Novel nanofibrous dressings containing rhEGF and Aloe vera for wound healing applications

Nanofibrous membranes produced by electrospinning possess a large surface area-to-volume ratio, which mimics the three-dimensional structure of the extracellular matrix. Thus, nanofibrous dressings are a promising alternative for chronic wound healing, since they can replace the natural ECM until it...

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Autores: García Orúe, Itxaso, Gainza Luzea, Garazi, Gutiérrez, Francisco Borja, Aguirre, José Javier, Evora, Carmen, Pedraz Muñoz, José Luis, Hernández Martín, Rosa María, Delgado, Araceli, Igartua Olaechea, Manuela
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/71718
Acceso en línea:http://hdl.handle.net/10810/71718
Access Level:acceso abierto
Palabra clave:wound healing
electrospinning
wound dressing
rhEGF
growth factors
aloe vera
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spelling Novel nanofibrous dressings containing rhEGF and Aloe vera for wound healing applicationsGarcía Orúe, ItxasoGainza Luzea, GaraziGutiérrez, Francisco BorjaAguirre, José JavierEvora, CarmenPedraz Muñoz, José LuisHernández Martín, Rosa MaríaDelgado, AraceliIgartua Olaechea, Manuelawound healingelectrospinningwound dressingrhEGFgrowth factorsaloe veraNanofibrous membranes produced by electrospinning possess a large surface area-to-volume ratio, which mimics the three-dimensional structure of the extracellular matrix. Thus, nanofibrous dressings are a promising alternative for chronic wound healing, since they can replace the natural ECM until it is repaired. Therefore, in this study we have developed a PLGA nanofibrous membrane that contains recombinant human Epidermal Growth Factor (rhEGF) and Aloe vera (AV) extract. Both of them promote wound healing, as EGF is a wound healing mediator and AV stimulates the proliferation and activity of fibroblast. The obtained membranes were composed of uniform and randomly oriented fibers with an average diameter of 356.03±112.05 nm, they presented a porosity of 87.92±11.96% and the amount of rhEGF was 9.76±1.75 µg/mg. The in vitro viability assay demonstrated that the membranes containing rhEGF and AV improved fibroblast proliferation, revealing the beneficial effect of the combination. Furthermore, these membranes accelerated significantly wound closure and reepithelisation in an in vivo full thickness wound healing assay carried out in db/db mice. Overall, these findings demonstrated the potential of PLGA nanofibers containing rhEGF and AV for the treatment of chronic wounds.I. García-Orue thanks the Basque Government for the fellowship grant. The authors thank for technical and human support provided by SGIker of UPV/EHU and European funding (ERDF and ESF). This project has been funded by the Basque Government (ELKARTEK 2015, Nanoplatform, KK-2015/0000036). In addition, it has been partially supported by the University of the Basque Country (UPV/EHU) (UFI 11/32). Authors also wish to thank the intellectual and technical assistance from the ICTS “NANBIOSIS”, more specifically by the Drug Formulation Unit (U10) of the CIBER-BBN at the UPV/EHU.Elsevier202520252016info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/71718reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoIngléshttps://doi.org/10.1016/j.ijpharm.2016.11.006info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/© 2016 Elsevier under CC BY-NC-ND licenseoai:addi.ehu.eus:10810/717182026-06-18T09:23:17Z
dc.title.none.fl_str_mv Novel nanofibrous dressings containing rhEGF and Aloe vera for wound healing applications
title Novel nanofibrous dressings containing rhEGF and Aloe vera for wound healing applications
spellingShingle Novel nanofibrous dressings containing rhEGF and Aloe vera for wound healing applications
García Orúe, Itxaso
wound healing
electrospinning
wound dressing
rhEGF
growth factors
aloe vera
title_short Novel nanofibrous dressings containing rhEGF and Aloe vera for wound healing applications
title_full Novel nanofibrous dressings containing rhEGF and Aloe vera for wound healing applications
title_fullStr Novel nanofibrous dressings containing rhEGF and Aloe vera for wound healing applications
title_full_unstemmed Novel nanofibrous dressings containing rhEGF and Aloe vera for wound healing applications
title_sort Novel nanofibrous dressings containing rhEGF and Aloe vera for wound healing applications
dc.creator.none.fl_str_mv García Orúe, Itxaso
Gainza Luzea, Garazi
Gutiérrez, Francisco Borja
Aguirre, José Javier
Evora, Carmen
Pedraz Muñoz, José Luis
Hernández Martín, Rosa María
Delgado, Araceli
Igartua Olaechea, Manuela
author García Orúe, Itxaso
author_facet García Orúe, Itxaso
Gainza Luzea, Garazi
Gutiérrez, Francisco Borja
Aguirre, José Javier
Evora, Carmen
Pedraz Muñoz, José Luis
Hernández Martín, Rosa María
Delgado, Araceli
Igartua Olaechea, Manuela
author_role author
author2 Gainza Luzea, Garazi
Gutiérrez, Francisco Borja
Aguirre, José Javier
Evora, Carmen
Pedraz Muñoz, José Luis
Hernández Martín, Rosa María
Delgado, Araceli
Igartua Olaechea, Manuela
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv wound healing
electrospinning
wound dressing
rhEGF
growth factors
aloe vera
topic wound healing
electrospinning
wound dressing
rhEGF
growth factors
aloe vera
description Nanofibrous membranes produced by electrospinning possess a large surface area-to-volume ratio, which mimics the three-dimensional structure of the extracellular matrix. Thus, nanofibrous dressings are a promising alternative for chronic wound healing, since they can replace the natural ECM until it is repaired. Therefore, in this study we have developed a PLGA nanofibrous membrane that contains recombinant human Epidermal Growth Factor (rhEGF) and Aloe vera (AV) extract. Both of them promote wound healing, as EGF is a wound healing mediator and AV stimulates the proliferation and activity of fibroblast. The obtained membranes were composed of uniform and randomly oriented fibers with an average diameter of 356.03±112.05 nm, they presented a porosity of 87.92±11.96% and the amount of rhEGF was 9.76±1.75 µg/mg. The in vitro viability assay demonstrated that the membranes containing rhEGF and AV improved fibroblast proliferation, revealing the beneficial effect of the combination. Furthermore, these membranes accelerated significantly wound closure and reepithelisation in an in vivo full thickness wound healing assay carried out in db/db mice. Overall, these findings demonstrated the potential of PLGA nanofibers containing rhEGF and AV for the treatment of chronic wounds.
publishDate 2016
dc.date.none.fl_str_mv 2016
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/71718
url http://hdl.handle.net/10810/71718
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.ijpharm.2016.11.006
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
© 2016 Elsevier under CC BY-NC-ND license
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
© 2016 Elsevier under CC BY-NC-ND license
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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