
López de Lacey, Antonio
author García-Molina, Gabriel
author_facet García-Molina, Gabriel
Natale, Paolo
Valenzuela, Laura
Álvarez-Malmagro, Julia
Gutiérrez-Sánchez, Cristina
Iglesias Juez, Ana
López-Montero, Iván
Vélez, Marisela
Pita, Marcos
López de Lacey, Antonio
author_role author
author2 Natale, Paolo
Valenzuela, Laura
Álvarez-Malmagro, Julia
Gutiérrez-Sánchez, Cristina
Iglesias Juez, Ana
López-Montero, Iván
Vélez, Marisela
Pita, Marcos
López de Lacey, Antonio
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Fundación Ramón Areces
Comunidad de Madrid
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv ATP
ATP-synthase
Potentiometric biosensor
Biomimetic membranes
topic ATP
ATP-synthase
Potentiometric biosensor
Biomimetic membranes
description [EN] Adenosine triphosphate (ATP) is a key molecule as energy vector for living organisms, therefore its detection reveals the presence of microbial colonies. Environments where the existence of microbial pathogens suppose a health hazard can benefit from real time monitoring of such molecule. We report a potentiometric biosensor based on ATP-synthase from Escherichia coli reconstituted in a floating phospholipid bilayer over gold electrodes modified with a 4-aminothiophenol self-assembled monolayer. The use of a pH-dependent redox probe on the electrode surface allows a simple, specific and reliable on site determination of ATP concentration from 1 μM to 1 mM. The broad range ATP biosensor can offer an alternative way of measuring in a few minutes the presence of microbial contamination.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/216241
url http://hdl.handle.net/10261/216241
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095090-B-I00
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2015-71290-R
S2018/BAA-4403/SINOXPHOS-CM
http://dx.doi.org/10.1016/j.bioelechem.2020.107490
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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