Study of reactive oxygen species (ROS) and nitric oxide (NO) as molecular mediators of the sepsis-induced diaphragmatic contractile dysfunction : protective effect of heme oxygenases
Protein nitration is considered as a marker of reactive nitrogen species formation. Heme oxygenases (HOs) are important for the defence against oxidative stress. We evaluated the involvement of the neuronal (nNOS), the endothelial (eNOS), and the inducible (iNOS) in nitrotyrosine formation and local...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2002 |
| País: | España |
| Institución: | CBUC, CESCA |
| Repositorio: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/7066 |
| Acceso en línea: | http://www.tdx.cat/TDX-0718103-114650 http://hdl.handle.net/10803/7066 |
| Access Level: | acceso abierto |
| Palabra clave: | neuronal (nNOS) endothelial (eNOS) inducible (iNOS) 3-Nitrotyrosine Heme oxygenases (HOs). Sepsi Músculs respiratoris Espècies oxigenades reactives (ROS) Estrès oxidatiu Òxid nítric (NO) sintetases del NO (NOS) endotelial (eNOS) induïble (iNOS) 3-nitrotirosina Hemo-oxigenases (HOs) NO synthases (NOS) Nitric oxide (NO) Oxidative stress Reactive oxygen species (ROS) Respiratory muscles Sepsis 576 612 |
| Sumario: | Protein nitration is considered as a marker of reactive nitrogen species formation. Heme oxygenases (HOs) are important for the defence against oxidative stress. We evaluated the involvement of the neuronal (nNOS), the endothelial (eNOS), and the inducible (iNOS) in nitrotyrosine formation and localitzation, and both the expression and funcional significance (HO inhibition and contractility studies) of HOs in sepsis-induced muscle contractile dysfunction. Sepsis was elicited by injecting rats and transgenic mice deficient in either nNOS, eNOS, or iNOS isoforms with E.Coli lipolysaccharide (LPS). Nitrotyrosine formation and HO expressions were assessed by immunoblotting. Oxidative stress was assessed measuring protein oxidation, lipid peroxidation, and muscle glutathione. We conclude that protein tyrosine nitration occurs in normal muscles, and sepsis-mediated increase in nitrotyrosine formation is limited to the mitochondria and membrane muscle fractions. The iNOS isoform is mostly involved in nitrotyrosine formation. HOs protect normal and septic muscles from the deleterious effects of oxidants. |
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