Serological and molecular survey of Leishmania infection in dogs from Venezuela

Venezuela is a country where human and canine leishmaniosis due to Leishmania infantum, Leishmania braziliensis and other Leishmania spp. is endemic. However, only limited data is available on canine Leishmania infection in Venezuela. The aim of this cross-sectional study was to evaluate the prevale...

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Autores: Kalú Rivas, Aruani, Alcover Amengual, Maria Magdalena, Martínez-Orellana, Pamela, Montserrat-Sangrà, Sara, Nachum-Biala, Yaarit, Fisa Saladrigas, Roser, Riera Lizandra, Ma. Cristina, Baneth, Gad, Solano-Gallego, Laia
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/169250
Acesso em linha:https://hdl.handle.net/2445/169250
Access Level:acceso abierto
Palavra-chave:Leishmaniosi
Gossos
Veneçuela
Malalties infeccioses en els animals
Leishmaniasis
Dogs
Venezuela
Communicable diseases in animals
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spelling Serological and molecular survey of Leishmania infection in dogs from VenezuelaKalú Rivas, AruaniAlcover Amengual, Maria MagdalenaMartínez-Orellana, PamelaMontserrat-Sangrà, SaraNachum-Biala, YaaritFisa Saladrigas, RoserRiera Lizandra, Ma. CristinaBaneth, GadSolano-Gallego, LaiaLeishmaniosiGossosVeneçuelaMalalties infeccioses en els animalsLeishmaniasisDogsVenezuelaCommunicable diseases in animalsVenezuela is a country where human and canine leishmaniosis due to Leishmania infantum, Leishmania braziliensis and other Leishmania spp. is endemic. However, only limited data is available on canine Leishmania infection in Venezuela. The aim of this cross-sectional study was to evaluate the prevalence of Leishmania infection in dogs (n = 152) from the states of Lara (n = 91) and Yaracuy (n = 61) in Venezuela by means of serological and molecular methods. Physical examination was performed and blood samples were collected from all dogs. Serology for antibodies reactive with L. infantum and L. braziliensis antigens was assessed by the enzyme-linked immunosorbent assay (ELISA) and detection of Leishmania DNA from blood samples was evaluated by kinetoplast Leishmania real-time polymerase chain reaction (RT-PCR). In addition, Leishmania internal transcribed spacer (ITS-1) RT-PCR was performed on the samples positive by kinetoplast RT-PCR. The prevalence of Leishmania infection based on serological and/or molecular techniques was 11.8%. The seroprevalence for L. infantum and L. braziliensis antigens were 2.1% (3/144) and 8.3% (12/144), respectively. All dogs from the state of Yaracuy were serologically negative to L. infantum while 4.6% (4/86) of the dogs were reactive to L. braziliensis antigen. Fourteen percent (8/58) of the dogs from the state of Lara were positive to L. infantum and 5.2% (3/58) to L. braziliensis antigen. Three dogs were positive to both Leishmania spp. antigens. By RT-PCR, 6.5% (4/61) and 4.4% (4/91) of the dog were positive for infection in the states of Lara and Yaracuy, respectively. The RT-PCR product of one dog from the state of Yaracuy was sequenced revealing a 100% identity with L. infantum. However, all RT-PCR positive dogs were seronegative to both Leishmania spp. antigens. In conclusion, the positivity for Leishmania spp. infections observed indicates that dogs are frequently infected by L. infantum, L. braziliensis or related Leishmania spp. in Venezuela.Elsevier B.V.2020202120202020info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/169250Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1016/j.vprsr.2020.100420Verterinary Parasitology: Regional Studies and Reports, 2020https://doi.org/10.1016/j.vprsr.2020.100420cc-by-nc-nd (c) Elsevier B.V., 2020http://creativecommons.org/licenses/by-nc-nd/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1692502026-05-29T05:05:01Z
dc.title.none.fl_str_mv Serological and molecular survey of Leishmania infection in dogs from Venezuela
title Serological and molecular survey of Leishmania infection in dogs from Venezuela
spellingShingle Serological and molecular survey of Leishmania infection in dogs from Venezuela
Kalú Rivas, Aruani
Leishmaniosi
Gossos
Veneçuela
Malalties infeccioses en els animals
