Novel Targets Regulating the Role of Endothelial Cells and Angiogenesis after Infarction: A RNA Sequencing Analysis

Endothelial cells (ECs) are a key target for cardioprotection due to their role in preserving cardiac microvasculature and homeostasis after myocardial infarction (MI). Our goal is to identify the genes involved in post-MI EC proliferation, EC apoptosis, and angiogenesis regulation via RNA-sequencin...

Descripción completa

Detalles Bibliográficos
Autores: Ortega, Maria, Molina-Garcia, Tamara, Gavara, Jose, de Dios, Elena, Perez-Sole, Nerea, Marcos-Garces, Victor, Chorro, Francisco J, Rios-Navarro, Cesar, Ruiz-Sauri, Amparo, Bodi, Vicente
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:INCLIVA
Repositorio:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
OAI Identifier:oai:incliva.fundanetsuite.com:p17814
Acceso en línea:https://incliva.portalinvestigacion.com/publicaciones/17814
Access Level:acceso abierto
Palabra clave:myocardial infarction
angiogenesis
endothelial cells
RNA sequencing
id ES_5b47ec0bed2c9f8c5edea1ec3cc4e14c
oai_identifier_str oai:incliva.fundanetsuite.com:p17814
network_acronym_str ES
network_name_str España
repository_id_str
spelling Novel Targets Regulating the Role of Endothelial Cells and Angiogenesis after Infarction: A RNA Sequencing AnalysisOrtega, MariaMolina-Garcia, TamaraGavara, Josede Dios, ElenaPerez-Sole, NereaMarcos-Garces, VictorChorro, Francisco JRios-Navarro, CesarRuiz-Sauri, AmparoBodi, Vicentemyocardial infarctionangiogenesisendothelial cellsRNA sequencingEndothelial cells (ECs) are a key target for cardioprotection due to their role in preserving cardiac microvasculature and homeostasis after myocardial infarction (MI). Our goal is to identify the genes involved in post-MI EC proliferation, EC apoptosis, and angiogenesis regulation via RNA-sequencing transcriptomic datasets. Using eight studies from the Gene Expression Omnibus, RNA-sequencing data from 92 mice submitted to different times of coronary ischemia or sham were chosen. Functional enrichment analysis was performed based on gene ontology biological processes (BPs). Apoptosis-related BPs are activated up to day 3 after ischemia onset, whereas endothelial proliferation occurs from day 3 onwards, including an overrepresentation of up to 37 genes. Endothelial apoptosis post-MI is triggered via both the extrinsic and intrinsic signaling pathways, as reflected by the overrepresentation of 13 and 2 specific genes, respectively. BPs implicated in new vessel formation are upregulated soon after ischemia onset, whilst the mechanisms aiming at angiogenesis repression can be detected at day 3. Overall, 51 pro-angiogenic and 29 anti-angiogenic factors displayed altered transcriptomic expression post-MI. This is the first study using RNA sequencing datasets to evaluate the genes participating in post-MI endothelium physiology and angiogenesis regulation. These novel data could lay the groundwork to advance understanding of the implication of ECs after MI.MDPI2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://incliva.portalinvestigacion.com/publicaciones/17814INTERNATIONAL JOURNAL OF MOLECULAR SCIENCESISSN: 16616596ISSNe: 14220067reponame:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVAinstname:INCLIVAInglésinfo:eu-repo/semantics/openAccessoai:incliva.fundanetsuite.com:p178142026-06-07T16:35:31Z
dc.title.none.fl_str_mv Novel Targets Regulating the Role of Endothelial Cells and Angiogenesis after Infarction: A RNA Sequencing Analysis
title Novel Targets Regulating the Role of Endothelial Cells and Angiogenesis after Infarction: A RNA Sequencing Analysis
spellingShingle Novel Targets Regulating the Role of Endothelial Cells and Angiogenesis after Infarction: A RNA Sequencing Analysis
Ortega, Maria
myocardial infarction
angiogenesis
endothelial cells
RNA sequencing
title_short Novel Targets Regulating the Role of Endothelial Cells and Angiogenesis after Infarction: A RNA Sequencing Analysis
title_full Novel Targets Regulating the Role of Endothelial Cells and Angiogenesis after Infarction: A RNA Sequencing Analysis
title_fullStr Novel Targets Regulating the Role of Endothelial Cells and Angiogenesis after Infarction: A RNA Sequencing Analysis
title_full_unstemmed Novel Targets Regulating the Role of Endothelial Cells and Angiogenesis after Infarction: A RNA Sequencing Analysis
title_sort Novel Targets Regulating the Role of Endothelial Cells and Angiogenesis after Infarction: A RNA Sequencing Analysis
dc.creator.none.fl_str_mv Ortega, Maria
Molina-Garcia, Tamara
Gavara, Jose
de Dios, Elena
Perez-Sole, Nerea
Marcos-Garces, Victor
Chorro, Francisco J
Rios-Navarro, Cesar
Ruiz-Sauri, Amparo
Bodi, Vicente
author Ortega, Maria
author_facet Ortega, Maria
Molina-Garcia, Tamara
Gavara, Jose
de Dios, Elena
Perez-Sole, Nerea
Marcos-Garces, Victor
Chorro, Francisco J
Rios-Navarro, Cesar
Ruiz-Sauri, Amparo
Bodi, Vicente
author_role author
author2 Molina-Garcia, Tamara
Gavara, Jose
de Dios, Elena
Perez-Sole, Nerea
Marcos-Garces, Victor
Chorro, Francisco J
Rios-Navarro, Cesar
Ruiz-Sauri, Amparo
Bodi, Vicente
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv myocardial infarction
angiogenesis
endothelial cells
RNA sequencing
topic myocardial infarction
angiogenesis
endothelial cells
RNA sequencing
description Endothelial cells (ECs) are a key target for cardioprotection due to their role in preserving cardiac microvasculature and homeostasis after myocardial infarction (MI). Our goal is to identify the genes involved in post-MI EC proliferation, EC apoptosis, and angiogenesis regulation via RNA-sequencing transcriptomic datasets. Using eight studies from the Gene Expression Omnibus, RNA-sequencing data from 92 mice submitted to different times of coronary ischemia or sham were chosen. Functional enrichment analysis was performed based on gene ontology biological processes (BPs). Apoptosis-related BPs are activated up to day 3 after ischemia onset, whereas endothelial proliferation occurs from day 3 onwards, including an overrepresentation of up to 37 genes. Endothelial apoptosis post-MI is triggered via both the extrinsic and intrinsic signaling pathways, as reflected by the overrepresentation of 13 and 2 specific genes, respectively. BPs implicated in new vessel formation are upregulated soon after ischemia onset, whilst the mechanisms aiming at angiogenesis repression can be detected at day 3. Overall, 51 pro-angiogenic and 29 anti-angiogenic factors displayed altered transcriptomic expression post-MI. This is the first study using RNA sequencing datasets to evaluate the genes participating in post-MI endothelium physiology and angiogenesis regulation. These novel data could lay the groundwork to advance understanding of the implication of ECs after MI.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://incliva.portalinvestigacion.com/publicaciones/17814
url https://incliva.portalinvestigacion.com/publicaciones/17814
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN: 16616596
ISSNe: 14220067
reponame:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
instname:INCLIVA
instname_str INCLIVA
reponame_str r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
collection r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869408787339673600
score 15,81155

Similares en LA Referencia