Large-area biomolecule nanopatterns on diblock copolymer surfaces for cell adhesion studies

Cell membrane receptors bind to extracellular ligands, triggering intracellular signal transduction pathways that result in specific cell function. Some receptors require to be associated forming clusters for effective signaling. Increasing evidences suggest that receptor clustering is subjected to...

Descripción completa

Detalles Bibliográficos
Autores: Hortigüela, Verónica, Larrañaga, Enara, Lagunas, Anna, Acosta, Gerardo A., Albericio Palomera, Fernando, Andilla, Jordi, Loza Álvarez, Pablo, Martínez Fraiz, Elena
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/132326
Acceso en línea:https://hdl.handle.net/2445/132326
Access Level:acceso abierto
Palabra clave:Receptors cel·lulars
Copolímers
Biomolècules
Cell receptors
Copolymers
Biomolecules
id ES_5aed3d09cf5a28dbfdb0bdbb9a23e235
oai_identifier_str oai:diposit.ub.edu:2445/132326
network_acronym_str ES
network_name_str España
repository_id_str
spelling Large-area biomolecule nanopatterns on diblock copolymer surfaces for cell adhesion studiesHortigüela, VerónicaLarrañaga, EnaraLagunas, AnnaAcosta, Gerardo A.Albericio Palomera, FernandoAndilla, JordiLoza Álvarez, PabloMartínez Fraiz, ElenaReceptors cel·lularsCopolímersBiomolèculesCell receptorsCopolymersBiomoleculesCell membrane receptors bind to extracellular ligands, triggering intracellular signal transduction pathways that result in specific cell function. Some receptors require to be associated forming clusters for effective signaling. Increasing evidences suggest that receptor clustering is subjected to spatially controlled ligand distribution at the nanoscale. Herein we present a method to produce in an easy, straightforward process, nanopatterns of biomolecular ligands to study ligand–receptor processes involving multivalent interactions. We based our platform in self-assembled diblock copolymers composed of poly(styrene) (PS) and poly(methyl methacrylate) (PMMA) that form PMMA nanodomains in a closed-packed hexagonal arrangement. Upon PMMA selective functionalization, biomolecular nanopatterns over large areas are produced. Nanopattern size and spacing can be controlled by the composition of the block-copolymer selected. Nanopatterns of cell adhesive peptides of different size and spacing were produced, and their impact in integrin receptor clustering and the formation of cell focal adhesions was studied. Cells on ligand nanopatterns showed an increased number of focal contacts, which were, in turn, more matured than those found in cells cultured on randomly presenting ligands. These findings suggest that our methodology is a suitable, versatile tool to study and control receptor clustering signaling and downstream cell behavior through a surface-based ligand patterning technique.MDPI2019info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2445/132326Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésNanomaterials, 2019, vol. 9, p. 579http://dx.doi.org/10.3390/nano9040579info:eu-repo/grantAgreement/EC/H2020/647863cc by (c) Hortigüela et al., 2019http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1323262026-05-27T06:46:51Z
dc.title.none.fl_str_mv Large-area biomolecule nanopatterns on diblock copolymer surfaces for cell adhesion studies
title Large-area biomolecule nanopatterns on diblock copolymer surfaces for cell adhesion studies
spellingShingle Large-area biomolecule nanopatterns on diblock copolymer surfaces for cell adhesion studies
Hortigüela, Verónica
Receptors cel·lulars
Copolímers
Biomolècules
Cell receptors
Copolymers
Biomolecules
title_short Large-area biomolecule nanopatterns on diblock copolymer surfaces for cell adhesion studies
title_full Large-area biomolecule nanopatterns on diblock copolymer surfaces for cell adhesion studies
title_fullStr Large-area biomolecule nanopatterns on diblock copolymer surfaces for cell adhesion studies
title_full_unstemmed Large-area biomolecule nanopatterns on diblock copolymer surfaces for cell adhesion studies
title_sort Large-area biomolecule nanopatterns on diblock copolymer surfaces for cell adhesion studies
dc.creator.none.fl_str_mv Hortigüela, Verónica
Larrañaga, Enara
Lagunas, Anna
Acosta, Gerardo A.
Albericio Palomera, Fernando
Andilla, Jordi
Loza Álvarez, Pablo
Martínez Fraiz, Elena
author Hortigüela, Verónica
author_facet Hortigüela, Verónica
Larrañaga, Enara
Lagunas, Anna
Acosta, Gerardo A.
Albericio Palomera, Fernando
Andilla, Jordi
Loza Álvarez, Pablo
Martínez Fraiz, Elena
author_role author
author2 Larrañaga, Enara
Lagunas, Anna
Acosta, Gerardo A.
Albericio Palomera, Fernando
Andilla, Jordi
Loza Álvarez, Pablo
Martínez Fraiz, Elena
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Receptors cel·lulars
Copolímers
Biomolècules
Cell receptors
Copolymers
Biomolecules
topic Receptors cel·lulars
Copolímers
Biomolècules
Cell receptors
Copolymers
Biomolecules
description Cell membrane receptors bind to extracellular ligands, triggering intracellular signal transduction pathways that result in specific cell function. Some receptors require to be associated forming clusters for effective signaling. Increasing evidences suggest that receptor clustering is subjected to spatially controlled ligand distribution at the nanoscale. Herein we present a method to produce in an easy, straightforward process, nanopatterns of biomolecular ligands to study ligand–receptor processes involving multivalent interactions. We based our platform in self-assembled diblock copolymers composed of poly(styrene) (PS) and poly(methyl methacrylate) (PMMA) that form PMMA nanodomains in a closed-packed hexagonal arrangement. Upon PMMA selective functionalization, biomolecular nanopatterns over large areas are produced. Nanopattern size and spacing can be controlled by the composition of the block-copolymer selected. Nanopatterns of cell adhesive peptides of different size and spacing were produced, and their impact in integrin receptor clustering and the formation of cell focal adhesions was studied. Cells on ligand nanopatterns showed an increased number of focal contacts, which were, in turn, more matured than those found in cells cultured on randomly presenting ligands. These findings suggest that our methodology is a suitable, versatile tool to study and control receptor clustering signaling and downstream cell behavior through a surface-based ligand patterning technique.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/132326
url https://hdl.handle.net/2445/132326
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Nanomaterials, 2019, vol. 9, p. 579
http://dx.doi.org/10.3390/nano9040579
info:eu-repo/grantAgreement/EC/H2020/647863
dc.rights.none.fl_str_mv cc by (c) Hortigüela et al., 2019
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Hortigüela et al., 2019
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869408756825063424
score 15.300724

Similares en LA Referencia