Leishmaniasis
Dogs
Venezuela
Communicable diseases in animals
title_short Serological and molecular survey of Leishmania infection in dogs from Venezuela
title_full Serological and molecular survey of Leishmania infection in dogs from Venezuela
title_fullStr Serological and molecular survey of Leishmania infection in dogs from Venezuela
title_full_unstemmed Serological and molecular survey of Leishmania infection in dogs from Venezuela
title_sort Serological and molecular survey of Leishmania infection in dogs from Venezuela
dc.creator.none.fl_str_mv Kalú Rivas, Aruani
Alcover Amengual, Maria Magdalena
Martínez-Orellana, Pamela
Montserrat-Sangrà, Sara
Nachum-Biala, Yaarit
Fisa Saladrigas, Roser
Riera Lizandra, Ma. Cristina
Baneth, Gad
Solano-Gallego, Laia
author Kalú Rivas, Aruani
author_facet Kalú Rivas, Aruani
Alcover Amengual, Maria Magdalena
Martínez-Orellana, Pamela
Montserrat-Sangrà, Sara
Nachum-Biala, Yaarit
Fisa Saladrigas, Roser
Riera Lizandra, Ma. Cristina
Baneth, Gad
Solano-Gallego, Laia
author_role author
author2 Alcover Amengual, Maria Magdalena
Martínez-Orellana, Pamela
Montserrat-Sangrà, Sara
Nachum-Biala, Yaarit
Fisa Saladrigas, Roser
Riera Lizandra, Ma. Cristina
Baneth, Gad
Solano-Gallego, Laia
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Leishmaniosi
Gossos
Veneçuela
Malalties infeccioses en els animals
Leishmaniasis
Dogs
Venezuela
Communicable diseases in animals
topic Leishmaniosi
Gossos
Veneçuela
Malalties infeccioses en els animals
Leishmaniasis
Dogs
Venezuela
Communicable diseases in animals
description Venezuela is a country where human and canine leishmaniosis due to Leishmania infantum, Leishmania braziliensis and other Leishmania spp. is endemic. However, only limited data is available on canine Leishmania infection in Venezuela. The aim of this cross-sectional study was to evaluate the prevalence of Leishmania infection in dogs (n = 152) from the states of Lara (n = 91) and Yaracuy (n = 61) in Venezuela by means of serological and molecular methods. Physical examination was performed and blood samples were collected from all dogs. Serology for antibodies reactive with L. infantum and L. braziliensis antigens was assessed by the enzyme-linked immunosorbent assay (ELISA) and detection of Leishmania DNA from blood samples was evaluated by kinetoplast Leishmania real-time polymerase chain reaction (RT-PCR). In addition, Leishmania internal transcribed spacer (ITS-1) RT-PCR was performed on the samples positive by kinetoplast RT-PCR. The prevalence of Leishmania infection based on serological and/or molecular techniques was 11.8%. The seroprevalence for L. infantum and L. braziliensis antigens were 2.1% (3/144) and 8.3% (12/144), respectively. All dogs from the state of Yaracuy were serologically negative to L. infantum while 4.6% (4/86) of the dogs were reactive to L. braziliensis antigen. Fourteen percent (8/58) of the dogs from the state of Lara were positive to L. infantum and 5.2% (3/58) to L. braziliensis antigen. Three dogs were positive to both Leishmania spp. antigens. By RT-PCR, 6.5% (4/61) and 4.4% (4/91) of the dog were positive for infection in the states of Lara and Yaracuy, respectively. The RT-PCR product of one dog from the state of Yaracuy was sequenced revealing a 100% identity with L. infantum. However, all RT-PCR positive dogs were seronegative to both Leishmania spp. antigens. In conclusion, the positivity for Leishmania spp. infections observed indicates that dogs are frequently infected by L. infantum, L. braziliensis or related Leishmania spp. in Venezuela.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/169250
url https://hdl.handle.net/2445/169250
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/j.vprsr.2020.100420
Verterinary Parasitology: Regional Studies and Reports, 2020
https://doi.org/10.1016/j.vprsr.2020.100420
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Elsevier B.V., 2020
http://creativecommons.org/licenses/by-nc-nd/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Elsevier B.V., 2020
http://creativecommons.org/licenses/by-nc-nd/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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