Identificación de una molécula similar a la fibronectina en el bivalvo marino Pecten maximus (L., 1758) y su hiperacumulación en el compartimento femenino de la gónada

The present paper is the first report on the existence in marine scallops of a protein with properties similar to those of mammalian fibronectin (FN), henceforth called a scallop fibronectinlike polypeptide (sFN-LP). We describe the results of the biochemical and immunochemical identification of sFN...

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Detalles Bibliográficos
Autores: Paz, M.ª, Ruiz, M.F., Sánchez, L., Mikhailov, A.
Tipo de recurso: artículo
Fecha de publicación:2002
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/320002
Acceso en línea:http://hdl.handle.net/10261/320002
Access Level:acceso abierto
Palabra clave:Fibronectina
Medio Marino
pectínidos
gónada
diferenciación sexual
Descripción
Sumario:The present paper is the first report on the existence in marine scallops of a protein with properties similar to those of mammalian fibronectin (FN), henceforth called a scallop fibronectinlike polypeptide (sFN-LP). We describe the results of the biochemical and immunochemical identification of sFN-LP in mature gonad tissue of the simultaneous hermaphroditic species Pecten maximus (L., 1758). sFN-LP is similar to mammalian FN in the following respects: (1) it is a dimeric polypeptide with subunits of approx. 200 kDa; (2) it is recognised by antibodies against human FN on Western-blots; and (3) it is located in the basement membranes of follicles, the connective-tissue matrix, and the integument of the gonad. In the P. maximus female gonad compartment, sFN-LP is highly accumulated in the connective-tissue fibrilar network but is not detected in oocytes. In the male gonad tissue, only trace amounts of sFN-LP were detected.