Identification of antihypertensive peptides in nutraceuticals by capillary electrophoresis-mass spectrometry

We present capillary electrophoresis-mass spectrometry (CE-MS) in combination with advanced chemometric tools for the analysis of bioactive compounds in food, in particular for the identification of antihypertensive peptides in a nutraceutical derived from a bovine milk protein hydrolysate. Differen...

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Detalles Bibliográficos
Autores: Benavente Moreno, Fernando J. (Julián), Peró Gascón, Roger, Pont Villanueva, Laura, Jaumot Soler, Joaquim, Barbosa Torralbo, José, Sanz Nebot, María Victoria
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/151389
Acceso en línea:https://hdl.handle.net/2445/151389
Access Level:acceso abierto
Palabra clave:Pèptids
Electroforesi capil·lar
Quimiometria
Espectrometria de masses
Peptides
Capillary electrophoresis
Chemometrics
Mass spectrometry
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spelling Identification of antihypertensive peptides in nutraceuticals by capillary electrophoresis-mass spectrometryBenavente Moreno, Fernando J. (Julián)Peró Gascón, RogerPont Villanueva, LauraJaumot Soler, JoaquimBarbosa Torralbo, JoséSanz Nebot, María VictoriaPèptidsElectroforesi capil·larQuimiometriaEspectrometria de massesPeptidesCapillary electrophoresisChemometricsMass spectrometryWe present capillary electrophoresis-mass spectrometry (CE-MS) in combination with advanced chemometric tools for the analysis of bioactive compounds in food, in particular for the identification of antihypertensive peptides in a nutraceutical derived from a bovine milk protein hydrolysate. Different extracts of the nutraceutical were analyzed by CE-MS, and the electropherograms were processed using a novel data analysis workflow that included regions of interest (ROIs) compression and multivariate curve resolution alternating least squares (MCR-ALS). MCR-ALS permitted the description of the nutraceutical extract as ten characteristic components with their electrophoretic profiles and mass spectra. Twenty-two compounds were tentatively identified as antihypertensive bovine casein fragments through a mass search in a database of bioactive peptides. The identity of 17 antihypertensive peptides was reliably confirmed by capillary electrophoresis-tandem mass spectrometry. The proposed analytical approach demonstrated the potential to obtain a characteristic and activity-related fingerprint for quality control and authentication of the antihypertensive nutraceutical.Elsevier B.V.2020202020182020info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion9 p.application/pdfhttps://hdl.handle.net/2445/151389Articles publicats en revistes (Enginyeria Química i Química Analítica)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1016/j.chroma.2018.10.018Journal of Chromatography A, 2018, vol. 1579, p. 129-137https://doi.org/10.1016/j.chroma.2018.10.018cc-by-nc-nd (c) Elsevier B.V., 2018http://creativecommons.org/licenses/by-nc-nd/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1513892026-05-29T05:05:01Z
dc.title.none.fl_str_mv Identification of antihypertensive peptides in nutraceuticals by capillary electrophoresis-mass spectrometry
title Identification of antihypertensive peptides in nutraceuticals by capillary electrophoresis-mass spectrometry
spellingShingle Identification of antihypertensive peptides in nutraceuticals by capillary electrophoresis-mass spectrometry
Benavente Moreno, Fernando J. (Julián)
Pèptids
Electroforesi capil·lar
Quimiometria
Espectrometria de masses
Peptides
Capillary electrophoresis
Chemometrics
Mass spectrometry
title_short Identification of antihypertensive peptides in nutraceuticals by capillary electrophoresis-mass spectrometry
title_full Identification of antihypertensive peptides in nutraceuticals by capillary electrophoresis-mass spectrometry
title_fullStr Identification of antihypertensive peptides in nutraceuticals by capillary electrophoresis-mass spectrometry
title_full_unstemmed Identification of antihypertensive peptides in nutraceuticals by capillary electrophoresis-mass spectrometry
title_sort Identification of antihypertensive peptides in nutraceuticals by capillary electrophoresis-mass spectrometry
dc.creator.none.fl_str_mv Benavente Moreno, Fernando J. (Julián)
Peró Gascón, Roger
Pont Villanueva, Laura
Jaumot Soler, Joaquim
Barbosa Torralbo, José
Sanz Nebot, María Victoria
author Benavente Moreno, Fernando J. (Julián)
author_facet Benavente Moreno, Fernando J. (Julián)
Peró Gascón, Roger
Pont Villanueva, Laura
Jaumot Soler, Joaquim
Barbosa Torralbo, José
Sanz Nebot, María Victoria
author_role author
author2 Peró Gascón, Roger
Pont Villanueva, Laura
Jaumot Soler, Joaquim
Barbosa Torralbo, José
Sanz Nebot, María Victoria
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Pèptids
Electroforesi capil·lar
Quimiometria
Espectrometria de masses
Peptides
Capillary electrophoresis
Chemometrics
Mass spectrometry
topic Pèptids
Electroforesi capil·lar
Quimiometria
Espectrometria de masses
Peptides
Capillary electrophoresis
Chemometrics
Mass spectrometry
description We present capillary electrophoresis-mass spectrometry (CE-MS) in combination with advanced chemometric tools for the analysis of bioactive compounds in food, in particular for the identification of antihypertensive peptides in a nutraceutical derived from a bovine milk protein hydrolysate. Different extracts of the nutraceutical were analyzed by CE-MS, and the electropherograms were processed using a novel data analysis workflow that included regions of interest (ROIs) compression and multivariate curve resolution alternating least squares (MCR-ALS). MCR-ALS permitted the description of the nutraceutical extract as ten characteristic components with their electrophoretic profiles and mass spectra. Twenty-two compounds were tentatively identified as antihypertensive bovine casein fragments through a mass search in a database of bioactive peptides. The identity of 17 antihypertensive peptides was reliably confirmed by capillary electrophoresis-tandem mass spectrometry. The proposed analytical approach demonstrated the potential to obtain a characteristic and activity-related fingerprint for quality control and authentication of the antihypertensive nutraceutical.
publishDate 2018
dc.date.none.fl_str_mv 2018
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/151389
url https://hdl.handle.net/2445/151389
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/j.chroma.2018.10.018
Journal of Chromatography A, 2018, vol. 1579, p. 129-137
https://doi.org/10.1016/j.chroma.2018.10.018
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Elsevier B.V., 2018
http://creativecommons.org/licenses/by-nc-nd/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Elsevier B.V., 2018
http://creativecommons.org/licenses/by-nc-nd/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Enginyeria Química i Química Analítica)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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